SDS-PAGE Analysis of Phase Change-related Proteins in Apple (Malus domestica Borhk.)

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (7): 1059-1064.

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SDS-PAGE Analysis of Phase Change-related Proteins in Apple (Malus domestica Borhk.)

ZENG Guang-Juan^1,2, LI Chun-Min¹, ZHANG Xin-Zhong^3*, TIAN Yi², CHEN Dong-Mei¹, ZHAO Yong-Bo¹,DONG Wen -Xuan²

(¹Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Sciences, Changli Hebei 066600 China;²College of Horticulture, Shenyang Agricultural University, Shenyang 110161,China;³Institute for Horticultural Plants, China Agricultural University, Beijing 100094,China)

Received:2008-01-21 Revised:2008-06-11 Online:2008-07-25 Published:2008-07-25
Contact: ZHANG Xin-Zhong

Abstract

Abstract: Dynamic changes of proteins in leaves from different nodes in 6 hybrid seedlings (Jonathan × Golden Delicious) were analyzed with SDS-polyacrylamide gel electrophoresis in apple. Three protein bands were found specific in different phases. The molecular weight of the 3 protein bands were approximately 55 kD, 19 kD and 17 kD. The 55 kD protein presented qualitatively between nodes No.36～75 and No.101～126. The change of 19 kD and 17 kD proteins was found quantitatively. In leaf samples taken below nodes No. 86～100, the content of 19 kD protein was higher, but a sharp decrease occurred in leaves above nodes No.100. The content of 17 kD protein kept high between nodes No. 36～50 and No. 91～105. It is therefore suggested that the 3 proteins were phase change-related in apple seedlings.

Key words: Malus domestica, phase change, protein, SDS-PAGE

CLC Number:

S 661.1

ZENG Guang-Juan;LI Chun-Min;ZHANG Xin-Zhong;TIAN Yi;CHEN Dong-Mei;ZHAO Yong-Bo;DONG Wen -Xuan . SDS-PAGE Analysis of Phase Change-related Proteins in Apple (Malus domestica Borhk.)[J]. ACTA HORTICULTURAE SINICA, 2008, 35(7): 1059-1064.

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SDS-PAGE Analysis of Phase Change-related Proteins in Apple (Malus domestica Borhk.)

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