RAPD Analysis for Genetic Assessment of Some Cultivars of Pyrus pyrifolia Derived from China and Japan

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (3): 621-624.

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RAPD Analysis for Genetic Assessment of Some Cultivars of Pyrus pyrifolia Derived from China and Japan

Shen Yuying^{1, 2};Teng Yuanwen^1*;Tanabe Kenji³

(¹Department of Horticulture, Zhejiang University, the S tate Agricultural Ministry Laboratory of Horticultural Plant Growth /Development & Biotechnology, Hangzhou, Zhejiang 310029, China; ²Department of Biotechnology, Hangzhou Wanxiang Polytechnic, Hangzhou, Zhejiang 310023, China; ³ Faculty of Agriculture, Tottori University, Tottori 680 - 8553, Japan)

Received:2005-10-18 Revised:2006-01-16 Online:2006-06-25 Published:2006-06-25

Abstract

Abstract: The present study, using RAPD analysis, was undertaken to characterize genetic variation in Japanese pears and Chinese sand pears, as well as their genetic relationships. The materials used consisted of 36 Japanese pears and 6 Chinese accessions from Zhejiang and Fujian provinces. A total of 27 primers generated 261 scorable polymorphic RAPD bands. Clustering analysis revealed 9 groups: 4 consisting of Chinese sand pears and 5 from Japan, of which 4 Japanese pear groups contained cultivars or types from Kouchi Prefecture, Japan. Chinese sand pears and Japanese pearswere notmingled together into groups due to sampling bias. However, Chinese sand pear cultivars showed higher affinities to some Japanese pears, especially those from Kouchi prefecture than to Chinese ones.

Key words: Pear, RAPD marker, Cluster analysis

Shen Yuying;;Teng Yuanwen;Tanabe Kenji. RAPD Analysis for Genetic Assessment of Some Cultivars of Pyrus pyrifolia Derived from China and Japan[J]. ACTA HORTICULTURAE SINICA, 2006, 33(3): 621-624.

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RAPD Analysis for Genetic Assessment of Some Cultivars of Pyrus pyrifolia Derived from China and Japan

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