Studies on the Somatic Embryogenesis of Torreya grandis

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (5): 654-656.

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Studies on the Somatic Embryogenesis of Torreya grandis

Jiang Xinbing;Chen Ligeng^*; He Xinhua

( Department of Horticulture , Zhejiang University , the State Agriculture Minist ry Laboratory of Horticultural Plant Growth , Development and Biotechnology , Hangzhou 310029 , China)

Received:2004-01-06 Revised:2004-03-23 Online:2004-10-25 Published:2004-10-25
Contact: Chen Ligeng

Abstract

Abstract: Calli were induced from embryo of Torreya grandis , and the highest induction frequency (69. 8%) was obtained on SH basal medium supplemented with 6-A 0. mg/ L + 2,4-D 0.5 mg/ L. After subcultured for several times , somatic embryogenesis at high f requency (70.3 %) was obtained on 1/ 2 SH
medium supplemented with 6-BA 0.1 mg/ L + NAA 0.1 mg/ L. Somatic embryos can keep on growing and proliferating on appropriate medium. It is the first reported that somatic embryos were successfully cultured f rom T. grandis in the world.

Key words: Torreya grandis, Embryo, Callus, Somatic embryogenesis

CLC Number:

S 664.5

Jiang Xinbing;Chen Ligeng;He Xinhua. Studies on the Somatic Embryogenesis of Torreya grandis[J]. ACTA HORTICULTURAE SINICA, 2004, 31(5): 654-656.

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Studies on the Somatic Embryogenesis of Torreya grandis

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