Understanding the Anabolic Differences of Steroidal Glycoalkaloids in Leaf Between Two Potato Varieties Grown at the Winter and Spring Cropping Patterns

doi:10.16420/j.issn.0513-353x.2024-0667

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (7): 1883-1900.doi: 10.16420/j.issn.0513-353x.2024-0667

• Cultivation·Physiology & Biochemistry • Previous Articles Next Articles

Understanding the Anabolic Differences of Steroidal Glycoalkaloids in Leaf Between Two Potato Varieties Grown at the Winter and Spring Cropping Patterns

ZHU Xingzhe¹, SU Xianyue¹, SU Wang², ZHANG Honglin¹, PAN Zhechao³, LIU Tao¹, and XU Xiaoyu¹^,^**()

¹ College of Agronomy and Biotechnology，Yunnan Agricultural University，Kunming 650201，China
² Qinghai University，Xining 810016，China
³ Institute of Cash crop，Yunnan Academy of Agricultural Sciences，Kunming 650205，China

Received:2024-10-07 Revised:2025-04-18 Online:2025-07-23 Published:2025-07-23
Contact: and XU Xiaoyu

Abstract

Abstract:

To investigate the impacts of habitat discrepancy on the steroidal glycoalkaoids（SGA）anabolism in the leaves of different potato genotypes under the winter（W）and spring（S）cropping patterns in Yunnan Province，the Qingshu 9（Q9）and Lishu 6（L6）potato varieties were chosen as the major experimental materials for the joint comparative proteomic and metabolomic analysis. Alterations occurred on the leaf SGA metabolism have been preliminarily characterized at the key tuber growing stages，when the two potato varieties were cultivated under the winter and spring cropping patterns，respectively. Results indicated that：（1）Total contents of SGA in the potato leaves displayed an uprising trend with the tuber maturation，but had the most obvious significant differences at the tuber bulking stage，which was thereby selected for omics research based on the two comparison modes（Q9-leafW vs. Q9-leafS；Q9-leafW vs. L6-leafW）（2）It was found that the genotypic difference and cropping pattern induced habitat discrepancy had greatly disturbed the overall protein expression regulation and the resulted metabolites repartitioning in potato leaves on both primary and secondary metabolic levels.（3）There were a total of four differentially expressed proteins and accumulated metabolites，which are highly associated with the SGA biosynthesis，identified in the two comparison modes，respectively. Their diversified functions exhibited distinct regulatory effects on the composition and content variations in the potato leaf SGA across the primary and secondary metabolic phases of the SGA biosynthesis.

Key words: potato, cropping pattern, leaf, SGA, omics

ZHU Xingzhe, SU Xianyue, SU Wang, ZHANG Honglin, PAN Zhechao, LIU Tao, and XU Xiaoyu. Understanding the Anabolic Differences of Steroidal Glycoalkaloids in Leaf Between Two Potato Varieties Grown at the Winter and Spring Cropping Patterns[J]. Acta Horticulturae Sinica, 2025, 52(7): 1883-1900.

