Antifungal Mechanism of Isooctyl Alcohol on Botrytis cinerea Causing Tomato Gray Mold

doi:10.16420/j.issn.0513-353x.2024-0329

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (3): 761-772.doi: 10.16420/j.issn.0513-353x.2024-0329

• Plant Protection • Previous Articles Next Articles

Antifungal Mechanism of Isooctyl Alcohol on Botrytis cinerea Causing Tomato Gray Mold

WANG Chunwei, WANG Yan, DUAN Tiankun, SU Yaxin, YUAN Shengnan, REN Lu, ZHAO Xiaojun, WANG Meiqin^*()

College of Plant Protection，Shanxi Agricultural University，Taigu，Shanxi 030801，China

Received:2024-11-06 Revised:2024-12-09 Online:2025-03-25 Published:2025-03-25
Contact: WANG Meiqin

Abstract

Abstract:

Previous findings showed that isooctyl alcohol could effectively inhibit Botrytis cinerea mycelial growth. To clarify the antifungal mechanism of isooctyl alcohol on Botrytis cinerea，the inhibition effects of isooctyl alcohol on mycelia growth，spore germination and sclerotium growth were detected by using two-sealed-base-plate method. After treatment of isooctyl alcohol，the DNA content and reactive oxygen species in B. cinerea were detected by using a laser scanning confocal microscope. The effects of isooctyl alcohol on the mycelase activity and oxalic acid production were measured using ultraviolet spectrophotometry. The control effect of isoctyl alcohol on tomato gray mold was conducted. The effects of isoctyl alcohol on the activities of defense enzymes were further detected. The results showed that isoctyl alcohol had significant inhibition effects on the mycelial growth. After treatment with isooctyl alcohol at the concentration of 20 μL · L^-1，spore germination was completely inhibited with the inhibition rate of 100%，and sclerotium was also significantly inhibited. Fluorescence intensity of DNA content in B. cinerea was dramatically reduced with the increase of reactive oxygen species level. The activities of superoxide dismutase（SOD），catalase（CAT），polygalacturonase（PG），β-1,3-glucanase（β-1,3-GA）and chitinase（CHI）were all decreased in B. cinerea. Oxalic acid（OA），an important virulence factor，was inhibited significantly in B. cinerea. In addition，the control effect of isooctyl alcohol on tomato gray mold was 77.19%. Isooctyl alcohol also enhanced SOD，CAT，CHI，peroxidase（POD）and phenylalanine aminolyase（PAL）activities in tomato. Therefore，isooctyl alcohol could effectively inhibit B. cinerea growth by interfering in the normal physiological activity，and also induce resistance to B. cinerea in tomato fruit.

Key words: tomato, isooctyl alcohol, Botrytis cinerea, antifungal effect, defense enzyme

WANG Chunwei, WANG Yan, DUAN Tiankun, SU Yaxin, YUAN Shengnan, REN Lu, ZHAO Xiaojun, WANG Meiqin. Antifungal Mechanism of Isooctyl Alcohol on Botrytis cinerea Causing Tomato Gray Mold[J]. Acta Horticulturae Sinica, 2025, 52(3): 761-772.

Figures/Tables 8

Fig. 1 Effects of isooctyl alcohol different concentrations（20，50，100，200 and 500 μL · L-1）on mycelial growth of Botrytis cinerea of tomato *** represented significant differences between treatment and control at 0.001 levels as calculated by Student’s t-test

Fig. 2 Effect of isooctyl alcohol（20 μL · L-1）on spore germination of Botrytis cinerea of tomato

Fig. 3 Effect of isooctyl alcohol（20 μL · L-1）on sclerotium weight and sclerotium number of Botrytis cinerea of tomato Values are mean ± standard error（n = 3）. Statistical analysis was conducted by Student’s t-test method. *** P < 0.001

Fig. 4 Effect of isooctyl alcohol（20 μL · L-1）on DNA content and ROS of Botrytis cinerea

Fig. 5 Effect of isooctyl alcohol（20 μL · L-1）on enzyme activities of Botrytis cinerea * and *** represented significant differences at 0.05 and 0.001 levels as calculated by Student’s t-test

Fig. 6 Effect of isooctyl alcohol（20 μL · L-1）on oxalic acid content of Botrytis cinerea Values are mean ± standard error（n = 3）. Statistical analysis was conducted by Student’s t-test method. ** P < 0.01，***P < 0.001

Fig. 7 Effects of isooctyl alcohol（20 μL · L-1）on incidence and lesion diameter of tomato Values are mean ± standard error（n = 3）. Statistical analysis was conducted by Student’s t-test method. ***P < 0.001

Fig. 8 Effects of isooctyl alcohol on the activities of tomato defense enzyme *，** and *** represented significant differences at 0.05，0.01 and 0.001 levels as calculated by Student’s t-test

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Antifungal Mechanism of Isooctyl Alcohol on Botrytis cinerea Causing Tomato Gray Mold

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