Abstract: Regenerable calli and plantlets were obtained from leaf and petiole segments of Gerbera in vitro plantlets and those regenerated plantlets were morphologically normal . The rate of callus formation and rate of shoot
formation on optimum medium were as high as 96 % and 90 % separately. Four weeks’in vitro multiple times for those plantlets was 10. 85 while root formation rate was 99 % during root inducing. The earliest plantlets could be
obtained in 4 weeks. Optimal media for micropropagation after optimization experiments were MS + 62BA 3. 0 mg·L^-1 + NAA 0. 1 mg·L^-1 for induction ; MS + 62BA 3. 0 mg·L + NAA 0. 2 mg·L^-1 for multiplication of plantlets ;1/ 2 MS + IAA 1. 0 mg·L^-1 for root formation. In vitro plantlets used for explants was at the stage with 3 - 4 leaves and suitable size of cut leaf segments was about 4 mm ×4 mm.

Key words: Gerbera, Leaf, Tissue culture, Micropropagation