Isolation and Identification of the Antagonistic Bacterial Strain ZL7-5 Against Root Rot Disease of Cymbidium hybridium

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (11): 1647-1652.

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Isolation and Identification of the Antagonistic Bacterial Strain ZL7-5 Against Root Rot Disease of Cymbidium hybridium

LI Lu-bin¹;LI Shu-na²;LI Jia²;WANG Qian²;ZHU Bao-cheng^2*;and PENG Zhen-hua^2*

(¹Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China; ²College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China)

Received:2008-05-12 Revised:2008-07-21 Online:2008-11-25 Published:2008-11-25
Contact: ZHU Bao-cheng and PENG Zhen-hua

Abstract

Abstract:

In order to obtain antagonistic strain which has the potential application possibility on biocontrol of root rot disease of Cymbidium hybridium, five hundreds and nineteen bacterial strains were isolated from the soil samples and there were twenty six bacterial strains showed antagonistic activity to Fusarium oxysporium.A strainwith the highest antagonistic activity, ZL7-5, was obtained during the secondary screening. 16S rDNA sequences analysis of strain ZL 7-5 revealed that it had 100% similarity to that of Bacillus amyloliquefaciens NBRC 15535. After the morphological, physiological and biochemical characteristics of this strain were further studied and compared with that of the type strain Bacillus amyloliquefaciens NBRC 15535, strain ZL7-5 was finally identified as Bacillus amyloliquefaciens.

Key words: Cymbidium hybridium, root rot, Fusarium oxysporium, antagonistic bacteria, screening, identification, Bacillus amyloliquefaciens

CLC Number:

S 682.31

LI Lu-bin;LI Shu-na;LI Jia;WANG Qian;ZHU Bao-cheng;and PENG Zhen-hua. Isolation and Identification of the Antagonistic Bacterial Strain ZL7-5 Against Root Rot Disease of Cymbidium hybridium[J]. ACTA HORTICULTURAE SINICA, 2008, 35(11): 1647-1652.

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Isolation and Identification of the Antagonistic Bacterial Strain ZL7-5 Against Root Rot Disease of Cymbidium hybridium

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