Identification of SSR Markers Linked to a Genic Multiple-Allele Male Sterile Gene

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (1): 103-108.

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Identification of SSR Markers Linked to a Genic Multiple-Allele Male Sterile Gene

Feng Hui^1*, Wei Peng¹, Li Cheng-yu¹, Choi Su Ryun², Lim Yong Pyo², and Piao Zhong-yun¹

(¹Department of Horticulture, Shenyang Agricultural University, Shenyang 110161; ²Plant Genome Research Center, Chungnam National University, Daejeon, 305-764, South Korea)

Received:2008-10-15 Revised:2008-12-10 Online:2009-01-25 Published:2009-01-25
Contact: Feng Hui

Abstract

Abstract:

A BC1 mapping population was constructed with Chinese cabbage inbred line "Chiifu" which is the model material of Multinational Brassica Genome Project as the male parent and genic multiple allele male sterile material 'AB01' as the female parent. Major effects of components in SSR-PCR system on amplification result were probed. 250 SSR primer pairs were employed to identify SSR markers linked to the genic multiple allele male sterile gene "Ms". Within the eight SSR primer pairs showing polymorphism between two gene bulks, two SSR markers named cnu_m273 and cnu_m295 were found linked to the Ms gene, at the genetic distances of 4.95 cM and 7.92 cM respectively.

Key words: Chinese cabbage, male sterility, multiple allele, SSR

CLC Number:

S 634.1

Feng Hui;Wei Peng;Li Cheng-yu;Choi Su Ryun;Lim Yong Pyo;and Piao Zhong-yun. Identification of SSR Markers Linked to a Genic Multiple-Allele Male Sterile Gene [J]. ACTA HORTICULTURAE SINICA, 2009, 36(1): 103-108.

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Identification of SSR Markers Linked to a Genic Multiple-Allele Male Sterile Gene

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