Abstract: The changing rules of endogenous hormones in the regeneration buds of Allium ovalifolium during the period from dormancy to sp routing treated with different temperatureswere determined by HPLC. The results indicated that: 1) To relieve dormancy of regeneration buds of A llium ovalifolium required a p roper period of low temperature treatment, and the accumulation of low temperatures showed a positive and marked effect on both the degree of dormancy relieving and the growing performance of the plants. A treatment of ( 3 ±1) ℃ for no less than 92 d could relieve dormancy comp letely but treatments of 5 - 12℃ and (20 ±1) ℃ could not relieve dormancy efficiently. 2) The contents of GA₃ and ZT increased and the contents ofABA decreased after storage of buds at (3 ±1) ℃ and 5 - 12℃ for 31 d, amongwhich the contents of GA₃ , ZT and ABA treated with (3 ±1) ℃ changed remarkably, resulting in a shorter period to relieve dormancy. The dormancy could not be relieved
at (20 ±1) ℃ treatment due to the increasing of ABA and the decreasing of GA₃ and ZT contents before its bulb drying rot. 3) The dormancy process of regeneration buds could be divided into three phases as predormancy,
real dormancy and forced dormancy. In the period of predormancy, the contents of GA₃ , ZT, ABA and IAA were normal. In the period of real dormancy, the contents of GA₃ and ZT were low, and the contents of IAA were not stable, but the contents of ABA were high. In the period of forced dormancy, the contents of GA₃ and ZT reached the highest and the contents of ABA dropped to the lowestwith a little increase of IAA. 4) Dormancy relieving might be controlled mainly by the contents of GA3 and ABA and the ratios of GA3 to ABA. A ratio of GA₃ /ABA≥16.59 ±3.90 could relieve the dormancy of the regeneration buds completely. More ever, the contents of ZT might also play a certain role in relieving dormancy.

Key words: Allium ovalifolium, Temperature, Regeneration buds, Dormancy, Endogenous hormones