Figures/Tables 8

References 52

[16]	Levina A V, Hoekenga O A, Gordin M L, Broeckling C D, de Jong W S. 2023. Applying network and genetic analysis to the potato metabolome. Frontiers in Plant Science,14:1108351.
[17]	Li Chongguang, Liu Yiqiang. 2023. High-yield cultivation of winter and spring potato Lishu No. 6. Yunnan Agriculture, (3):64-66. (in Chinese)
	李崇光, 刘一强. 2023. 冬春马铃薯丽薯6号高产栽培. 云南农业,(3):64-66.
[18]	Li D W, Lu X J, Zhu Y H, Pan J, Zhou S Q, Zhang X Y, Zhu G T, Shang Y, Huang S W, Zhang C Q. 2022. The multi-omics basis of potato heterosis. Journal of Integrative Plant Biology, 64 (3):671-687. doi: 10.1111/jipb.13211
[19]	Li Hongyan, Li Jieya, Ye Guangji, Su Wang, Zhou Yun, Wang Jian. 2022. Analysis of SGAs content and gene expression in potato tissue. Southwest China Journal of Agricultural Science, 35 (2):271-277. (in Chinese)
	李红艳, 李洁雅, 叶广继, 苏旺, 周云, 王舰. 2022. 马铃薯组织中SGAs含量差异及其基因表达分析. 西南农业学报, 35 (2):271-277.
[20]	Li Qing, Zheng Zhouyi, Liu Yuting, Zhao Yarui, Wang Chenhui, Zou Wanjun, Li Zhou, Tang Wei, Li Canhui. 2023. Analysis of pathogens and pathogenicity characteristics of potato early blight in Yunnan Province. Microbiology China, 50 (2):471-485. (in Chinese)
	李清, 郑周宜, 刘雨婷, 赵娅锐, 王晨晖, 邹万君, 李周, 唐唯, 李灿辉. 2023. 云南省马铃薯早疫病病原及毒性特点分析. 微生物学通报, 50 (2):471-485.
[21]	Li Yuzhu, Guo Huachun, Wang Qiong. 2020. Accumulation of steroidal glycoalkaloids in organs of different potato varieties. Science and Technology of Food Industry, 41 (22):1-7,13. (in Chinese)
	李玉珠, 郭华春, 王琼. 2020. 马铃薯不同品种各器官糖苷生物碱累积规律研究. 食品工业科技, 41 (22):1-7,13.
[22]	Matoba Y, Kihara S, Bando N, Yoshitsu H, Sakaguchi M, Kayama K, Yanagisawa S, Ogura T, Sugiyama M. 2018. Catalytic mechanism of the tyrosinase reaction toward the Tyr 98 residue in the caddie protein. PLOS Biology, 16 (12):e3000077.
[23]	Monribot-Villanueva J L, Altúzar-Molina A, Aluja M, Zamora-Briseño J A, Elizalde-Contreras J M, Bautista-Valle M V, Arellano de losSantos J, Sánchez-Martínez D E, Rivera-Reséndiz F J, Vázquez-Rosas-Landa M, Camacho-Vazquez C, Guerrero-Analco J A, Ruíz-May E. 2022. Integrating proteomics and metabolomics approaches to elucidate the ripening process in white Psidium guajava. Food Chemistry,367:130656.
[24]	Nicolás-Espinosa J, García-Ibáñez P, Lopez-Zaplana A, Yepes-Molina L, Albaladejo-Marico L, Carvajal M. 2023. Confronting secondary metabolites with water uptake and transport in plants under abiotic stress. International Journal of Molecular Sciences, 24 (3):2826.
[25]	Nkwe D O, Lotshwao B, Rantong G, Matshwele J T P, Kwape T E, Masisi K, Gaobotse G, Hefferon K, Makhzoum A. 2021. Anticancer mechanisms of bioactive compounds from Solanaceae:an update. Cancers, 13 (19):4989.
[26]	Osawa T, Fujikawa K, Shimamoto K. 2024. Structures,functions,and syntheses of glycero-glycophospholipids. Frontiers in Chemistry,12:1353688.
[27]	Patel P, Prasad A, Srivastava K, Singh S S, Chakrabarty D, Misra P. 2021. Updates on steroidal alkaloids and glycoalkaloids in Solanum spp.:Biosynthesis,in vitro production and pharmacological values. Studies in Natural Products Chemistry,69:99-127.
[28]	Peng Z, Wang P, Tang D, Shang Y, Li C H, Huang S W, Zhang C Z. 2019. Inheritance of steroidal glycoalkaloids in potato tuber flesh. Journal of Integrative Agriculture, 18 (10):2255-2263. doi: 10.1016/S2095-3119(19)62718-8
[1]	Ahmad D, Zhang Z W, Rasheed H, Xu X Y, Bao J S. 2022. Recent advances in molecular improvement for potato tuber traits. International Journal of Molecular Sciences, 23 (17):9982.
[2]	Akita K, Miyazawa Y. 2023. Auxin biosynthesis,transport,and response directly attenuate hydrotropism in the latter stages to fine-tune root growth direction in Arabidopsis. Physiologia Plantarum, 175 (5):e14051.
[3]	Akiyama R, Umemoto N, Mizutani M. 2023. Recent advances in steroidal glycoalkaloid biosynthesis in the genus Solanum. Plant Biotechnology, 40 (3):185-191. doi: 10.5511/plantbiotechnology.23.0717b pmid: 38293253
[4]	Dhalsamant K, Singh C B, Ravikanth L. 2022. A review on greening and glycoalkaloids in potato tubers:potential solutions. Journal of Agricultural and Food Chemistry, 70 (43):13819-13831.
