Sequentia l Expression of Fertility-related Genes Detected by cDNA-AFLP inDifferent Types of Cabbage Male Sterile Lines

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (3): 544-548.

• 研究论文 • Previous Articles Next Articles

Sequentia l Expression of Fertility-related Genes Detected by cDNA-AFLP inDifferent Types of Cabbage Male Sterile Lines

Kang Jungen^{1, 2};Wang Xiaowu^1*;Zhang Guoyu¹;Zhang Yanguo¹;Lou Ping¹;Fang Zhiyuan¹

(¹ Institute of Vegetable and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; ²Institute of Vegetable, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi 030031, China)

Received:2005-07-07 Revised:2006-01-17 Online:2006-06-25 Published:2006-06-25

Abstract

Abstract: Elucidating the gene expression in anther development is one of the key steps to develop new types of male sterility. Four types of male sterile cabbage lines and their corresponding fertile lines were used to analyze the gene expression by cDNA-AFLP. In total, we got 113 differentially exp ressed TDFs, which were
classified into 12 group s. Among them, 76 TDFs (67.25% of the total) were classified into group A, B, C and D, which were sequentially interrupted according to the different sterile stages in four male sterile lines. Sequence analysis of representative TDFs from each group showed that glycosyl hydrolase family 1 protein, VQ motif-containing protein, glycine-rich protein and pectatelyase family protein possibly involved in sporogenesis cell, pollen mother cell, pretetrad and post-tetrad stage male development respectively.

Key words: Cabbage, Anther development, cDNA-AFLP, Gene expression

Kang Jungen;;Wang Xiaowu;Zhang Guoyu;Zhang Yanguo;Lou Ping;Fang Zhiyuan. Sequentia l Expression of Fertility-related Genes Detected by cDNA-AFLP inDifferent Types of Cabbage Male Sterile Lines[J]. ACTA HORTICULTURAE SINICA, 2006, 33(3): 544-548.

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Sequentia l Expression of Fertility-related Genes Detected by cDNA-AFLP inDifferent Types of Cabbage Male Sterile Lines

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