Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (4): 897-907.doi: 10.16420/j.issn.0513-353x.2024-0471
• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles
WANG Jin1, GAO Li2, HE Hongyu3, TU Xunliang1, JIANG Yu1,*()
Received:
2025-02-05
Revised:
2025-03-17
Online:
2025-05-08
Published:
2025-04-25
Contact:
JIANG Yu
WANG Jin, GAO Li, HE Hongyu, TU Xunliang, JIANG Yu. Characteristic Analysis of Aroma Components in Interspecific Hybrids of Cymbidium[J]. Acta Horticulturae Sinica, 2025, 52(4): 897-907.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2024-0471
样本 Sample | 形态指标Morphological indices | ||||
---|---|---|---|---|---|
一箭着花数 Number of inflorescence | 花莛颜色 Scape color | 花色 Flower color | 舌色 Tongue color | 花瓣是否有红色条纹Whether the petals have red stripes | |
墨兰‘翠玉’ Cymbidium sinense‘Cuiyu’ | 6 ± 2 a | 青红 Cyanine red | 紫红色 Violet red | 长舌,外卷,黄色,有红斑点、斑块 Long tongue,curling outward,yellow,with red spots and plaques | 是 Yes |
春兰‘宋梅’ C. georingii‘Songmei’ | 1 b | 绿 Green | 绿 Green | 圆舌,白色,有红斑点 Round tongue,white,with red spots | 否 No |
杂交兰O1 Hybrid orchid O1 | 3 ± 1 b | 红 Red | 红 Red | 长舌,外卷,黄色,有红斑点 Long tongue,curling outward,yellow,with red spots | 是 Yes |
杂交兰O2 Hybrid orchid O2 | 2 ± 1 b | 红 Red | 红 Red | 圆长舌,外卷,黄色,有红色斑块 Long round tongue,curling outward,yellow,with red plaques | 是 Yes |
Table 1 Morphological characteristics of Cymbidium georingii‘Songmei’,Cymbidium sinense‘Cuiyu’and their hybrids
样本 Sample | 形态指标Morphological indices | ||||
---|---|---|---|---|---|
一箭着花数 Number of inflorescence | 花莛颜色 Scape color | 花色 Flower color | 舌色 Tongue color | 花瓣是否有红色条纹Whether the petals have red stripes | |
墨兰‘翠玉’ Cymbidium sinense‘Cuiyu’ | 6 ± 2 a | 青红 Cyanine red | 紫红色 Violet red | 长舌,外卷,黄色,有红斑点、斑块 Long tongue,curling outward,yellow,with red spots and plaques | 是 Yes |
春兰‘宋梅’ C. georingii‘Songmei’ | 1 b | 绿 Green | 绿 Green | 圆舌,白色,有红斑点 Round tongue,white,with red spots | 否 No |
杂交兰O1 Hybrid orchid O1 | 3 ± 1 b | 红 Red | 红 Red | 长舌,外卷,黄色,有红斑点 Long tongue,curling outward,yellow,with red spots | 是 Yes |
杂交兰O2 Hybrid orchid O2 | 2 ± 1 b | 红 Red | 红 Red | 圆长舌,外卷,黄色,有红色斑块 Long round tongue,curling outward,yellow,with red plaques | 是 Yes |
