Cloning and Expression Analysis of CmDREB Genes in Cucumis melo

doi:10.16420/j.issn.0513-353x.2025-0358

Acta Horticulturae Sinica ›› 2026, Vol. 53 ›› Issue (8): 2319-2332.doi: 10.16420/j.issn.0513-353x.2025-0358

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

Cloning and Expression Analysis of CmDREB Genes in Cucumis melo

KELAREMU · Kelimujiang, ZHANG Jian, ZHAO Zhixia, FU Huixin, ZHANG Ting^*()

Institute of Agricultural Products Processing， Xinjiang Academy of Agricultural Sciences，Xinjiang Agricultural and Sideline Products Deep Processing Engineering Technology Research Center， Urumqi 830091， China

Received:2026-03-23 Revised:2026-06-22 Online:2026-08-25 Published:2026-08-25
Contact: ZHANG Ting

Abstract

Abstract:

DREB（dehydration responsive element binding protein）is a plant-specific family of transcription factors involved in various processes such as plant growth and development，metabolism，and abiotic stress responses. However，the CmDREB genes in melon have not been systematically studied. In this study，using‘Xinmi 3’melon as the experimental material， 10 CmDREB transcription factors through genome-wide sequence analysis were identified，followed by cloning and expression analysis. The results showed that the 10 CmDREB proteins exhibited stable structures，with α-helix and random coil as the predominant secondary structures. Their amino acid lengths ranged from 179 to 428，with molecular weights ranging from 44.1 to 104.2 kD. Except for CmDREB2C，which was localized in the chloroplast，all other members were localized in the nucleus. Promoter analysis revealed that the promoter regions of all 10 CmDREB genes contained cis-acting elements responsive to low temperature，salicylic acid（SA），methyl jasmonate（MeJA），and abscisic acid（ABA）. Expression analysis further demonstrated distinct response patterns among these genes：CmDREB2C and CmDREB4C exhibited higher expression levels under low temperature，whereas CmDREB4A and CmDREB4D showed strong induction upon treatment with SA，MeJA，and ABA.

Key words: Cucumis melo, CmDREB, abiotic stress, gene cloning, expression analysis

KELAREMU · Kelimujiang, ZHANG Jian, ZHAO Zhixia, FU Huixin, ZHANG Ting. Cloning and Expression Analysis of CmDREB Genes in Cucumis melo[J]. Acta Horticulturae Sinica, 2026, 53(8): 2319-2332.

