Construction of Transient Transformation System of
Petals in Prunus mume

doi:10.16420/j.issn.0513-353x.2024-0448

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (4): 908-920.doi: 10.16420/j.issn.0513-353x.2024-0448

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

Construction of Transient Transformation System of Petals in Prunus mume

TIAN Zhuang，FANG Chenxi，LIU Yating，WANG Yutong，XUE Linlei，SHI Jiaxin，REN Tianfang，ZHANG Junwei，BAO Manzhu，and ZHANG Jie^*(flybebrave@mail.hzau.edu.cn)

National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops，College of Horticulture and Forestry Sciences，Huazhong Agricultural University，Wuhan 430070，China

Received:2024-12-25 Revised:2025-01-24 Online:2025-04-25 Published:2025-04-25
Contact: ZHANG Jie

Abstract

Abstract: This study takes the petals of Prunus mume as the receptor and GUS gene as the reporter gene. The influences of 7 main factors on the transient transformation efficiency of Prunus mume petals were conducted through an orthogonal design and single factor analysis method. The effects of infection mode，infection time，development stage and genotype on the transient transformation efficiency of Prunus mume petals were investigated by single factor experiment. The results showed that the whole flower vacuum method，infestation time of 15 min，development stage of initial blooming，and‘XueMei’ genotype had the highest transient expression rate of GUS gene，respectively. The orthogonal experiment was further designed to explore the effect of the combination of Agrobacterium strain type，OD₆₀₀ value，Coculture time on the transient transformation efficiency of Prunus mume petals. The results showed that the three factors affected GUS transient expression in the order of coculture time，Agrobacterium strains type，OD₆₀₀ value. Among them，the combination of Agrobacterium EHA105，OD₆₀₀values is 0.6 and coculture time is 5 d showed the highest GUS transient expression effect of Prunus mume petals（32.67%）. Then，the optimal combination screened was verified by applying an anthocyanin synthesis gene（PmANS）as the target gene.

Key words: Prunus mume, petal, Agrobacterium-mediated method, transient transformation, GUS

CLC Number:

S 685.17

TIAN Zhuang, FANG Chenxi, LIU Yating, WANG Yutong, XUE Linlei, SHI Jiaxin, REN Tianfang, ZHANG Junwei, BAO Manzhu, ZHANG Jie. Construction of Transient Transformation System of Petals in Prunus mume[J]. Acta Horticulturae Sinica, 2025, 52(4): 908-920.

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Construction of Transient Transformation System of Petals in Prunus mume

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