Fig. 1 Morphological metrics of Cymbidium georingii‘Songmei’,Cymbidium sinense‘Cuiyu’and their hybrids Different lowercase letters indicate significant differences(P < 0.05)among different samples
样本 Sample | 相对含量/% Relative content | |||||||
---|---|---|---|---|---|---|---|---|
萜烯类 Terpenoids | 醇类 Alcohols | 酯类 Esters | 醛类 Aldehydes | 酮类 Ketones | 酸类 Acids | 烷烃类 Alkanes | 其他 Others | |
墨兰‘翠玉’Cymbidium sinense‘Cuiyu’ | 24.37 | 12.94 | 4.99 | — | 18.98 | 0.28 | 15.92 | 22.52 |
春兰‘宋梅’C. georingii‘Songmei’ | 14.08 | 65.20 | 3.18 | — | 2.41 | 0.82 | 3.09 | 11.23 |
杂交兰O1 Hybrid orchid O1 | 8.28 | 28.98 | — | 41.83 | 17.71 | — | 0.67 | 2.52 |
杂交兰O2 Hybrid orchid O2 | 28.11 | 49.59 | — | 1.16 | 10.44 | — | 4.76 | 5.96 |
Table 2 The relative content of floral volatile compounds in Cymbidium georingii‘Songmei’,Cymbidium sinense‘Cuiyu’and their hybrid
样本 Sample | 相对含量/% Relative content | |||||||
---|---|---|---|---|---|---|---|---|
萜烯类 Terpenoids | 醇类 Alcohols | 酯类 Esters | 醛类 Aldehydes | 酮类 Ketones | 酸类 Acids | 烷烃类 Alkanes | 其他 Others | |
墨兰‘翠玉’Cymbidium sinense‘Cuiyu’ | 24.37 | 12.94 | 4.99 | — | 18.98 | 0.28 | 15.92 | 22.52 |
春兰‘宋梅’C. georingii‘Songmei’ | 14.08 | 65.20 | 3.18 | — | 2.41 | 0.82 | 3.09 | 11.23 |
杂交兰O1 Hybrid orchid O1 | 8.28 | 28.98 | — | 41.83 | 17.71 | — | 0.67 | 2.52 |
杂交兰O2 Hybrid orchid O2 | 28.11 | 49.59 | — | 1.16 | 10.44 | — | 4.76 | 5.96 |
Fig. 2 Score diagram and load diagram of principal component analysis for volatile component in Cymbidium georingii ‘Songmei’,Cymbidium sinense‘Cuiyu’and their hybrid
挥发性成分 Volatile components | 相对含量/% Relative content | ||||
---|---|---|---|---|---|
分类 Classification | 组分 Components | 墨兰‘翠玉’ Cymbidium sinens ‘Cuiyu’ | 春兰‘宋梅’ C. georingi ‘Songmei’ | 杂交兰O1 Hybrid orchid O1 | 杂交兰O2 Hybrid orchid O2 |
萜烯类 Terpenoids | α-蒎烯α-Pinene | 7.61 ± 0.98 | 3.14 ± 0.34 | — | 4.13 ± 0.45 |
柠檬烯D-Limonene | 7.81 ± 1.21 | — | 4.59 ± 0.98 | — | |
罗勒烯 (E)-β-ocimene | — | — | — | 11.44 ± 2.21 | |
3-蒈烯 3-Carene | — | — | — | 4.56 ± 0.09 | |
4,8-二甲基-1,7-壬二烯 4,8-Dimethyl-1,7-nonadiene | 8.95 ± 0.35 | — | — | — | |