Figures/Tables 11

References 38

[1]	Cao S L, Wang Y, Li X T, Gao F, Feng J C, Zhou Y J. 2020. Characterization of the AP2/ERF transcription factor family and expression profiling of DREB subfamily under cold and osmotic stresses in Ammopiptanthus nanus. Plants, 9 (4):455. doi: 10.3390/plants9040455 URL
[2]	Chen Y F, Li Z, Sun T T, Wang D J, Wang Z T, Zhang C, Que Y X, Guo J L, Xu L P, Su Y C. 2022. Sugarcane ScDREB2B-1 confers drought stress tolerance in transgenic Nicotiana benthamiana by regulating the ABA signal,ROS level and stress-related gene expression. International Journal of Molecular Sciences, 23 (17):9557. doi: 10.3390/ijms23179557 URL
[3]	Dai Lijuan. 2024. Mechanism study on the regulation of growth and development of Populus tomentosa and response to drought stress by transcription factor PtrDREB32/33[Ph. D. Dissertation]. Harbin: Northeast Forestry University. (in Chinese)
	代丽娟. 2024. 转录因子PtrDREB32/33调控毛果杨生长发育和响应干旱胁迫的机制研究[博士论文]. 哈尔滨: 东北林业大学.
[4]	Eltayeb Mohamed Eltayeb Elhassan, Niu Xin, Su Liya, Chen Shoukun, Li Haifeng. 2019. Transcription pattern of BdDREB1G gene and its overexpression in Arabidopsis thaliana. Acta Agriculturae Boreali-occidentalis Sinica, 28 (1):89-96. (in Chinese)
	Eltayeb M E E, 牛欣, 苏丽亚, 陈守坤, 李海峰. 2019. 二穗短柄草DREB1G基因表达模式分析和在拟南芥中的异源过量表达. 西北农业学报, 28 (1):89-96.
[5]	Han Fangying, Hu Xin, Wang Nannan, Xie Yuhong, Wang Xiaoyan, Zhu Qiang. 2023. Research progress on DREBs response to abiotic stress in plants. Biotechnology Bulletin, 39 (11):86-89. (in Chinese) doi: 10.13560/j.cnki.biotech.bull.1985.2023-0124
	韩芳英, 胡昕, 王楠楠, 谢裕红, 王晓艳, 朱强. 2023. DREBs响应植物非生物逆境胁迫研究进展. 生物技术通报, 39 (11):86-89. doi: 10.13560/j.cnki.biotech.bull.1985.2023-0124
[6]	He Hongyun. 2008. The effects of low temperature stress on the growth,physiology,and biochemistry of sweet melon seedlings[M. D. Dissertation]. Shihezi: Shihezi University. (in Chinese)
	和红云. 2008. 低温胁迫对甜瓜幼苗生长及生理生化的影响[硕士论文]. 石河子: 石河子大学.
[7]	He Jiao. 2015. Evaluation of cold hardiness of different varieties of icelandic yume(Papaver nudicaule)and cloning of its cold-inducible gene PnDREB1[M. D. Dissertation]. Ya’an: Sichuan Agricultural University. (in Chinese)
	何蛟. 2015. 冰岛虞美人(Papaver nudicaule)不同品种耐寒性评价及其冷诱导基因PnDREB1的克隆[硕士论文]. 雅安: 四川农业大学.
[8]	Hu Ziang, Luo Ping, Xin Jingjing, Cui Yongyi. 2024. Identification of DREB gene family in Phalaenopsis and analysis of non biological stress expression. Journal of Agricultural Biotechnology, 32 (8):1715-1728. (in Chinese)
	胡子昂, 罗平, 辛静静, 崔永一. 2024. 蝴蝶兰DREB基因家族鉴定及非生物胁迫表达分析. 农业生物技术学报, 32 (8):1715-1728.
[9]	Je J, Chen H, Song C, Lim C O. 2014. Arabidopsis DREB2C modulates ABA biosynthesis during germination. Biochemical and Biophysical Research Communications, 452 (1):91-98. doi: 10.1016/j.bbrc.2014.08.052 URL
[10]	Krishna K R, Nagendra R, Shashi P R. 2019. Prediction and validation of DREB transcription factors for salt tolerance in Solanum lycopersicum L.:an integrated experimental and computational approach. Environmental and Experimental Botany, 165 (2019):1-18. doi: 10.1016/j.envexpbot.2019.05.015
[11]	Li H, Dong Y C, Chang J J, He J, Chen H J, Liu Q Y, Wei C H, Ma J X, Zhang Y, Yang J Q, Zhang X. 2016. High-throughput microRNA and mRNA sequencing reveals that microRNAs may be involved in melatonin-mediated cold tolerance in Citrullus lanatus L. Frontiers in Plant Science,(7):1231.
[12]	Li L B, Zhao H, Liu L, He C F, Bai R, Wang T, Yao N. 2011. Isolation and characterisation of a cold-induced DREB gene from Cymbidium insigne. The Journal of Horticultural Science and Biotechnology, 86 (1):43-49. doi: 10.1080/14620316.2011.11512723 URL
[13]	Li Shijia, Lü Zijing, Zhao Jin. 2022. Identification of R2R3-MYB subfamily genes in jujube and their expression analysis during fruit development. Agricultural Sciences in China, 55 (6):14. (in Chinese)
	李世佳, 吕紫敬, 赵锦. 2022. 枣R2R3-MYB亚家族基因鉴定及其在果实发育中的表达分析. 中国农业科学, 55 (6):14.
[14]	Liu Q N, Liu Y, Xin Z Z, Zhang D Z, Ge B M, Yang R P, Wang Z F, Yang L, Tang B P, Zhou C L. 2017. Genome-wide identification and characterization of the WRKY gene family in potato(Solanum tuberosum). Biochemical Systematics and Ecology,(71):212-218.
[15]	Liu X Q, Liu C Y, Guo Q, Zhang M, Cao B N, Xaing Z H, Zhao A C. 2015. Mulberry transcription factor MnDREB4A confers tolerance to multiple abiotic stresses in transgenic tobacco. PLos ONE, 10 (12):e0145619. doi: 10.1371/journal.pone.0145619 URL
[16]	Liu Xueqin. 2016. Study on salt and drought tolerance and MnDREB4A function of different mulberry hybrid combinations[M. D. Dissertation]. Chongqing: Southwest University. (in Chinese)
	刘雪琴. 2016. 不同桑树杂交组合耐盐耐旱性及MnDREB4A功能研究[硕士论文]. 重庆: 西南大学.
[17]	Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-∆∆CT method. Methods, 25:402-408. doi: 10.1006/meth.2001.1262 pmid: 11846609
[18]	Ma Z, Jin Y M, Wu T, Hu L, Zhang Y, Jiang W, Du X. 2022. OsDREB2B,an AP2/ERF transcription factor,negatively regulates plant height by conferring GA metabolism in rice. Front Plant Sci,(13):1007811.
[19]	Maqsood H, Munir F, Amir R, Gul A. 2022. Genome-wide identification,comprehensive characterization of transcription factors,cis-regulatory elements,protein homology,and protein interaction network of DREB gene family in Solanum lycopersicum. Frontiers in Plant Science,(13):1031679.
