Genetic Diversity of Tea 〔Camellia sinensis ( L. ) O. Kuntze〕Cultivars Revealed by AFLP Analysis

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (2): 317-322.

• 研究论文 • Previous Articles Next Articles

Genetic Diversity of Tea 〔Camellia sinensis ( L. ) O. Kuntze〕Cultivars Revealed by AFLP Analysis

Huang Jian'an^{1, 2}; Li Jiaxian³;Huang Yihuan²;Luo Junwu^2*;Gong Zhihua²;Liu Zhonghua^{1, 2}

(¹Key Laboratory of Tea Science of Ministry of Education, Changsha 410128, China; ² Tea Science Department, Hunan Agricultural University, Changsha 410128, China; ³ Tea Research Institute of Guangdong, Yingde 513042, Guangdong, China)

Received:2005-03-21 Revised:2005-06-20 Online:2006-04-25 Published:2006-04-25

Abstract

Abstract: 40 tea cultivars were analyzed by AFLP (Amplified Fragment Length Polymorphism) -silver staining protocol in order to detect the genetic diversity and relationship. AFLP fingerprinting of 40 tea cultivars with primer combinations E-ACG/M-AAC, E-AGG/M-AAG and E-AGG/M-AGT revealed a total number of 226 unambiguous bands, of which 207 ones were polymorphic and the polymorphism frequency was 91.59%. This result showed the abundant diversities of restriction sites among tea cultivars studied. As analyzed by POPGENE 1.31, the average value of effective number of alleles, Nei's gene diversity and Shannonpsinformation index were 1.59 ±0.09, 0.35 ±0.04 and 0.53 ±0.05 respectively. As analyzed by SPSS12, the Nei's genetic distances of 40 tea cultivars ranged from 0.13 to 0.48, and the average distance was 0.32.
These tea cultivars were divided into four group s by UPGMA ( unweighted pair group method with arithmetic average) based on Nei's genetic distances. The genetic relationship of 40 tea cultivarswas analyzed using the similarity coefficient.

Key words: Tea plant, AFLP, Genetic diversity

Huang Jian'an;;Li Jiaxian;Huang Yihuan;Luo Junwu;Gong Zhihua;Liu Zhonghua;. Genetic Diversity of Tea 〔Camellia sinensis ( L. ) O. Kuntze〕Cultivars Revealed by AFLP Analysis[J]. ACTA HORTICULTURAE SINICA, 2006, 33(2): 317-322.

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Genetic Diversity of Tea 〔Camellia sinensis ( L. ) O. Kuntze〕Cultivars Revealed by AFLP Analysis

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