[5]	Erb M, Kliebenstein D J. 2020. Plant secondary metabolites as defenses,regulators,and primary metabolites:the blurred functional trichotomy. Plant Physiology, 184 (1):39-52.
[6]	Feng J L. 2022. ITRAQ-Based proteomic analysis of the response to Ralstonia solanacearum in potato. Pakistan Journal of Agricultural Sciences, 59 (2):165-171.
[7]	Gan Shengnan. 2023. Evaluation and application of 18 local potato variety resources in Yunnan.[M. D. Dissertation]. Kunming: Yunnan Agricultural University. (in Chinese)
	干胜男. 2023. 云南18份马铃薯地方品种资源的评价与应用[硕士论文]. 昆明: 云南农业大学.
[8]	Gautam H, Sharma A, Trivedi P K. 2023. The role of flavonols in insect resistance and stress response. Current Opinion in Plant Biology,73:102353.
[9]	Guo H, Pu X Q, Jia H, Zhou Y, Ye G J, Yang Y Z, Na T C, Wang J. 2022. Transcriptome analysis reveals multiple effects of nitrogen accumulation and metabolism in the roots,shoots,and leaves of potato(Solanum tuberosum L.). BMC Plant Biology, 22 (1):282.
[10]	Guo Haixia, Zhang Jinjin, An Ran, Qiao Yan, Shi Wenhui, Shi Jing, Zhang Jinwen. 2017. Regulation of SGAs biosynthesis of green tissues in aerial part of the potato. Acta Horticulturae Sinica, 44 (6):1105-1115. (in Chinese) doi: 10.16420/j.issn.0513-353x.2017-0130
[29]	Prysiazhniuk L, Сонець Т Д, Shytikova Y, Melnyk S S, Dikhtiar I, Mazhuha K, Sorochinsky B V. 2023. The environmental and genetic factors affect the productivity and quality of potato cultivars. Zemdirbyste-agriculture, 110 (4):319-328.
[30]	Sah K S, Sofo A. 2024. Editorial:the role of lipids in abiotic stress responses. Frontiers in Plant Science,15:1378485.
[31]	Salam U, Ullah S, Tang Z H, Elateeq A, Khan Y, Khan J, Khan A, Ali S. 2023. Plant metabolomics:an overview of the role of primary and secondary metabolites against different environmental stress factors. Life, 13 (3):706.
[32]	Sang Yueqiu, Yang Qiongfen, Liu Yanhe, Lu Lili, Li Wenjuan, Zhou Jun, Xie Kaiyun, Sui Qijun. 2014. Cultivated regional distribution and year-round production of potato in Yunnan Province. Southwest China Journal of Agricultural Sciences, 27 (3):1003-1008. (in Chinese)
	桑月秋, 杨琼芬, 刘彦和, 卢丽丽, 李文娟, 周俊, 谢开云, 隋启君. 2014. 云南省马铃薯种植区域分布和周年生产. 西南农业学报, 27 (3):1003-1008.
[33]	Sathasivam R, Park S U, Kim J K, Park Y J, Kim M C, Nguyen B V, Lee S Y. 2023. Metabolic profiling of primary and secondary metabolites in Kohlrabi(Brassica oleracea var. gongylodes)sprouts exposed to different light-emitting diodes. Plants, 12 (6):1296.
[34]	Sebastian B, Oliver F, Hans H, Ralph H, Thomas H, Wolfgang E, Corinna D. 2021. Biosynthesis of α-solanine and α-chaconine in potato leaves (Solanum tuberosum L.)-A¹³CO₂ study. Food Chemistry,365:130461.
[35]	Seth T, Asija S, Umar S, Gupta R. 2024. The intricate role of lipids in orchestrating plant defense responses. Plant Science,338:111904.
[10]	郭海霞, 张晶晶, 安然, 乔岩, 石文慧, 石菁, 张金文. 2017. 马铃薯地上部绿色组织中糖苷生物碱合成调控的研究. 园艺学报, 44 (6):1105-1115. doi: 10.16420/j.issn.0513-353x.2017-0130
[11]	Hu W Z, Guan Y Q, Ji Y M, Yang X Z. 2021. Effect of cutting styles on quality,antioxidant activity,membrane lipid peroxidation,and browning in fresh-cut potatoes. Food Bioscience,44:101435.
[12]	Hu X H, Shen S, Wu J L, Liu J, Wang H, He J X, Yao Z L, Bai Y F, Zhang X, Zhu Y, Li G B, Zhao J H, You X M, Xu J, Ji Y P, Li D Q, Pu M, Zhao Z X, Zhou S, Zhang J W, Huang Y, Li Y, Ning Y P, Lu Y L, Huang F, Wang W M, Fan J. 2023. A natural allele of proteasome maturation factor improves rice resistance to multiple pathogens. Nature Plants, 9 (2):228-237.
[13]	Ivanova K, Герасимова C B, Хлесткина E K. 2018. The biosynthesis regulation of potato steroidal glycoalkaloids. Вавиловский журнал генетики и селекции, 22 (1):25-34.
[14]	Jiang T, Du K T, Xie J P, Sun G, Wang P, Chen X, Cao Z M, Wang B C, Chao Q, Li X D, Fan Z F, Zhou T. 2023. Activated malate circulation contributes to the manifestation of light-dependent mosaic symptoms. Cell Reports, 42 (4):112333.
[15]	Koch M, Naumann M, Pawelzik E, Gransee A, Thiel H. 2019. The importance of nutrient management for potato production part I:Plant nutrition and yield. Potato Research, 63 (1):97-119.
[36]	Shen D D, Hua Y P, Huang J Y, Yu S T, Wu T B, Zhang Y N, Li C H, Yue C P. 2021. Multiomic analysis reveals core regulatory mechanisms underlying steroidal glycoalkaloid metabolism in potato tubers. Journal of Agricultural and Food Chemistry, 70 (1):415-426.
