Identification，Pathogenicity Analysis，and Biological Characterization of Lasiodiplodia theobromae Causing Grapevine Canker in Ningxia Wine Grape Region

doi:10.16420/j.issn.0513-353x.2025-0382

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (8): 2454-2468.doi: 10.16420/j.issn.0513-353x.2025-0382

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Identification，Pathogenicity Analysis，and Biological Characterization of Lasiodiplodia theobromae Causing Grapevine Canker in Ningxia Wine Grape Region

PU Zhanyue¹, ZHAO Nan¹, WANG Yidong¹, XIN Ying¹^,², GU Peiwen¹^,^*()

¹ School of Agriculture， Ningxia University， Yinchuan 750021， China
² Yinchuan Forestry（Landscape）Pest Quarantine and Inspection Station， Yinchuan 750021， China

Received:2026-03-14 Revised:2026-06-02 Online:2026-08-25 Published:2026-08-25
Contact: GU Peiwen

Abstract

Abstract:

To clarify the pathogen species，pathogenicity and biological characteristics of grapevine canker in Ningxia，samples of diseased Cabernet Sauvignon and Syrah wine grape plants from five counties and cities including Yinchuan，Yongning and Qingtongxia were collected. Diseased plant samples from five counties/cities，including Yinchuan，Yongning，and Qingtongxia，covering seven wine grape cultivars：‘Cabernet Sauvignon’‘Syrah’‘Italian Riesling’‘Chardonnay’‘Merlot’‘Marselan’and ‘Viognier’. Pathogen isolation and purification were conducted using tissue isolation method. Pathogenicity of the isolated and purified strains was determined by scalding method and root irrigation method. The pathogen species were identified through morphological observation and multi-gene （ITS-TEF1-TUB2）combined phylogenetic analysis. The optimal carbon and nitrogen sources，pH value， and lethal temperature of the pathogen were determined by measuring mycelial growth and spore production. The results showed that all the strains causing the canker disease of wine grapes in Ningxia were Lasiodiplodia theobromae，among which FR2 had the strongest pathogenicity. The pathogenic strains were pathogenic to grape branches，pedicels and roots. According to the study on biological characteristics，the fungus grew and sporulated well when ammonium oxalate and ammonium acetate are used as nitrogen sources，glucose and sucrose as carbon sources，and the pH value is 5-7，with a lethal temperature of 55  ℃.

Key words: grapevine, grape canker disease, Lasiodiplodia theobromae, pathogenicity, biological characteristics

PU Zhanyue, ZHAO Nan, WANG Yidong, XIN Ying, GU Peiwen. Identification，Pathogenicity Analysis，and Biological Characterization of Lasiodiplodia theobromae Causing Grapevine Canker in Ningxia Wine Grape Region[J]. Acta Horticulturae Sinica, 2026, 53(8): 2454-2468.

Figures/Tables 11

Fig. 1 Symptoms of grapevine canker disease in the field（Cabernet Sauvignon） a：Hyperplasia and ulceration at the rootstock base；b：Necrosis and rot in both the rootstock base and root system；c：Scorch-like necrotic lesions along the leaf margins in the aboveground parts of diseased plants

Table 1 Sources and number of isolated strains of suspected pathogens of canker disease in wine grapes

分离部位Tissue of isolation	属 Genus	赤霞珠 Cabernet Sauvignon	西拉 Syrah	马瑟兰 Marselan	维欧妮 Viognier	贵人香 Italian Riesling	霞多丽 Chardonnay	美乐 Merlot	总数 Total number
根茎 Rhizome	镰刀菌属 Fusarium	6	6	6	6	4	6	4	38
	毛色二孢属 Lasiodiplodia	13	3	0	0	2	1	0	19
	茎点霉属 Phoma	1	0	0	0	0	0	0	1
	丝核菌属 Rhizoctonia	1	0	0	0	0	0	0	1
	角担菌属 Certatohasidium	1	0	0	0	0	0	0	1
	丛赤壳属 Dactylonectria	3	2	0	1	2	1	1	10
枝条 Branch	镰刀菌属 Fusarium	3	4	3	3	3	3	4	23
	毛色二孢属 Lasiodiplodia	0	0	0	0	0	0	0	0
	茎点霉属 Phoma	0	0	1	0	0	0	0	1
	丝核菌属 Rhizoctonia	0	0	0	0	0	0	0	0
	角担菌属 Certatohasidium	0	0	0	0	0	0	0	0
	丛赤壳属 Dactylonectria	1	0	0	0	0	0	0	1
果梗Stalk	镰刀菌属 Fusarium	3	3	3	2	2	2	3	18
	毛色二孢属 Lasiodiplodia	1	0	0	0	0	0	0	1
	茎点霉属 Phoma	0	0	0	0	1	0	0	1
	丝核菌属 Rhizoctonia	0	0	0	0	0	0	0	0
	角担菌属 Certatohasidium	0	0	0	0	0	0	0	0
	丛赤壳属 Dactylonectria	0	0	0	0	0	0	0	0
	总数Total number	33	18	13	12	14	13	12	115

Fig. 2 Pathogenicity validation of detached branches from seven representative suspected strains（Cabernet Sauvignon） A：Symptomatic lesion expansion on grapevine shoots；B：Lesion area measurements at 3 d and 5 d post-inoculation（n = 4）. Different lowercase letters indicate significant differences（P < 0.05）. The same below

Fig. 3 Pathogenicity assay of detached shoots from wine grape cultivars A：From left to right，each variety is the control，and the branches inoculated for 3 and 5 days，respectively；B：Area of diseased spots on branches

Fig. 4 Pathogenicity verification of wine grape clusters（Cabernet Sauvignon）

Fig. 5 Pot experiment of strain FR2（Italian Riesling） a：Symptoms of diseased plants in pot experiments；b，c：Epidermal rot of roots caused by FR2 accompanied by grayish-white mold layer；d：Necrosis and rot of lateral roots caused by FR2 under stereomicroscope；e：Cross-section of roots showing necrosis of xylem caused by FR2；f：Cross-section showing root rot and necrosis caused by FR2；g-i：Longitudinal and transverse sections showing fusiform ulcers caused by FR2；j：Pathogenic strains isolated from the root and stem of grape

Fig. 6 Morphological characteristics of Lasiodiplodia theobromae（A）and NJ phylogenetic tree based on combined ITS-EF1α-TUB sequences（B） a-c：Colony morphology；d：Pycnidia formed on PDA medium；e，f：Conidia production within pycnidia；g：Mature conidia；h：Conidial germination；i，j：Mature conidia and conidial germination；k：Paraphyses（notably elongated）；l：Pycnidium；m：Conidiophores（markedly short）

Fig. 7 Colony growth and spore production of FR2 strain under different nitrogen sources

Fig. 8 Colony growth and spore production of FR2 strain under different carbon sources

Fig. 9 Colony growth and spore production of FR2 strain at different pH values

Fig. 10 Determination of lethal temperature for FR2 strain

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Identification，Pathogenicity Analysis，and Biological Characterization of Lasiodiplodia theobromae Causing Grapevine Canker in Ningxia Wine Grape Region

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