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Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (6): 1753-1764.doi: 10.16420/j.issn.0513-353x.2025-0529

• Plant Protection • Previous Articles     Next Articles

Pathogen Identification,Fungicide Efficacy Assessment,and Germplasm Resistance Evaluation of Anthracnose in‘Docteur Jules Guyot’Pear

LIU Sitong1,2, YAN Weiwei2, SUN Xiaonan2, ZHANG Xinnan2, TIAN Luming2, LI Yunpeng1,*(), JIA Xiaohui2,*()   

  1. 1 College of Modern Agriculture and Ecological EnvironmentHeilongjiang University, Harbin 150080, China
    2 Research Institute of PomologyChinese Academy of Agricultural Sciences,Xingcheng, Liaoning 125100, China
  • Received:2025-12-23 Revised:2026-02-12 Online:2026-06-24 Published:2026-06-24
  • Contact: LI Yunpeng, JIA Xiaohui

Abstract:

This study aimed to clarify the pathogen responsible for anthracnose in‘Docteur Jules Guyot’pear,screen effective fungicides,and identify resistant pear germplasm to provide a new basis for scientific disease control and breeding of anthracnose-resistant pear cultivars. Pathogen identification was performed through morphological observation,ITS sequencing,and verification using Koch’s postulates. In vitro toxicity determination of 24 fungicides was conducted using the mycelial growth rate method and spore germination inhibition rate method. Additionally,The lesion diameter of 76 pear germplasm accessions was determined via the mycelial disc pin-prick inoculation method. Based on the average lesion diameter method combined with cluster analysis,these germplasm accessions were categorized into five resistance grades(highly resistant,resistant,moderately resistant,susceptible,and highly susceptible)to Colletotrichum gloeosporioides. With the Euclidean distance set at 8 as the optimal cut-off point for cluster analysis,the resistance of the 76 pear germplasm accessions to C. gloeosporioides was scientifically classified. The results showed that the causal agent of‘Docteur Jules Guyot’pear anthracnose was C. gloeosporioides. Among the tested fungicides,prochloraz(an imidazole fungicide)exhibited the strongest inhibitory effect,with EC50 values of 0.047 mg · L-1 for mycelial growth and 0.046 mg · L-1 for spore germination. This was followed by carbendazim(a benzimidazoles fungicide),with EC50 values of 0.075 mg · L-1 and 0.040 mg · L-1,respectively. Comprehensive evaluation using both methods identified 13 highly resistant germplasms,with‘Wansanji’being the most resistant,followed by‘Guanhongxiao’,and 7 highly susceptible cultivars,with‘Huangguan’being the most susceptible,followed by‘Wowo’pear.

Key words: pear, anthracnose, Colletotrichum gloeosporioides, virulence assay, resistance identification