[20]	Mushtaq N, Munir F, Gul A, Amir R, Paracha R Z. 2021. Genome-wide analysis,identification,evolution and genomic organization of dehydration responsive element-binding(DREB)gene family in Solanum tuberosum. PeerJ,(9):e11647.
[21]	Nakano T, Suzuki K, Fujimura T, Shinshi H. 2006. Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant physiology, 140 (2):411-432. doi: 10.1104/pp.105.073783 URL
[22]	Peng Y Q, Cui L J, Wang Y, Wei L X, Geng S Y, Chen H, Chen G Y, Yang L, Bie Z L. 2024. Pumpkin CmoDREB2A enhances salt tolerance of grafted cucumber through interaction with CmoNAC1 to regulate H₂O₂ and ABA signaling and K⁺/Na⁺ homeostasis. Horticulture Research,11:uhae057.
[23]	Ram C, Verma M, Panwar B S, Arya L. 2015. Molecular cloning and in silico analysis of DREB-like gene in watermelon. Indian Journal of Horticulture, 72 (3):338-342. doi: 10.5958/0974-0112.2015.00066.3 URL
[24]	Sakuma Y, Liu Q, Dubouzet J G, Abe H, Shinozaki K, Yamaguchi-Shinozaki K. 2002. DNA-binding specificity of the ERF/AP 2 domain of Arabidopsis DREBs,transcription factors involved in dehydration-and cold-inducible gene expression. Biochemical and Biophysical Research Communications, 290 (3):998-1009. doi: 10.1006/bbrc.2001.6299 pmid: 11798174
[25]	Shen Yanhui. 2023. The role and regulatory mechanism of GhDREB1 and GhNAC029 in cotton low-temperature response[M. D. Dissertation]. Beijing: Chinese Academy of Agricultural Sciences. (in Chinese)
	申延会. 2023. GhDREB1和GhNAC029在棉花低温响应中的作用及调控机制[硕士论文]. 北京: 中国农业科学院.
[26]	Tang Xun, Guan Yalin, Chen Lingling, Xia Dexin, Song Chunhua, Liu Liyan, Chang Ying. 2020. Cloning and expression of DfDREB gene from Dryopteris fragrans. Acta Bot Boreal, 40 (7):1105-1113. (in Chinese)
	汤珣, 官亚琳, 陈玲玲, 夏德鑫, 宋春华, 刘丽艳, 常缨. 2020. 香鳞毛蕨DfDREB基因克隆与表达模式分析. 西北植物学报, 40 (7):1105-1113.
[27]	Wan Ye. 2023. Identification,expression,and functional verification of the grape DREB transcription factor family and VaDREB gene[M. D. Dissertation]. Yangling: Northwest A & F University. (in Chinese)
	万叶. 2023. 葡萄DREB转录因子家族的鉴定,表达及VaDREB基因功能验证[硕士论文]. 杨凌: 西北农林科技大学.
[28]	Wang C, Han J, Wang T, Chen C H, Liu J Y, Xu Z X, Zhang Q X, Wang L N, Ren Z H. 2022. Pan-genome-wide identification and transcriptome-wide analysis of DREB genes that respond to biotic and abiotic stresses in cucumber. Agriculture, 12 (11):1879. doi: 10.3390/agriculture12111879 URL
[29]	Wang Di. 2022. Study on salt tolerance function of European plum transcription factor ChDREB2C[M. D. Dissertation]. Harbin: Northeast Forestry University. (in Chinese)
	王迪. 2022. 欧李转录因子ChDREB2C的耐盐功能研究[硕士论文]. 哈尔滨: 东北林业大学.
[30]	Wang Tao. 2021. Synergistic regulation of Arabidopsis thaliana response to ABA by E3 ubiquitin ligase DEL1/2 and transcription factor DREB2C[M. D. Dissertation]. Chengdu: Sichuan University. (in Chinese)
	王韬. 2021. E3泛素连接酶DEL1/2和转录因子DREB2C协同调控拟南芥对ABA的响应研究[硕士论文]. 成都: 四川大学.
[31]	Xu Y, Zhang Y S, Ma F N, Zhao J X, Yang H T, Song S, Zhang S L. 2024. Identification of DREB family genes in banana and their function under drought and cold stress. Plants, 13 (15):2119. doi: 10.3390/plants13152119 URL
[32]	Yuan G P, He Y, Sun D X, Shi M K, Li W H, Zhang J Y, Zhu Y C. 2026. ClaDREB14 enhances the salt tolerance of watermelon by positively regulating the expression of ClaPOD6. Horticultural Plant Journal, 12 (2):414-430. doi: 10.1016/j.hpj.2025.02.014 URL
[33]	Zhang X W, Fu X, Liu F J, Wang Y A, Bi H G, Ai X Z. 2021. Hydrogen sulfide improves the cold stress resistance through the CsARF5-CsDREB3 module in cucumber. International Journal of Molecular Sciences, 22 (24):13229. doi: 10.3390/ijms222413229 URL
[34]	Zhang Z L, Wei L, Gao X M, Xv M M, Guo Y F. 2020. DEAR4,a member of DREB/CBF family,positively regulates leaf Senescence and response to multiple stressors in Arabidopsis thaliana. Frontiers in Plant Science, 11:1-18. doi: 10.3389/fpls.2020.00001 URL
[35]	Zhao T, Xia H, Liu J Y, Ma F W. 2014. The gene family of dehydration responsive element-binding transcription factors in grape(Vitis vinifera),genome-wide identification and analysis,expression profiles,and involvement in abiotic stress resistance. Molecular Biology Reports,(41):1577-1590.
[36]	Zhou Y X, Zhou W, Liu H, Liu P, Li Z G. 2020. Genome-wide analysis of the soybean DREB gene family:identification,genomic organization and expression profiles in response to drought stress. Plant Breeding, 139 (6):1158-1167. doi: 10.1111/pbr.v139.6 URL
[37]	Zhou Yan. 2023. Preliminary study on the mechanism of MrDREB1 regulating the response of Medicago rutaecarpa to cold stress[M. D. Dissertation]. Hohhot: Inner Mongolia University. (in Chinese)
	周艳. 2023. MrDREB1调控扁蓿豆响应冷胁迫机制的初步研究[硕士论文]. 呼和浩特: 内蒙古大学.
[38]	Zuo Zhifang, Li Yonglong, Wei Yüjia, Zhou Shenghui, Li Yan, Yang Guofeng. 2025. Identification of DREB genes from Zoysia japonica and their transcript profiles in response to abiotic stress. Acta Prataculturae sinica, 34 (5):74-88. (in Chinese) doi: 10.11686/cyxb2024257
	左志芳, 李永龙, 魏雨佳, 周生辉, 李岩, 杨国锋. 2025. 结缕草DREB基因家族的鉴定及非生物胁迫下的表达模式分析. 草业学报, 34 (5):74-88. doi: 10.11686/cyxb2024257