大柱头-4,6(E),8(Z)-三 (6E,8Z)-Megastigma-4,6,8-triene | — | — | — | 3.30 ± 0.19 | |
石竹烯 β-Caryophyllene | — | 10.94 ± 1.71 | — | 2.99 ± 0.55 | |
α-香柠檬烯 α-Bergamotene | — | — | — | 0.44 ± 0.09 | |
胡萝卜烯 Daucene | — | — | 3.69 ± 0.28 | 1.25 ± 0.08 | |
醇类 Alcohols | β-二氢香芹醇(1S,2R,4S)-Neo-dihydrocarveol | 12.94 ± 1.54 | — | — | 2.94 ± 0.11 |
6-甲基-5-庚烯-2-醇 6-Merhyl-5-hepten-2-ol | — | — | 15.98 ± 2.63 | 34.73 ± 4.37 | |
苯甲醇 Benzyl alcohol | — | 58.54 ± 6.53 | — | — | |
苯乙醇 Phenethyl alcohol | — | 3.74 ± 0.60 | — | — | |
桉叶油醇 Eucalyptol | — | — | 13.00 ± 1.24 | — | |
2,7-二甲基-2,6-辛二烯-1-醇 2,6-Octadien-1-ol, 2,7-dimethyl- | — | — | — | 5.19 ± 0.29 | |
α-松油醇 α-Terpineol | — | 0.35 ± 0.05 | — | — | |
木焦油醇 2-Methoxy-4-methylphenol | — | 2.57 ± 0.37 | — | 1.97 ± 0.35 | |
2-乙烯基-2,5-二甲基-4-己烯-1-醇 2-Ethenyl-2,5-dimethyl-4-hexen-1-ol | — | — | — | 2.61 ± 0.19 | |
4-萜烯醇 Terpinen-4-ol | — | — | — | 2.15 ± 0.32 | |
酯类Esters | 己酸乙酯 Ethyl hexanoate | 4.99 ± 0.55 | — | — | — |
亚硝酸仲丁酯 sec-Butyl nitrite | — | 3.18 ± 0.52 | — | — | |
醛类Aldehyde | 异戊醛 3-Methylbutanal | — | — | 20.88 ± 3.89 | — |
正庚醛 Heptanal | — | — | 0.75 ± 0.06 | 1.16 ± 0.17 | |
己醛 Hexanal | — | — | 14.30 ± 1.45 | — | |
6,6-二甲基双环[3.1.1]庚烷-2-甲醛; 6,6-Dimethylbicyclo(3.1.1)heptane-2-carbaldehyde | — | — | 5.90 ± 0.46 | — | |
酮类Ketone | 侧柏酮 α-Thujene | — | — | — | 4.38 ± 0.59 |
甲基庚烯酮 6-Methyl-5-hepten-2-one | 18.98 ± 1.56 | 2.41 ± 0.45 | 17.71 ± 2.01 | 6.06 ± 1.03 | |
酸类Acids | Z-L-精氨酸 Nalpha-Cbz-L-Arginine | 0.28 ± 0.05 | 0.82 ± 0.08 | — | — |
烷烃类Alkanes | rel-(2R*,3S*))-2,3-环氧庚烷rel-(2R*,3S*)-2,3-Epoxyheptane | — | 3.09 ± 0.29 | — | — |
(1-甲基丁基)-环氧乙烷 2-pentan-2-yloxirane | 15.92 ± 2.06 | — | — | 4.50 ± 0.79 | |
(±)-茶螺烷 Theaspirane | — | — | 0.67 ± 0.07 | 0.26 ± 0.07 | |
其他Others | 对甲基苯甲醚 4-Methylanisole | — | 8.95 ± 1.58 | — | — |
(1R,2R,3R,5S)-3-蒎烷胺 (-)-Isopinocampheylamine | 8.48 ± 0.79 | — | — | 1.90 ± 0.51 | |
2,4,6-三氯苯甲醚 2,4,6-Trichloroanisole | — | — | 2.52 ± 0.23 | 1.72 ± 0.31 | |
3,4-二甲氧基甲苯 3,4-Dimethoxytoluene | — | — | — | 2.34 ± 0.25 | |
对甲酚 4-Methylphenol | 9.73 ± 1.03 | — | — | — | |
对苯二甲醚 1,4-Dimethoxybenzene | — | 2.28 ± 0.33 | — | — | |
