Studies on Red Flesh Sport from‘Red Sun’Kiwifruit Using RAPD Marker

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (5): 511-513.

• 研究论文 • Previous Articles Next Articles

Studies on Red Flesh Sport from‘Red Sun’Kiwifruit Using RAPD Marker

Ning Yunye¹;Xiong Qing’e²;Zeng Weiguang¹;Zeng Guangrong³

(¹ Forestry and Horticultural College , Sichuan Agricultural University , Ya’an 625014 , China;²Agricultural College , Sichuan Agricultural University, Ya’an 625014 ,China ;³Guangyuan Agricultural Bureau , Guangyuan 628000 , China)

Received:2002-12-02 Revised:2003-04-30 Online:2003-10-25 Published:2003-10-25

Abstract

Abstract: Genomic DNAs from‘Red Sun’kiwifruit and its sport red-fleshed kiwifruit were amplified by RAPD with 300 102bp random primers to study the genetic substance change of the sport . Among the primers most amplification products were monomorphic , only ten of which could generate polymorphic segments. 20 out of the 148 amplified fragments were distinctive including the loss of a normal segment , appearance of a new segment and intensity changes. The Nei’s association coefficient and genetic distance between them were 0.7619 and 0.1429 respectively. The differences of amplified DNA segments showed that the red-fleshed sport was related to changes of some genetic substances. The polymorphic segments could be used to forecast the flesh color of grafted seedlings from the sport scion

Key words: Kiwifruit, Red flesh, Sport, RAPD, Forecast

CLC Number:

S 663.2

Ning Yunye;Xiong Qing’e;Zeng Weiguang;Zeng Guangrong. Studies on Red Flesh Sport from‘Red Sun’Kiwifruit Using RAPD Marker[J]. ACTA HORTICULTURAE SINICA, 2003, 30(5): 511-513.

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Studies on Red Flesh Sport from‘Red Sun’Kiwifruit Using RAPD Marker

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