[37]	Tilahun S, An H S, Solomon T, Baek M W, Choi H R, Lee H C, Jeong C S. 2020. Indices for the assessment of glycoalkaloids in potato tubers based on surface color and chlorophyll content. Horticulturae, 6 (4):107.
[38]	Wan L B, Gao H D, Gao H L, Du R, Wang F Y, Wang Y, Chen M T. 2022. Selective extraction and determination of steroidal glycoalkaloids in potato tissues by electromembrane extraction combined with LC-MS/MS. Food Chemistry,367:130724.
[39]	Wang Jian, Jiang Fuzhen, Zhou Yun, Zhang Yanping, Shen Haifeng, Pu Xiuqin. 2009. Breeding and cultivation of a new high-quality and drought-resistant potato variety Qingshu No. 9. Bulletin of Agricultural Science and Technology,(2):89-90. (in Chinese)
	王舰, 蒋福祯, 周云, 张艳萍, 申海峰, 蒲秀琴. 2009. 优质抗旱马铃薯新品种青薯9号选育及栽培要点. 农业科技通讯 (2):89-90.
[40]	Wang Xuekui. 2015. Principles and techniques of plant physiological and biochemical experiments. Beijing:Higher Education Press:173-174. (in Chinese)
	王学奎. 2015. 植物生理生化实验原理与技术. 北京:高等教育出版社:173-174.
[41]	Wei Meng, Xu Huizheng, Zhang Ning, Si Huaijun, Tang Xun. 2024. Investigation on current situation of potato processing industry and relevant countermeasure in China. Chinese Potato Journal, 38 (2):176-185. (in Chinese)
	韦孟, 许慧珍, 张宁, 司怀军, 唐勋. 2024. 中国马铃薯加工业现状调查分析及发展对策. 中国马铃薯, 38 (2):176-185.
[42]	Xu G X, Li L J, Zhou J, Lv D G, Zhao D H, Qin S J. 2023. Comparison of transcriptome and metabolome analysis revealed differences in cold resistant metabolic pathways in different apple cultivars under low temperature stress. Horticultural Plant Journal, 9 (2):183-198.
[43]	Xu N, Lu H, Yi X Q, Peng S M, Huang X H, Zhuo Y, He C Z. 2023. Potential of alpha-(α)-solanine as a natural inhibitor of fungus causing leaf spot disease in strawberry. Life, 13 (2):450.
[44]	Xu Ning, Zhang Hongliang, Zhang Ronghua, Xu Yakun. 2021. Current situation and prospect of potato planting in China. Chinese Potato Journal, 35 (1):81-96. (in Chinese)
	徐宁, 张洪亮, 张荣华, 许亚坤. 2021. 中国马铃薯种植业现状与展望. 中国马铃薯, 35 (1):81-96.
[45]	Xu X Y, Vanhercke T, Shrestha P, Luo J X, Akbar S, Konik‐Rose C, Venugoban L, Hussain D, Tian L J, Singh S, Li Z Y, Sharp P J, Liu Q. 2019. Upregulated lipid biosynthesis at the expense of starch production in potato (Solanum tuberosum)vegetative tissues via simultaneous downregulation of ADP-glucose pyrophosphorylase and sugar dependent 1 expressions. Frontiers in Plant Science,10:1444.
[46]	Yan S J, Bhawal R, Yin Z B, Thannhauser T W, Zhang S. 2022. Recent advances in proteomics and metabolomics in plants. Molecular Horticulture, 2 (1):17. doi: 10.1186/s43897-022-00038-9 pmid: 37789425
[47]	Zhang Fengjun, Ye Jingxiu, Wang Yanjun, Wang Jian. 2017. Analysis of differential protein during the different stages leaves of potato drought resistance variety in Qingshu No.9. Seed, 36 (12):70-73,80. (in Chinese)
	张凤军, 叶景秀, 王燕钧, 王舰. 2017. 耐旱马铃薯品种青薯9号不同时期叶片差异蛋白分析. 种子, 36 (12):70-73,80.
[48]	Zhang Tianci, Ge Xiangli, Fu Zhijun, Yu Rong, Chen Yanyun. 2024. Distribution characteristics of solanine content in different organs of potato. China Cucurbits and Vegetables, 37 (2):94-99. (in Chinese)
	张天赐, 葛翔力, 傅致君, 喻荣, 陈彦云. 2024. 马铃薯不同器官中龙葵素含量的分布特征. 中国瓜菜, 37 (2):94-99.
[49]	Zhang X Y, Dong Y L, Lv H Z, Huo Z J, Wang Y N, Li Z H, Liu R X, Wang Z H, Wang Y Q. 2022. Quality diversity and climate impact of nine widely cultivated potato cultivars. Potato Research, 66 (3):597-619.
[50]	Zhao D K, Zhao Y, Chen S Y, Kennelly E J. 2021. Solanum steroidal glycoalkaloids:structural diversity,biological activities,and biosynthesis. Natural Product Reports, 38 (8):1423-1444.
[51]	Zhao Y, Sun R X, Liu H D, Liu X W, Xu K, Xiao K, Zhang S H, Yang X J, Xue C. 2020. Multi-Omics analyses reveal the molecular mechanisms underlying the adaptation of wheat(Triticum aestivum L.)to potassium deprivation. Frontiers in Plant Science,11:588994.
[52]	Zhu T D, Pei H D, Li Z J, Zhang M M, Chen C, Li S Q. 2023. The postharvest application of carvone,abscisic acid,gibberellin,and variable temperature for regulating the dormancy release and sprouting commencement of mini-tuber potato seeds produced under aeroponics. Plants, 12 (23):3952.

