The Transformation of gfp in Cleopatra ( Citrus reticulata ) Mediated byAgrobacterium tumefaciens

ACTA HORTICULTURAE SINICA ›› 2002, Vol. 29 ›› Issue (2): 109-112.

• 研究论文 • Previous Articles Next Articles

The Transformation of gfp in Cleopatra ( Citrus reticulata ) Mediated byAgrobacterium tumefaciens

Shi Wei , Li Dongdong , Deng Xiuxin , and Yi Hualin

( National Key Laboratory in Genetic Improvement of Crops , Huazhong Agriculture University , Wuhan 430070 , China)

Received:1900-01-01 Revised:1900-01-01 Online:2002-04-25 Published:2002-04-25

Abstract

Abstract: The callus of Cleopatra ( Citrus reticulata) , a citrus rootstock cultivar , was transformed by the strain of Agrobacterium tumef aciens EHA105 carrying gfp gene. The callus of Cleopatra ( Citrus reticulata) was co cultivation with A. tumef aciens and selected with hygromycin , as led to the regeneration of resistant embryogenic callus and later to plantlets. The plants were verified GFP2transgenic plants by GUS analysis , GFP2fluorescent detection , PCR amplification and Southern hybridization.

Key words: Citrus, Callus, Green fluorescent protein gene ( gfp), Genetic transformation, Agrobacteriummediated

CLC Number:

S 666
Q 78

Shi Wei;Li Dongdong;Deng Xiuxin;and Yi Hualin. The Transformation of gfp in Cleopatra ( Citrus reticulata ) Mediated byAgrobacterium tumefaciens[J]. ACTA HORTICULTURAE SINICA, 2002, 29(2): 109-112.

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The Transformation of gfp in Cleopatra ( Citrus reticulata ) Mediated byAgrobacterium tumefaciens

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