引物名称 Primer name	序列（5′-3′） Sequence	引物名称 Primer name	序列（5′-3′） Sequence
4268F	ATGGCAAAACCAAAGAATTCAAATCC	83P2F	ATGGTCGAGAAAATGGGTCACAGTG
4268R	TTAATCCCATAGTACGCCGTAA	83P2R	TCAAAATCCTAAATCACCAATTTCGTTCAG
9763F	ATGCCTAAATTAGGAAAACTAC	0977F	ATGTTGAAATCTTGTTCATCCAAC
9763R	CTAGACATCTTCAACTGCAC	0977R	TTAATACCATGGGATTGAAGAATCCC
4579F	ATGGCCAACGCCACCGT	4714F	ATGGAGAACGATTCTACAATTTTAAACT
4579R	TTACCACGAGAAAGATAAAGG	4714R	TTAATTTTGCCATAAGAAAGTATCGG
7769F	ATGGCAGCAGCTATAGATG	6024F	ATGACGTCTGAGAATTCTAGTGGA
7769R	TTAACACTCTGATTCGGCCA	6024R	TCATCCATGGAAGCATAAATCAAGC
83P1F	ATGATATCAGAGACGATTGAGA	1143F	ATGAGTAGTAGTAAAGAACAGA
83P1R	TCAAAATCCTAAATCACCAATTTCG	1143R	TCAATAATCAAACAGAAACCC