类别 Category	可溶性糖/ （mg · g^-1DW） Soluble sugar	还原性总糖/ （mg · g^-1DW） Total reducing sugar	水溶性蛋白质/ （mg · g^-1DW） Water-soluble protein	总淀粉含量/ （% DW） Total starch content	直链淀粉含量/ （%DW） Amylose content	维生素C含量/ （nmol · g^-1FW） Vitamin C content
Q9（S）	4.50 b	1.51 b	3.59 c	19.62 a	7.02 a	673.87 b
Q9（W）	3.86 a	0.70 c	6.49 a	18.81 a	6.27 a	936.83 a
L6（S）	8.31 a	2.66 a	5.38 b	12.16 c	3.92 b	808.02 b
L6（W）	8.26 a	2.06 b	4.12 c	14.12 b	4.71 b	1104.46 a

类别 Category	可溶性糖/ （mg · g^-1DW） Soluble sugar	还原性总糖/ （mg · g^-1DW） Total reducing sugar	水溶性蛋白质/ （mg · g^-1DW） Water-soluble protein	总淀粉含量/ （% DW） Total starch content	直链淀粉含量/ （%DW） Amylose content	维生素C含量/ （nmol · g^-1FW） Vitamin C content
Q9（S）	4.50 b	1.51 b	3.59 c	19.62 a	7.02 a	673.87 b
Q9（W）	3.86 a	0.70 c	6.49 a	18.81 a	6.27 a	936.83 a
L6（S）	8.31 a	2.66 a	5.38 b	12.16 c	3.92 b	808.02 b
L6（W）	8.26 a	2.06 b	4.12 c	14.12 b	4.71 b	1104.46 a

亚细胞定位Subcellular localization	蛋白数量Number of proteins		亚细胞定位Subcellular localization	蛋白数量Number of proteins
亚细胞定位Subcellular localization	Q9-leafW vs. Q9-leafS	Q9-leafW vs. L6-leafW	亚细胞定位Subcellular localization	Q9-leafW vs. Q9-leafS	Q9-leafW vs. L6-leafW
液泡Vacuole	14	13	膜Membrane	94	45
单代膜蛋白Single-pass membrane protein	3	1	多通道膜蛋白Multi-pass membrane protein	79	35
分泌蛋白Secreted protein	27	17	细胞质Cytoplasm	17	8
质体Plastid	48	28	基质侧Stromal side	1	-
细胞核Nucleus	10	11	高尔基体膜Golgi apparatus membrane	2	-
线粒体内膜Mitochondrion inner membrane	3	2	单通道 II 型膜蛋白Single-pass type II membrane protein	1	-
管腔侧Lumenal side	1	1	质体膜Plastid membrane	3	-
脂锚定蛋白Lipid-anchor	2	4	核仁Nucleolus	2	-
膜间隙Intermembrane side	1	2	基质蛋白侧Matrix side	1	-
糖基磷脂酰肌醇锚定蛋白GPI-anchor	2	4	单通道IV 型膜蛋白Single-pass type IV membrane protein	2	-
高尔基体Golgi apparatus	5	2	乙醛酸循环体Glyoxysome	1	-
细胞外空间Extracellular space	8	4	内生体膜Endosome membrane	1	-
内质网膜Extracellular space	2	1	衣被凹陷Coated pit	1	-
内质网Endoplasmic reticulum	1	1	胞质小泡Cytoplasmic vesicle	1	-
胞质Cytosol	2	1	胞质侧Cytoplasmic side	2	-
染色体Chromosome	2	2	细胞质Cytoplasm	17	-
叶绿体类囊体膜Chloroplast thylakoid membrane	27	13	包衣小泡膜COPII-coated vesicle membrane	1	-
叶绿体类囊体管腔Chloroplast Thylakoidlumen	3	3	胞质小泡膜Cytoplasmic vesicle membrane	1	-
叶绿体类囊体Chloroplast thylakoid	1	1	顺面高尔基网Cis-Golgi network	1	-
叶绿体基质Chloroplast stroma	1	1	叶绿体膜Chloroplast membrane	3	-
叶绿体被膜Chloroplast envelope	1	1	叶绿体内膜Chloroplast inner membran	1	-
叶绿体Chloroplast	12	9	细胞膜Cell membrane	7	9
细胞壁Cell wall	10	5	胞间连丝Plasmodesma	-	1
单通道 I 型膜蛋白Single-pass type I membrane protein	-	2	线粒体外模Mitochondrion outer membrane	-	1
质外体Apoplast	7	4	线粒体Mitochondrion	-	1
造粉体Amyloplast	2	2	细胞骨架Cytoskeleton	-	1
过氧物酶体Peroxisome	3	2	细胞连接Cell junction	-	1
外周膜蛋白Peripheral membrane protein	9	6	单通道II型膜蛋白Single-pass type II membrane protein	1	-

