A Linkage Map Construction for Chinese Cabbage Based on AFLP, SSR, RAPD and Isozyme Markers Using DH Population

ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (02): 249-255.

• 研究论文 • Previous Articles Next Articles

A Linkage Map Construction for Chinese Cabbage Based on AFLP, SSR, RAPD and Isozyme Markers Using DH Population

Zhang Liyang^{1, 2};Zhang Fenglan¹;Wang Mei ¹;Liu Xiucun¹;Zhao Xiuyun¹;Xue Linbao²

(¹ National Engineering Research Center for Vegetables, Beijing 100089, China; ²Horticultural Department, Yangzhou University, Yangzhou 225009, China)

Received:2004-06-02 Revised:2004-10-16 Online:2005-04-25 Published:2005-04-25
Contact: Zhang Fenlan

Abstract

Abstract: A molecular genetic map for Chinese cabbage was constructed based on AFLP (Amplified Fragment Length Polymorphism) , RAPD (Random Amplified Polymorphism DNA) , SSR ( Simple Sequence Repeat) and isozyme markers. Marker analysis was performed on 100 randomly chosen DH lines obtained by
microspore culture from the F₁ between two homozygous parents: 91-112 and T12-19. 406 markers including 246 AFLP markers, 135 RAPD markers, 11 SSR markers, 12 isozyme markers, 1 SCAR and 1 morphological marker were integrated into 10 group s using JoinMap 310. It covered 826.3 cM with a mean marker interval of 2.0 cM. Number ofmarkers included in linkage group s varied from 7 to 111, mean marker interval distance from 1.0 cM to 3.8 cM and the length of linkage group s from 26.4 cM to 156.1 cM individually. A total of 45.0% distorted markers distributed in the map. The molecular genetic map constructed in this study would be useful in gene localization, comparative genomes research and QTL mapping of important agronomic traits for Chinese cabbage.

Key words: Chinese cabbage, Molecular marker, Genetic linkage map, Double haploid (DH)

CLC Number:

S634.3

Zhang Liyang;;Zhang Fenglan;Wang Mei;Liu Xiucun;Zhao Xiuyun;Xue Linbao. A Linkage Map Construction for Chinese Cabbage Based on AFLP, SSR, RAPD and Isozyme Markers Using DH Population[J]. ACTA HORTICULTURAE SINICA, 2005, 32(02): 249-255.

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A Linkage Map Construction for Chinese Cabbage Based on AFLP, SSR, RAPD and Isozyme Markers Using DH Population

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