2-甲氧基-3-甲基苯酚 2-Methoxy-3-methylphenol | 4.31 ± 0.63 | — | — | — |
Table 3 Volatile components types and contents of Cymbidium georingii‘Songmei’,Cymbidium sinense‘Cuiyu’and their hybrid
挥发性成分 Volatile components | 相对含量/% Relative content | ||||
---|---|---|---|---|---|
分类 Classification | 组分 Components | 墨兰‘翠玉’ Cymbidium sinens ‘Cuiyu’ | 春兰‘宋梅’ C. georingi ‘Songmei’ | 杂交兰O1 Hybrid orchid O1 | 杂交兰O2 Hybrid orchid O2 |
萜烯类 Terpenoids | α-蒎烯α-Pinene | 7.61 ± 0.98 | 3.14 ± 0.34 | — | 4.13 ± 0.45 |
柠檬烯D-Limonene | 7.81 ± 1.21 | — | 4.59 ± 0.98 | — | |
罗勒烯 (E)-β-ocimene | — | — | — | 11.44 ± 2.21 | |
3-蒈烯 3-Carene | — | — | — | 4.56 ± 0.09 | |
4,8-二甲基-1,7-壬二烯 4,8-Dimethyl-1,7-nonadiene | 8.95 ± 0.35 | — | — | — | |
大柱头-4,6(E),8(Z)-三 (6E,8Z)-Megastigma-4,6,8-triene | — | — | — | 3.30 ± 0.19 | |
石竹烯 β-Caryophyllene | — | 10.94 ± 1.71 | — | 2.99 ± 0.55 | |
α-香柠檬烯 α-Bergamotene | — | — | — | 0.44 ± 0.09 | |
胡萝卜烯 Daucene | — | — | 3.69 ± 0.28 | 1.25 ± 0.08 | |
醇类 Alcohols | β-二氢香芹醇(1S,2R,4S)-Neo-dihydrocarveol | 12.94 ± 1.54 | — | — | 2.94 ± 0.11 |
6-甲基-5-庚烯-2-醇 6-Merhyl-5-hepten-2-ol | — | — | 15.98 ± 2.63 | 34.73 ± 4.37 | |
苯甲醇 Benzyl alcohol | — | 58.54 ± 6.53 | — | — | |
苯乙醇 Phenethyl alcohol | — | 3.74 ± 0.60 | — | — | |
桉叶油醇 Eucalyptol | — | — | 13.00 ± 1.24 | — | |
2,7-二甲基-2,6-辛二烯-1-醇 2,6-Octadien-1-ol, 2,7-dimethyl- | — | — | — | 5.19 ± 0.29 | |
α-松油醇 α-Terpineol | — | 0.35 ± 0.05 | — | — | |
木焦油醇 2-Methoxy-4-methylphenol | — | 2.57 ± 0.37 | — | 1.97 ± 0.35 | |
2-乙烯基-2,5-二甲基-4-己烯-1-醇 2-Ethenyl-2,5-dimethyl-4-hexen-1-ol | — | — | — | 2.61 ± 0.19 | |
4-萜烯醇 Terpinen-4-ol | — | — | — | 2.15 ± 0.32 | |
酯类Esters | 己酸乙酯 Ethyl hexanoate | 4.99 ± 0.55 | — | — | — |
亚硝酸仲丁酯 sec-Butyl nitrite | — | 3.18 ± 0.52 | — | — | |
醛类Aldehyde | 异戊醛 3-Methylbutanal | — | — | 20.88 ± 3.89 | — |
正庚醛 Heptanal | — | — | 0.75 ± 0.06 | 1.16 ± 0.17 | |
己醛 Hexanal | — | — | 14.30 ± 1.45 | — | |
6,6-二甲基双环[3.1.1]庚烷-2-甲醛; 6,6-Dimethylbicyclo(3.1.1)heptane-2-carbaldehyde | — | — | 5.90 ± 0.46 | — | |
酮类Ketone | 侧柏酮 α-Thujene | — | — | — | 4.38 ± 0.59 |
甲基庚烯酮 6-Methyl-5-hepten-2-one | 18.98 ± 1.56 | 2.41 ± 0.45 | 17.71 ± 2.01 | 6.06 ± 1.03 | |