亚细胞定位Subcellular localization	蛋白数量Number of proteins		亚细胞定位Subcellular localization	蛋白数量Number of proteins
亚细胞定位Subcellular localization	Q9-leafW vs. Q9-leafS	Q9-leafW vs. L6-leafW	亚细胞定位Subcellular localization	Q9-leafW vs. Q9-leafS	Q9-leafW vs. L6-leafW
液泡Vacuole	14	13	膜Membrane	94	45
单代膜蛋白Single-pass membrane protein	3	1	多通道膜蛋白Multi-pass membrane protein	79	35
分泌蛋白Secreted protein	27	17	细胞质Cytoplasm	17	8
质体Plastid	48	28	基质侧Stromal side	1	-
细胞核Nucleus	10	11	高尔基体膜Golgi apparatus membrane	2	-
线粒体内膜Mitochondrion inner membrane	3	2	单通道 II 型膜蛋白Single-pass type II membrane protein	1	-
管腔侧Lumenal side	1	1	质体膜Plastid membrane	3	-
脂锚定蛋白Lipid-anchor	2	4	核仁Nucleolus	2	-
膜间隙Intermembrane side	1	2	基质蛋白侧Matrix side	1	-
糖基磷脂酰肌醇锚定蛋白GPI-anchor	2	4	单通道IV 型膜蛋白Single-pass type IV membrane protein	2	-
高尔基体Golgi apparatus	5	2	乙醛酸循环体Glyoxysome	1	-
细胞外空间Extracellular space	8	4	内生体膜Endosome membrane	1	-
内质网膜Extracellular space	2	1	衣被凹陷Coated pit	1	-
内质网Endoplasmic reticulum	1	1	胞质小泡Cytoplasmic vesicle	1	-
胞质Cytosol	2	1	胞质侧Cytoplasmic side	2	-
染色体Chromosome	2	2	细胞质Cytoplasm	17	-
叶绿体类囊体膜Chloroplast thylakoid membrane	27	13	包衣小泡膜COPII-coated vesicle membrane	1	-
叶绿体类囊体管腔Chloroplast Thylakoidlumen	3	3	胞质小泡膜Cytoplasmic vesicle membrane	1	-
叶绿体类囊体Chloroplast thylakoid	1	1	顺面高尔基网Cis-Golgi network	1	-
叶绿体基质Chloroplast stroma	1	1	叶绿体膜Chloroplast membrane	3	-
叶绿体被膜Chloroplast envelope	1	1	叶绿体内膜Chloroplast inner membran	1	-
叶绿体Chloroplast	12	9	细胞膜Cell membrane	7	9
细胞壁Cell wall	10	5	胞间连丝Plasmodesma	-	1
单通道 I 型膜蛋白Single-pass type I membrane protein	-	2	线粒体外模Mitochondrion outer membrane	-	1
质外体Apoplast	7	4	线粒体Mitochondrion	-	1
造粉体Amyloplast	2	2	细胞骨架Cytoskeleton	-	1
过氧物酶体Peroxisome	3	2	细胞连接Cell junction	-	1
外周膜蛋白Peripheral membrane protein	9	6	单通道II型膜蛋白Single-pass type II membrane protein	1	-