引物名称 Primer name	序列（5′-3′） Sequence	引物名称 Primer name	序列（5′-3′） Sequence
4268F	ATGGCAAAACCAAAGAATTCAAATCC	83P2F	ATGGTCGAGAAAATGGGTCACAGTG
4268R	TTAATCCCATAGTACGCCGTAA	83P2R	TCAAAATCCTAAATCACCAATTTCGTTCAG
9763F	ATGCCTAAATTAGGAAAACTAC	0977F	ATGTTGAAATCTTGTTCATCCAAC
9763R	CTAGACATCTTCAACTGCAC	0977R	TTAATACCATGGGATTGAAGAATCCC
4579F	ATGGCCAACGCCACCGT	4714F	ATGGAGAACGATTCTACAATTTTAAACT
4579R	TTACCACGAGAAAGATAAAGG	4714R	TTAATTTTGCCATAAGAAAGTATCGG
7769F	ATGGCAGCAGCTATAGATG	6024F	ATGACGTCTGAGAATTCTAGTGGA
7769R	TTAACACTCTGATTCGGCCA	6024R	TCATCCATGGAAGCATAAATCAAGC
83P1F	ATGATATCAGAGACGATTGAGA	1143F	ATGAGTAGTAGTAAAGAACAGA
83P1R	TCAAAATCCTAAATCACCAATTTCG	1143R	TCAATAATCAAACAGAAACCC

引物名称 Primer name	序列（5′-3′） Sequence	引物名称 Primer name	序列（5′-3′） Sequence
Q4268F	TGAGTGAGATCGTCGAGTTG	Q83P2R	GATCCTTTAGCTGGAGCTTTACGG
Q4268R	GTACGCCGTAAAAACTGCTC	Q0977F	GGAGGTCCTGAAAATGCTTC
Q9763F	CTCGTAGAGTTCCAGCCAAG	Q0977R	TCAGAGCCATAAAGACGACG
Q9763R	GAAATGTACCCAACCAGAGC	Q4714F	ATTTTAAACTCATCTCCGCCG
Q4579F	GTAGTATTGCCGAGTTTGGG	Q4714R	GAAACCCATTTTCCCCATGC
Q4579R	AGTGGGAATCACACACTCTC	Q6024F	GGAGGAGTAGAGGAAACTCG
Q7769F	GGTTTTGAGCAATCAGGTCC	Q6024R	GTTACTCGCAATTGGCTCTC
Q7769R	GTAGGTTTAGGTGGAGAGCC	Q1143F	GTGAGATTGTTGAGTTGCCG
Q83P1F	CTCTACGTGTTCCGAGTCCACTACTG	Q1143R	TCCCATTCCCAACTTACCAC
Q83P1R	CAATCCTCATGTTTCACTTGATTCTC	EF1aF	AAATACTCCAAGGCAAGGTAC
Q83P2F	GGTCACAGTGATCAATATTCTG	EF1aR	TCATGTTGTCACCCTCGAAACCAG

