Abstract: An efficiency and stable regeneration system in vitro and genetic transformation system for the commercial strawberry varieties were developed , and transgenic plants were obtained. Bud regeneration percentage of leaf pieces of strawberry varieties ‘Tudla’and ‘Senga Sengana’reached 100 %. Leaf pieces from in vitrogrown plants‘Tudla’were cocultivated for 3 days with Agrobacterium tumef aciens EHA105. After the cocultivated leaf pieces were incubated on regeneration medium
containing 40 mg/ L kanamycin for 4 weeks , the transformed buds appeared on the explants. The regeneration percentage of transformation buds of strawberry variety‘Tudla’reached 6. 8 %. The result of histochemical staining of GUS gene showed , of the 10 independent plants transformed with GUS gene , 5 expressed GUS activity strongly. The plants transformed with bar gene were able to differentiation on the medium containing glufosinate 10 mg/ L , and expressed strongly resistance to glufosinate
on field. The transgenic plants flowered and fruited normally.

Key words: Strawberry, Regeneration, Transformation, Resistance to herbicide