代谢物的名称与分类The nomenclature and classification of metabolites	表达量倍数差异Fold difference in expression
代谢物的名称与分类The nomenclature and classification of metabolites	Q9-leafW vs. Q9-leafS	Q9-leafW vs. L6-leafW
SGAs生物合成相关的物质（6） Substances related to biosynthesis of SGAs（6）
7-酮基胆固醇 7-Ketocholesterol	0.19	1.24
番茄碱 Tomatidine	8.07	5.98
茄啶 Solanidine	-0.69	-1.47
β-茄碱 beta-Solanine	3.44	4.44
α-茄碱 alpha-Solanine	-5.06	-4.06
α-卡茄碱 alpha-Chaconine	-0.34	-1.03
脂类物质（18） lipid（18）
单硬脂酸甘油酯 Glyceryl monostearate	-1.60	-
2-棕榈酰甘油 2-Palmitoylglycerol	-1.29	-
(1R,2S,4R,5R)-5-羟基-1,7,7-三甲基双环[2.2.1]庚-2-基6-O-[(2R,3R,4R)-四氢-3,4-二羟基-4-(羟甲基)-2-呋喃基]-β-D-吡喃葡萄糖苷 (1R,2S,4R,5R)-5-Hydroxy-1,7,7-trimethylbicyclo[2.2.1]hept-2-yl6-O-[（2R,3R,4R）-tetrahydro-3,4-dihydroxy-4-(hydroxymethyl)-2-furanyl]-β-D-glucopyranoside	-2.84	-
4,4,8,10,14-五甲基-17-(4,5,6-三羟基-6-甲基庚烷-2-基)-2，5，6，7，9，15-六氢-1H-环戊二烯[a]菲-3,16-二酮 4，4，8，10，14-Pentamethyl-17-(4,5,6-trihydroxy-6-methylheptan-2-yl)-2,5,6,7,9,15-hexahydro-1H-cyclopenta[a]phenanthrene-3，16-dione	-2.74	-
芥酸酰胺 Erucamide	-2.18	-
叶黄素 Lutein	-1.40	-
贝母甲素 Peimine	-	-1.18
3,5,5-三甲基-4-[3-[(6-O-β-D-木吡喃糖基-β-D-吡喃葡萄糖基)氧]丁基]-2-环己烯-1-酮 3,5,5-Trimethyl-4-[3-[(6-O-β-D-xylopyranosyl-β-D-glucopyranosyl)oxy]butyl]-2-cyclohexen-1-one	-	1.33
9S-羟基-10E,12Z,15Z-十八碳三烯酸 9-HOTrE	3.43	-
十八碳四烯酸 FA 18︰4	3.17	-
9,12,13-三羟基十八碳-10,15-二烯酸9,12,13-Trihydroxyoctadeca-10,15-dienoic acid	1.32	-
乳糖醇 Lactitol	4.40	-
脂肪酸 Sebacic acid	3.47	-
16-羟基棕榈酸 FA 16︰0；2O	4.49	-
(S)-2-羟基-2-甲基琥珀酸 (S)-2-Hydroxy-2-methylsuccinic acid	-	3.17
12,13-二羟基十八烷基-9-烯酸 12,13-Dihydroxyoctadec-9-enoic acid	-	2.26
(Z)-5,8,11-三羟基十八烷基-9-烯酸 (Z)-5,8,11-Trihydroxyoctadec-9-enoic acid	-	1.24
十八碳二烯四氧酸 FA 18：2；4O	-	-1.00
有机氧化物（11） Organic oxide（11）
蔗糖 Sucrose	-	-1.69
苯乙基-2-O-(β-D-吡喃木糖基)-β-D-吡喃葡萄糖苷Phenethyl2-O-(β-D-xylopyranosyl)-β-D-glucopyranoside	-	-1.22
苯甲醇 + 己烷 Benzyl alcohol + Hex-Pen	-1.84	-
决奈达隆 Dronedarone	3.86	-
苯甲醇 + 己烷 Benzyl alcohol + Hex-Hex	-2.00	-
(2S,3S,4S,5R,6R）-6-(3-苯甲酰氧基-2-羟基丙氧基)-3,4,5-三羟基氧烷-2-羧酸 (2S,3S,4S,5R,6R）-6-(3-benzoyloxy-2-hydroxypropoxy）-3,4,5-trihydroxyoxane-2-carboxylic acid	-	3.97
3-O-阿魏酰奎尼酸 3-O-Feruloylquinic acid	-	1.58
红景天苷 Salidroside	-	3.16
山梨醇 Sorbo	-	4.64
乳酮糖 Lactulose	-	-1.79
红霉素 Erythromycin	-	-2.18
苯丙类和聚酮类物质（9） Phenylpropyl and polyketone substances（9）
8,8-二甲基-2-苯基-4H，8-H-苯并[1，2-b：3,4-b’]二吡喃-4-酮 8,8-Dimethyl-2-phenyl-4H，8H-benzo[1，2-b：3,4-b’]dipyran-4-one	1.60	-
黄芩苷II Scutellarioside II	-1.74	-
山奈酚 Kaempferol	-3.64	-
阿魏酰-O-甲氧基酪胺 Feruloyl O-methyldopamine	4.21	-
(S)-7-(2-O-6-脱氧-α-L-甘露糖基)-β-D-吡喃葡萄糖基)-2,3-二氢-5-羟基-2-(3-羟基-4-甲氧基苯基)-4H-1-苯并吡喃-4-酮 (S)-7-(2-O-6-Deoxy-α-L-mannopyranosyl)-β-D-glucopyranosyl) oxy)-2,3-dihydro-5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4H-1-benzopyran-4-one	-	-1.15
3-（4-羟基-3-甲氧基苯基）-2-丙烯酸 3-（4-Hydroxy-3-methoxyphenyl）-2-propenoic acid	-	4.45
芦丁 Rutin	-	1.58
（+/-）-木脂酰胺（+/-）-Grossamide	-	3.62
秦皮甲素 Esculin	-	-1.12
有机杂环化合物（6） Organic Heterocyclic Compounds（6）
（3R,4R,5R）-3-[（11E）-11,15-十六碳二烯-9-炔-1-基]-4-羟基-5-甲基二氢-2（3H）-呋喃酮（3R,4R,5R）-3-[（11E）-11，15-Hexadecadien-9-yn-1-yl]-4-hydroxy-5-methyldihydro-2（3H）-furanone	-3.32	-
培高利特 Pergolide	0.88	-
14,15-脱氧喜沃林 Deoxykhivorin	1.72	-
3-[(7S,8R)-5-羟基-2,2,7,8-四甲基-6-氧代-7,8-二氢吡喃[3,2-g]铬-10-基]-3-苯基丙酸 3-[(7S,8R)-5-Hydroxy-2,2,7,8-tetramethyl-6-oxo-7,8-dihydropyrano[3,2-g]chromen-10-yl]-3-phenylpropanoic acid	-2.56	-
脱镁叶绿甲酯酸A Pheophorbide A	2.58	-
升麻素苷 prim-O-b-D-Glucosylcimifugin	-	3.27
有机酸及其衍生物（3） Organic acids and their derivatives（3）
1-氨基-2-羧基吡咯烷 Pyrrolidin-1-ium-2-carboxylate	3.92	-
波纳丁 Bonactin	-	1.94
氢离子；2-羟基丙烷-1,2,3-三羧酸盐 Hydron；2-hydroxypropane-1,2,3-tricarboxylate	-2.12	-
苯环型化合物（1） Benzene ring compound（1）
甜菜安 Desmedipham	-	2.18
核苷、核苷酸及其类似物（1） Nucleosides，nucleotides and their analogs（1）
阿糖尿苷 Arabinofuranosyluracil	2.79	-