酸类Acids | Z-L-精氨酸 Nalpha-Cbz-L-Arginine | 0.28 ± 0.05 | 0.82 ± 0.08 | — | — |
烷烃类Alkanes | rel-(2R*,3S*))-2,3-环氧庚烷rel-(2R*,3S*)-2,3-Epoxyheptane | — | 3.09 ± 0.29 | — | — |
(1-甲基丁基)-环氧乙烷 2-pentan-2-yloxirane | 15.92 ± 2.06 | — | — | 4.50 ± 0.79 | |
(±)-茶螺烷 Theaspirane | — | — | 0.67 ± 0.07 | 0.26 ± 0.07 | |
其他Others | 对甲基苯甲醚 4-Methylanisole | — | 8.95 ± 1.58 | — | — |
(1R,2R,3R,5S)-3-蒎烷胺 (-)-Isopinocampheylamine | 8.48 ± 0.79 | — | — | 1.90 ± 0.51 | |
2,4,6-三氯苯甲醚 2,4,6-Trichloroanisole | — | — | 2.52 ± 0.23 | 1.72 ± 0.31 | |
3,4-二甲氧基甲苯 3,4-Dimethoxytoluene | — | — | — | 2.34 ± 0.25 | |
对甲酚 4-Methylphenol | 9.73 ± 1.03 | — | — | — | |
对苯二甲醚 1,4-Dimethoxybenzene | — | 2.28 ± 0.33 | — | — | |
2-甲氧基-3-甲基苯酚 2-Methoxy-3-methylphenol | 4.31 ± 0.63 | — | — | — |
Fig. 3 The comparison of common volatile component in Cymbidium georingii‘Songmei’,Cymbidium sinense‘Cuiyu’and their hybrid * indicates significant differences among different samples at 0.05 level
挥发性成分 Volatile component | CAS号 CAS number | 香味描述 Aroma description | 香味类型 Odor type |
---|---|---|---|
α-蒎烯 α-pinene | 7785-70-8 | 薄荷味 Minty | 草药香型 Herbal |
柠檬烯 D-Limonene | 5989-27-5 | 柑橘味,甜味Citrusy,sweet | 果香型 Fruity |
罗勒烯 (E)-β-ocimene | 13877-91-3 | 柑橘味Citrusy | 花香型 Floral |
3-蒈烯 3-Carene | 13466-78-9 | 药用木香 Medicinal woody | 木香型 Woody |
石竹烯 β-Caryophyllene | 87-44-5 | 丁香味 Clove-like | 香料型 Spicy |
6-甲基-5-庚烯-2-醇6-Merhyl-5-hepten-2-ol | 1569-60-4 | 香菜味 Parsley-like | 清香型Green |
苯甲醇 Benzyl alcohol | 100-51-6 | 温和花香 Gentle floral | 花香型 Floral |
苯乙醇 Phenethyl alcohol | 60-12-8 | 玫瑰花香 Rose-like | 花香型 Floral |
桉叶油醇 Eucalyptol | 470-82-6 | 桉树、草药香 Eucalyptus-like,herbal | 草药香型 Herbal |
α-松油醇 α-Terpineol | 98-55-5 | 紫丁香味 Lilac-like | 花香型 Floral |
木焦油醇 2-Methoxy-4-methylphenol | 93-51-6 | 辛辣味、皮革味 Pungent,leather-like | 香料型 Spicy |
4-萜烯醇 Terpinen-4-ol | 562-74-3 | 胡椒木质混合味Peppery mixed with woody | 香料型 Spicy |
己酸乙酯 Ethyl Hexanoate | 123-66-0 | 甜味,果味 Sweet,fruity | 果香型 Fruity |
异戊醛 3-Methylbutanal | 590-86-3 | 桃子味 Peach-like | 果香型 Fruity |
正庚醛 Heptanal | 111-71-7 | 脂蜡气味 Wax-like | 果香型 Fruity |
己醛 Hexanal | 66-25-1 | 青草香 Grass-like | 清香型 Green |
侧柏酮 α-Thujene | 2867-05-2 | 木质气味 Woody | 木香型 Woody |
甲基庚烯酮 6-Methyl-5-hepten-2-one | 110-93-0 | 柑橘味 Citrusy | 果香型 Fruity |
(±)-茶螺烷 Theaspirane | 36431-72-8 | 辛辣木质香 Spicy woody | 草药香型 Herbal |
对甲酚 4-Methylphenol | 106-44-5 | 酚类芳香味 Phenolic | 化学型 Chemical |
对甲基苯甲醚 4-Methylanisole | 104-93-8 | 坚果味 Nutty | 化学型 Chemical |
对苯二甲醚 1,4-Dimethoxybenzene | 150-78-7 | 甜味,茴香味Sweet,fennel | 清香型 Green |
Table 4 Aroma type characteristics of volatile component in Cymbidium georingii‘Songmei’,Cymbidium sinense‘Cuiyu’and their hybrid
挥发性成分 Volatile component | CAS号 CAS number | 香味描述 Aroma description | 香味类型 Odor type |
---|---|---|---|
α-蒎烯 α-pinene | 7785-70-8 | 薄荷味 Minty | 草药香型 Herbal |
柠檬烯 D-Limonene | 5989-27-5 | 柑橘味,甜味Citrusy,sweet | 果香型 Fruity |
罗勒烯 (E)-β-ocimene | 13877-91-3 | 柑橘味Citrusy | 花香型 Floral |
3-蒈烯 3-Carene | 13466-78-9 | 药用木香 Medicinal woody | 木香型 Woody |
石竹烯 β-Caryophyllene | 87-44-5 | 丁香味 Clove-like | 香料型 Spicy |
6-甲基-5-庚烯-2-醇6-Merhyl-5-hepten-2-ol | 1569-60-4 | 香菜味 Parsley-like | 清香型Green |
苯甲醇 Benzyl alcohol | 100-51-6 | 温和花香 Gentle floral | 花香型 Floral |
苯乙醇 Phenethyl alcohol | 60-12-8 | 玫瑰花香 Rose-like | 花香型 Floral |
桉叶油醇 Eucalyptol | 470-82-6 | 桉树、草药香 Eucalyptus-like,herbal | 草药香型 Herbal |
α-松油醇 α-Terpineol | 98-55-5 | 紫丁香味 Lilac-like | 花香型 Floral |
木焦油醇 2-Methoxy-4-methylphenol | 93-51-6 | 辛辣味、皮革味 Pungent,leather-like | 香料型 Spicy |
4-萜烯醇 Terpinen-4-ol | 562-74-3 | 胡椒木质混合味Peppery mixed with woody | 香料型 Spicy |
己酸乙酯 Ethyl Hexanoate | 123-66-0 | 甜味,果味 Sweet,fruity | 果香型 Fruity |
异戊醛 3-Methylbutanal | 590-86-3 | 桃子味 Peach-like | 果香型 Fruity |
正庚醛 Heptanal | 111-71-7 | 脂蜡气味 Wax-like | 果香型 Fruity |
己醛 Hexanal | 66-25-1 | 青草香 Grass-like | 清香型 Green |
侧柏酮 α-Thujene | 2867-05-2 | 木质气味 Woody | 木香型 Woody |
甲基庚烯酮 6-Methyl-5-hepten-2-one | 110-93-0 | 柑橘味 Citrusy | 果香型 Fruity |
(±)-茶螺烷 Theaspirane | 36431-72-8 | 辛辣木质香 Spicy woody | 草药香型 Herbal |
对甲酚 4-Methylphenol | 106-44-5 | 酚类芳香味 Phenolic | 化学型 Chemical |
对甲基苯甲醚 4-Methylanisole | 104-93-8 | 坚果味 Nutty | 化学型 Chemical |
对苯二甲醚 1,4-Dimethoxybenzene | 150-78-7 | 甜味,茴香味Sweet,fennel | 清香型 Green |
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