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ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (5): 1227-1234.

• 观赏植物 • Previous Articles     Next Articles

Phylogenetic Relationship Between Ornamental and Wild Species of Malus in China

ZHANG Ning; SHEN Hong-xiang; GAO Xia-hong; YAO Yun-cong*; WANG Yang; FENG Yong-qing 
  

  1. (Department of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

Abstract: In this study, 24 crabapple cultivars and 23 wild species of Malus were analyzed using AFLP (Amplified Fragment Length Polymorphism) molecular marker in order to test the genetic diversity and relationship of the materials. The results showed that a total of 589 informative AFLP fragments were generated with 10 EcoR Ι-Mse Ι primer combinations, of which 534 ones were polymorphic and the polymorphism rate was 90.66%. Rich genetic diversity of crabapple germplasm resources was detected at DNA level. UPGMA (Unweighted Pair-Group Method with Arithmetic Mean) cluster analysis based on Jaccard coefficient indicated that the materials used in the study could be divided into 8 groups, crabapple cultivars were divided into 4 groups. In the present study, 22 of 24 cultivars tested were closely related with species of Malus in China.

Key words: Malus crabapple, Phylogenetic relationship, Genetic diversity, AFLP