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ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (1): 33-38.

• 蔬菜 • Previous Articles     Next Articles

Effects of Sucrose and Mannitol in washing medium on microspore embryogenesis in Chinese cabbage [Brassica rapa L .ssp. pekinensis (Lour.) Olsson]

LI Fei,ZhANG Shu-jiang,ZhANG Shi-fan,and SUN Ri-fei*   

  1. (Institute of Vegetables and Flowers , Chinese Academy of Agricultural Sciences , Beijing 100081 , China)
  • Received:2008-09-25 Revised:2008-12-10 Online:2009-01-25 Published:2009-01-25
  • Contact: SUN Ri-fei

Abstract:

The effects of short-term pretreament were studied on embryogenesis of isolated microspores in Chinese cabbage. The results showed that B5 washing medium addition mannitol and with higher concentration of sucrose during isolation of microspores were not influence microspores viability but can significantly increased the frequency of embryogenesis . In our experiments, all nine cultivars responded to the treatment. B5 washing medium with 17% sucrose treatment and B5-13 washing medium with 8% mannitol treatment were the optimal treatment of two group tests .

Key words: Chinese cabbage, microspore culture, embryogenesis, pretreament, sucrose, mannitol

CLC Number: