Sub-cloning of Arabidopsis FLS2 and LysM Gene and Their Expression inTransgenic Tomatoes

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (11): 1635-1642.

• Vegetables • Previous Articles Next Articles

Sub-cloning of Arabidopsis FLS2 and LysM Gene and Their Expression inTransgenic Tomatoes

CHEN Wu¹;YANG Yu-ting¹;LI Ying²;HUANG San-wen²;LIDing-jun^1*

(¹College of Bio-safety Science and Technology, Hunan Agricultural University, Changsha 410128, China; ²Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081,China)

Received:2009-04-27 Revised:2009-06-05 Online:2009-11-25 Published:2009-11-25
Contact: LIDing-jun1

Abstract

Abstract: FLS2 and LysM genes, two receptor-like kinases (RLKs) genes, containing their own promoters, introns and transcription terminators were sub-cloned from Arabidopsis TAC library, and then were transformed into‘Moneymaker’by Agrobacterium-mediated approach. Transgenic plants were identified by
polymerase chain reaction ( PCR). RT-PCR was performed to analyze two genesp expression in transgenic plants. The results illustrated that both FLS2 and LysM genes can be transcribed with the recognition of promoters, the splicing of introns and exons by tomato transcription system. It can be deduced that the transcription mechanisms of Arabidopsis and tomato could be conservative to some extent. This study provides a theoretical base for understanding functional conservation of R genes between Arabidopsis and tomato.

Key words: Arabidopsis thaliana, tomato, FLS2, LysM, sub-cloning, expression

CLC Number:

S 641.2

CHEN Wu;YANG Yu-ting;LI Ying;HUANG San-wen;LIDing-jun. Sub-cloning of Arabidopsis FLS2 and LysM Gene and Their Expression inTransgenic Tomatoes[J]. ACTA HORTICULTURAE SINICA, 2009, 36(11): 1635-1642.

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Sub-cloning of Arabidopsis FLS2 and LysM Gene and Their Expression inTransgenic Tomatoes

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