引物名称 Primer name	序列（5′-3′） Sequence	引物名称 Primer name	序列（5′-3′） Sequence
Q4268F	TGAGTGAGATCGTCGAGTTG	Q83P2R	GATCCTTTAGCTGGAGCTTTACGG
Q4268R	GTACGCCGTAAAAACTGCTC	Q0977F	GGAGGTCCTGAAAATGCTTC
Q9763F	CTCGTAGAGTTCCAGCCAAG	Q0977R	TCAGAGCCATAAAGACGACG
Q9763R	GAAATGTACCCAACCAGAGC	Q4714F	ATTTTAAACTCATCTCCGCCG
Q4579F	GTAGTATTGCCGAGTTTGGG	Q4714R	GAAACCCATTTTCCCCATGC
Q4579R	AGTGGGAATCACACACTCTC	Q6024F	GGAGGAGTAGAGGAAACTCG
Q7769F	GGTTTTGAGCAATCAGGTCC	Q6024R	GTTACTCGCAATTGGCTCTC
Q7769R	GTAGGTTTAGGTGGAGAGCC	Q1143F	GTGAGATTGTTGAGTTGCCG
Q83P1F	CTCTACGTGTTCCGAGTCCACTACTG	Q1143R	TCCCATTCCCAACTTACCAC
Q83P1R	CAATCCTCATGTTTCACTTGATTCTC	EF1aF	AAATACTCCAAGGCAAGGTAC
Q83P2F	GGTCACAGTGATCAATATTCTG	EF1aR	TCATGTTGTCACCCTCGAAACCAG

名称 Name	基因ID Gene ID	氨基酸数量 No. of amino acid	不稳定系数 Coefficient of instability	等电点 pI	平均亲水指数 Grand average of hydropathicity	分子量/kD Molecular weight	亚细胞定位 Prediction of subcellular localization
CmDREB4A	MELO3C011143P1	229	34.13	5.16	0.662	57.6	细胞核Nuclear
CmDREB4B	MELO3C024268P1	201	39.68	5.14	0.800	49.7	细胞核Nuclear
CmDREB4C	MELO3C004579P1	252	42.04	5.08	0.878	63.3	细胞核Nuclear
CmDREB4D	MELO3C024714P1	250	58.21	5.08	0.910	64.3	细胞核Nuclear
CmDREB6A	MELO3C007769P1	349	56.57	5.02	0.860	90.4	细胞核Nuclear
CmDREB2A	MELO3C010977P1	211	51.30	5.19	0.641	53.8	细胞核Nuclear
CmDREB2B	MELO3C026024P1	373	32.06	5.11	0.651	90.5	细胞核Nuclear
CmDREB2C	MELO3C019763P1	179	41.57	5.22	0.754	44.1	叶绿体Chloroplast
CmDREB2D	MELO3C008318P1	413	39.59	5.06	0.710	100.2	细胞核Nuclear
CmDREB2E	MELO3C008318P2	428	40.02	5.06	0.713	104.2	细胞核Nuclear

