MiR169a Regulates the Stomatal Characteristics of Potatoes to Adapt to the Dynamic Environment

doi:10.16420/j.issn.0513-353x.2024-0962

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (11): 2889-2898.doi: 10.16420/j.issn.0513-353x.2024-0962

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

MiR169a Regulates the Stomatal Characteristics of Potatoes to Adapt to the Dynamic Environment

ZHANG Hongguan¹^,³, LEI Ziqian¹, WANG Ming¹, ZHANG Xingyuan¹, MAO Jun¹, LIN Yuan¹^,², HU Xinxi¹^,², XIONG Xingyao¹^,², and QIN Yuzhi¹^,²^,^*()

¹ Hunan Provincial Key Laboratory of Vegetable Biology， College of Horticulture，Hunan Agricultural University， Changsha 410128， China
² Yuelu Mountain Laboratory， Changsha 410128， China
³ Shaoyang Academy of Agricultural Sciences， Shaoyang， Hunan 422000， China

Received:2025-08-06 Revised:2025-10-09 Online:2025-11-26 Published:2025-11-26
Contact: and QIN Yuzhi

Abstract

Abstract:

To verify the specific function of potato microRNA169a（miR169a）in stomata，potato miR169a overexpression（OE169a）and silenced（STTM169）lines were constructed，using the wild type as a control，and measured relevant parameters. The results showed that OE169a increased the stomatal aperture of potatoes under dynamic environmental conditions，while reducing their net photosynthetic rate （P_n），intercellular carbon dioxide concentration（C_i），intrinsic water use efficiency（iWUE），and antioxidant capacity. In contrast，STTM169 decreased the stomatal aperture under dynamic environmental conditions and enhanced P_n，C_i，iWUE and antioxidant capacity. In conclusion, silencing miR169 reduces the stomatal aperture of potatoes under dynamic environmental conditions and helps maintain higher light and water use efficiency under stress，thereby improving potato adaptability to dynamic environments.

Key words: miR169, stomata, dynamic environment, photosynthetic characteristics, potato

ZHANG Hongguan, LEI Ziqian, WANG Ming, ZHANG Xingyuan, MAO Jun, LIN Yuan, HU Xinxi, XIONG Xingyao, and QIN Yuzhi. MiR169a Regulates the Stomatal Characteristics of Potatoes to Adapt to the Dynamic Environment[J]. Acta Horticulturae Sinica, 2025, 52(11): 2889-2898.

Figures/Tables 10

References 26

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名称 Name	序列 Sequence
StActin-F	ATTCAAGTATGCCTGGGTGCT
StActin-R	GTGGTGGAGTCAATAATGAGGAC
St169a-F	TCTTCGTCAACATGGTGGAGCACGA
St169a -R	GCGATTAAGTTGGGTAACGCCAGGG

名称 Name	序列 Sequence
StActin-F	ATTCAAGTATGCCTGGGTGCT
StActin-R	GTGGTGGAGTCAATAATGAGGAC
St169a-F	TCTTCGTCAACATGGTGGAGCACGA
St169a -R	GCGATTAAGTTGGGTAACGCCAGGG

株系 Lines	气孔密度/（个 · mm^-2） Stomatal density	气孔长度/μm Stomatal length	气孔宽度/μm Stomatal width
WT	156.15 ± 2.52 b	26.17 ± 0.76 a	33.50 ± 2.50 a
OE169a	261.46 ± 2.02 a	24.25 ± 1.75 a	35.75 ± 2.25 a
STTM169	86.62 ± 3.06 c	25.50 ± 0.50 a	31.50 ± 5.10 a

株系 Lines	气孔密度/（个 · mm^-2） Stomatal density	气孔长度/μm Stomatal length	气孔宽度/μm Stomatal width
WT	156.15 ± 2.52 b	26.17 ± 0.76 a	33.50 ± 2.50 a
OE169a	261.46 ± 2.02 a	24.25 ± 1.75 a	35.75 ± 2.25 a
STTM169	86.62 ± 3.06 c	25.50 ± 0.50 a	31.50 ± 5.10 a

株系 Lines	净光合速率/ （μmol · m^-2· s^-1） Net photosynthetic rate	气孔导度/ （mmol · m^-2· s^-1） Stomatal conductance	胞间二氧化碳浓度/ （μmol · mol^-1） Intercellular CO₂concentration	蒸腾速率/ （mmol · m^-2· s^-1） Transpiration rate	内部水分利用率/% Intrinsic water use effficiency	SPAD值 SPAD value
WT	16.29 ± 0.23 b	0.14 ± 0 b	263.20 ± 7.06 b	4.55 ± 0.42 b	115.03 ± 4.77 b	30.23 ± 0.55 a
OE169a	18.15 ± 1.21 a	0.19 ± 0.01 a	278.77 ± 6.26 a	5.72 ± 0.38 a	93.83 ± 7.92 b	29.37 ± 0.64 a
STTM169	13.63 ± 0.52 c	0.07 ± 0 c	199.21 ± 6.61 c	2.53 ± 0.32 c	196.50 ± 26.04 a	29.77 ± 0.58 a

MiR169a Regulates the Stomatal Characteristics of Potatoes to Adapt to the Dynamic Environment

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株系 Lines	净光合速率/ （μmol · m^-2 · s^-1） Net photosynthetic rate	气孔导度/ （mmol · m^-2·s^-1） Stomatal conductance	胞间二氧化碳浓度/ （μmol · mol^-1） Intercellular CO₂ concentration	蒸腾速率/ （mmol · m^-2 · s^-1） Transpiration rate	内部水分利用率/% Intrinsic water use effficiency	SPAD值/ Units SPAD value
WT	4.35 ± 0.20 b	0.12 ± 0.01 b	161.35 ± 4.60 b	1.38 ± 0.23 b	35.87 ± 4.57 b	37.93 ± 4.86 b
OE169a	3.00 ± 0.94 c	0.16 ± 0.01 a	135.17 ± 3.66 c	1.90 ± 0.10 a	18.91 ± 6.27 b	28.82 ± 1.79 c
STTM169	6.38 ± 0.71 a	0.04 ± 0.01 c	178.06 ± 6.49 a	0.64 ± 0.12 c	175.99 ± 27.38 a	45.36 ± 6.05 a

株系 Lines	POD / （U · min^-1 · g^-1 FW）	CAT / （U · min^-1 · g^-1 FW）	SOD / （U · min^-1 · g^-1 FW）	H₂O₂/ （μmol · g^-1 FW）	MDA / （μmol · g^-1 FW）
WT	10.45 ± 0.73 b	232.92 ± 21.42 b	133.78 ± 4.58 b	82.31 ± 6.46 b	43.33 ± 1.37 b
OE169a	9.57 ± 0.22 b	132.26 ± 5.01 c	178.20 ± 2.85 a	133.28 ± 4.21 a	52.17 ± 1.76 a
STTM169	19.52 ± 0.17 a	322.87 ± 10.09 a	134.23 ± 4.21 b	67.34 ± 0.91 c	45.30 ± 1.79 b

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