Research on the PCR and Nested-PCR Detection of Citrus HuanglongbingPathogen

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (6): 803-806.

• 研究报告 • Previous Articles Next Articles

Research on the PCR and Nested-PCR Detection of Citrus HuanglongbingPathogen

Ding Fang^1,2;Yi Ganjun^2*;Wang Guoping^1*

(¹ Plant Science and Technology Academy , Huazhong Agriculture University , Wuhan 430070 , China ; ² Insititute of Fruit Tree Reseach , Guangdong Academy of Agricultural Science , Guangzhou 510640 , China)

Received:2004-03-03 Revised:2004-06-01 Online:2004-12-25 Published:2004-12-25

Abstract

Abstract:

The experiment was conducted with five species of citrus Huanglongbing material . On the base of the bioassay , we used PCR and Nested2PCR were used to detect precisely. The special DNA fragment was cloned and sequenced. The results showed that : both of these two PCR methods can detect the material with no obvious symptom. However the Nested-PCR was better : It could detect the Catharanthus roseas and Citrus reticulata Blanco just within one or two months after the inoculation ; but when PCR was used , the pathogen could be detected over two months after inoculation. The lowest concentration of DNA that can be detected by Nested2PCR was lower than fg , yet PCR is only pg. The sensitivity of Nested2PCR is nearly ten thousand times higher than that of PCR.

Key words: Citrus, Huanglongbing, PCR, Nested2PCR, Detection

CLC Number:

S 666

Ding Fang;Yi Ganjun;Wang Guoping. Research on the PCR and Nested-PCR Detection of Citrus HuanglongbingPathogen[J]. ACTA HORTICULTURAE SINICA, 2004, 31(6): 803-806.

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Research on the PCR and Nested-PCR Detection of Citrus HuanglongbingPathogen

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