Understanding the Anabolic Differences of Steroidal Glycoalkaloids in Leaf Between Two Potato Varieties Grown at the Winter and Spring Cropping Patterns

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 52

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YANG Li, QIN Ya, BIAN Baciren, ZHEN Zhen, QIONG Ji, BAI Maquzhen, ZHOU Ya. A New Cultivar of Colored Potato‘Xigezi 2’ [J]. Acta Horticulturae Sinica, 2025, 52(S1): 145-146.
[2]	LÜ Wentang, YIN Jingjing, ZHANG Jian, XU Guoxin, JIANG Xu, YU Yang, WU Xiu, LI Xiaozun. A New Cultivar of Ornamental Lotus‘Bingjian Heyun’ [J]. Acta Horticulturae Sinica, 2025, 52(S1): 209-210.
[3]	DOU Xueting, ZHU Xi, ZHANG Ning, and SI Huaijun. Functional Analysis of StHY5 Associated with Low-Temperature Stress in Potato [J]. Acta Horticulturae Sinica, 2025, 52(7): 1745-1757.
[4]	SONG Qianna, SONG Lushuai, DUAN Yonghong, BAI Xiaodong, and FENG Ruiyun. Establishment a System to Obtain CRISPR/Cas9 Genome Editing Plants Based on Hairy Root Transformation in Potatoes [J]. Acta Horticulturae Sinica, 2025, 52(7): 1758-1768.
[5]	YU Cannan, GUO Anfang, LU Chun, MIAO Hongjun, HOU Xiaoyu, YUAN Tianyi, LIU Guoyuan, WEI Hui, ZHANG Jian, and YU Chunmei. Identification and Development of SNP Markers for Lagerstroemia indica Leaf Color Based on Transcriptome and Genome Analysis [J]. Acta Horticulturae Sinica, 2025, 52(7): 1803-1816.
[6]	YE Yanran, LIU Jiangang, BIAN Chunsong, GUO Huachun, and JIN Liping. Quantitative Analysis of Potato Growth Under Different Nitrogen Management Practices Based on UAV-Acquired RGB Imagery [J]. Acta Horticulturae Sinica, 2025, 52(7): 1870-1882.
[7]	LI Ziyan, CHEN Weixi, LI Zihan, LI Yin, LIANG Fengming, ZENG Xiangli, JIAN Hongju, and LÜ Dianqiu. Screening Candidate Genes Controlling Potato Maturation Time Based on RNA-Seq [J]. Acta Horticulturae Sinica, 2025, 52(6): 1505-1518.
[8]	LIU Yanan, BAO Dandan, ZHANG Sipu, NIU Jiajia, XU Zhifei, YANG Yongfeng, and LU Yunfeng. Effects of Foliar Spraying of Nano-selenium on Amino Acid Content and Metabolome of Kiwifruit [J]. Acta Horticulturae Sinica, 2025, 52(6): 1575-1587.
[9]	SHA Tongyun, LI Zhangping, HU Zhongyuan, ZHANG Mingfang, YANG Jinghua. Functional Genomics and Molecular Breeding in Brassica juncea [J]. Acta Horticulturae Sinica, 2025, 52(5): 1189-1212.
[10]	ZHENG Jingyi, LI Xiaonan, PIAO Zhongyun. Multi-omics Approaches to Improve the Studies on the Mechanisms of Clubroot Resistance in Brassica Hosts [J]. Acta Horticulturae Sinica, 2025, 52(5): 1213-1232.
[11]	CHU Wenlong, ZHANG Xiaoli, XU Liang, WANG Yan, LIU Liwang. Advances in Genomics and Molecular Breeding in Radish [J]. Acta Horticulturae Sinica, 2025, 52(5): 1251-1270.
[12]	ZHANG Yiying, ZHANG Yu, CHU Yunxia, CHEN Hairong, DENG Shan, LI Aiai, ZHAO Hong, LIU Kun, HAN Ruixi, REN Li. Color Characteristics of Broccoli and Cauliflower Leaves Based on the Colorimeter Parameters [J]. Acta Horticulturae Sinica, 2025, 52(4): 872-882.
[13]	KONG Jiatao, FU Caixia, WU Yanuo, LIU Yuan, HU Zhehui, XU Juan, HUANG Hao, ZHAO Zhao, CHEN Lei, CHEN Jiajing. Flavoromics Analysis and Flavor Quality Difference Analysis in Bingtang Oranges [J]. Acta Horticulturae Sinica, 2025, 52(4): 984-996.
[14]	LIN Xi, DENG Zhenpeng, YANG Xinyue, ZHOU Keyou, YI Xiaoping, WANG Jichun. Effects of Reducing Chemical Fertilizer Combined with Organic Fertilizer on Potato Yield and Nutrient Utilization [J]. Acta Horticulturae Sinica, 2025, 52(4): 1007-1019.
[15]	WANG Jie, DONG Xiaoxu, CHEN Jinxiao, YU Xianmei, GAO Rui, AI Chengxiang, SHEN Guangning. Identification and Biological Characteristics of the Pathogen Causing Persimmon Leaf Blight and Toxicity Test of Different Fungicides [J]. Acta Horticulturae Sinica, 2025, 52(3): 737-748.