Cloning and Expression Analysis of CmDREB Genes in Cucumis melo

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 11

References 38

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YANG Hongyu, WANG Yan, LIU Taotao, YAO Wenxia, FAN Xiangjun, YU Bin, SHI Guiying. Cloning of the StTrihelix30 Gene in Potatoes and Functional Analysis Under Low-temperature Stress [J]. Acta Horticulturae Sinica, 2026, 53(8): 2306-2318.
[2]	CAI Zeyu, LI Ai, ZHU Jiawei, DENG Man, JIN Yuxuan, ZHANG Weihua, WU Ying. Cloning and Functional Analysis of Citrullus lanatus Ovate Family Protein Gene ClOFP16 [J]. Acta Horticulturae Sinica, 2026, 53(5): 1329-1342.
[3]	CAO Lei, DU Ping, YANG Riling, ZHOU Yuhao, HOU Juan, LI Xiang, WANG Panqiao, LI Lili, LUO Chen, LI Qiong, MAO Wenwen, HU Jianbin. Identification and Low Temperature Stress Responsive Expression Analysis of TIFY Gene Family in Melon [J]. Acta Horticulturae Sinica, 2026, 53(5): 1343-1356.
[4]	HUANG Jiuyan, DUAN Xiaoqian, FU Yue, CHANG Wei, DING Yong. Cloning of FAD2 Genes in Three Paphiopedilum Species and Comparative Analysis of Their Expression in Responses to Chilling Stress [J]. Acta Horticulturae Sinica, 2026, 53(5): 1371-1381.
[5]	BAI Xueying, TANG Manzhi, QU Tianhui, ZHANG Liting, XIAO Dong, LI Ying, HOU Xilin, LIU Tongkun. Identification of FRL Gene Family，Expression Analysis and Functional Study of BrcFRI1 in Non-Heading Chinese Cabbage [J]. Acta Horticulturae Sinica, 2026, 53(4): 1125-1142.
[6]	DAI Yuru, SUN Pingping, WANG Qiong. Genome-Wide Identification of GAD Gene Family in Tomato and Expression Profiling after Ralstonia solanacearum Infection [J]. Acta Horticulturae Sinica, 2026, 53(4): 1157-1174.
[7]	REN Jiaxuan, ZHANG Fan, WANG Chenbing. Identification of the Peach SAP Gene Family and Alleviative Response in Rootstocks Under Abiotic Stress [J]. Acta Horticulturae Sinica, 2026, 53(3): 635-652.
[8]	CHEN Yushan, GAO Huan, WU Junkang, WANG Limei, HUANG Chunhui, XU Xiaobiao, LIAO Guanglian. Identification and Expression Analysis of AeBAM Gene Family in Actinidia eriantha [J]. Acta Horticulturae Sinica, 2026, 53(3): 683-696.
[9]	CHEN Yiquan, FAN Ronghui, LIN Bing, CHEN Yan, WU Jianshe, ZHONG Huaiqin. Screening and Validation of Reference Genes for qRT-PCR Analysis of Genes Related to Floral Scent Biosynthesis in Camellia japonica [J]. Acta Horticulturae Sinica, 2026, 53(2): 598-612.
[10]	ZHU Xuanyi, ZHAO Yuqing, TANG Feihong, WU Haiyan, ZHANG Chun, ZHAO Kai, PENG Donghui, LAN Siren, ZHOU Yuzhen. Whole-Genome Identification of the CNGC Gene Family in Cymbidium ensifolium and Transcriptional Reponses Under Abiotic Stresses [J]. Acta Horticulturae Sinica, 2026, 53(1): 131-148.
[11]	WANG Yanqiu, BAN Jingjie, ZENG Yuhan, LAI Chunwang, HUANG Xiaoling, ZHOU Jianjin, WAN Kui, LAI Zhongxiong, LIN Yuling. Identification of the DREB Gene Family in Polygonatum cyrtonema and Analysis of Its Response to Salt Stress [J]. Acta Horticulturae Sinica, 2026, 53(1): 159-173.
[12]	WANG Baochen, LUO Chen, YAN Jinqiang, CHENG Xiaoxin, MO Renlian, LIU Wenrui, ZHANG Yuyang, JIANG Biao. Cloning，Expression，and Functional Research of BhYAB4b in Wax Gourd [J]. Acta Horticulturae Sinica, 2025, 52(9): 2353-2362.
[13]	ZHANG Ting, WANG Botao, ZHANG Lei, SONG Lihua, CAO Bing, MA Yaping. Identification of the LbWaxy Gene Family in Lycium barbarum and Analysis of Gene Expression Characteristics Under High Concentrations of CO₂ [J]. Acta Horticulturae Sinica, 2025, 52(9): 2395-2409.
[14]	ZHANG Ruiyi, FENG Wenjia, LI Benben, SONG Yiying, WEI Mingyue, CHAI Guaiqiang, and HUO Yanbo. Cloning and Functional Analysis of Cuticular Wax Synthesis Gene SlCER1-7 in Tomato [J]. Acta Horticulturae Sinica, 2025, 52(7): 1733-1744.
[15]	ZHANG Tuo, NIE Menghan, LIU Yuanyuan, DUAN Xinlian, HAN Xing, XIE Baogui, and YAN Junjie. Expression Analysis of the Catalase Genes FfCAT1 and FfCAT2 in Flammulina filiformis [J]. Acta Horticulturae Sinica, 2025, 52(7): 1769-1779.