Abstract: The ideal regeneration system of strawberry ( Fragaria ×ananassa Duch. ) ‘Toyonoka’by leaf explants was established. In this study , the effects of plant growth regulators , dark periods and AgNO₃ concentrations on shoot regeneration from leaves of strawberry were investigated. The results showed that the highest regeneration rate of 72.33 %, with 5.59 shoots per leaf disc , was obtained on the medium MS supplemented with
2.0 mg·L ^-1 TDZ and 0.8 mg·L ^-1 IBA. TDZ was more effective than BA in inducing shoot regeneration from leavesof strawberry ‘Toyonoka ’. Two weeks of dark treatment could increase the shoot regeneration rate to
90.09 %. Addition of AgNO3 to the medium could not stimulate shoot regeneration , but it changed the cell differentiational direction in somewhat from the formation of adventitious shoots to that of somatic embryos.

Key words: Strawberry, Adventitious shoots, Plant regeneration, Somatic embryogenesis