Study on the Regulation of Lactate Dehydrogenase on Mycelia Growth of Pleurotus ostreatus Under Low Temperature and Light

doi:10.16420/j.issn.0513-353x.2023-0955

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (12): 2886-2894.doi: 10.16420/j.issn.0513-353x.2023-0955

• Cultivation·Physiology & Biochemistry • Previous Articles Next Articles

Study on the Regulation of Lactate Dehydrogenase on Mycelia Growth of Pleurotus ostreatus Under Low Temperature and Light

WU Baizeng¹^,², JIANG Yuqing¹, HE Qi¹^,³, HUANG Chenyang¹, ZHANG Lijiao¹, ZHAO Mengran¹()

¹National Key Laboratory for Efficient Utilization of Arid and Semi-Arid Cultivated Land in North China，Key Laboratory of Microbial Resources Collection and Preservation，Ministry of Agriculture and Rural Affairs，Institute of Agricultural Resources and Regional Planning，Chinese Academy of Agricultural Sciences，Beijing 100081，China
²College of Horticulture，Jilin Agriculture University，Changchun 130118，China
³Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi，Jilin Agricultural University，Changchun 130118，China

Received:2024-09-10 Revised:2024-11-04 Online:2024-12-25 Published:2024-12-13
Contact: ZHAO Mengran

Abstract

Abstract:

In order to study the effect of lactate dehydrogenase on mycelial growth of Pleurotus ostreatus under the environmental stimulation of low temperature and light，the expression profiles of lactate dehydrogenase were analyzed，and the influences of exogenous lactate dehydrogenase inhibitor，lactic acid，and pyruvic acid on the mycelial growth were observed. The results showed that lactate dehydrogenase could accelerate mycelial growth in 12 h of light alternated with 12 h of darkness at 15 ℃. Inhibition of lactate dehydrogenase enzyme activity would cause the activity of pyruvate dehydrogenase （PDH）to be decreased by 15.02%，which giving rise to the decreasing flux of pyruvic acid into the tricarboxylic acid cycle，the production of ATP reduced by 14.78%，and the intracellular contents of lactic acid and pyruvic acid increased by 18.97% and 12.57%，respectively. The accumulation of lactic acid and pyruvic acid could induce increased activities of alcohol dehydrogenase，which leading to the increase of NAD⁺ content and the imbalance of NADH/ NAD⁺ ratio，and therefore the inhibition of mycelial growth.

Key words: Pleurotus ostreatus, lactate dehydrogenase, lactate, glycometabolism, low temperature, light

WU Baizeng, JIANG Yuqing, HE Qi, HUANG Chenyang, ZHANG Lijiao, ZHAO Mengran. Study on the Regulation of Lactate Dehydrogenase on Mycelia Growth of Pleurotus ostreatus Under Low Temperature and Light[J]. Acta Horticulturae Sinica, 2024, 51(12): 2886-2894.

Figures/Tables 6

Table 1 Primers for qPCR

引物 Primer	正向序列（5′-3′） Forward sequence	反向序列（5′-3′） Reverse sequence
qPCR-LDH1	GGTCCTGCTTTGTCTCGTAG	GGGAAAGGATGGAGTACAGATG
qPCR-LDH2	AGAGGAATTGGAAAGACATGGG	ATACGGAATGACGGGCATAAG
qPCR-LDH3	CTTGGGAGATGTGATACTGGG	GTTCTCGGTTTGAATTCGCTG
qPCR-LDH4	GCGATTAGTGGCAAGGTTTAC	CATCCAACATGAAATCGGCG
qPCR-LDH5	CACCCGCTCTACCAATTCTG	GCCATTCCTTTACGCTCAATC
qPCR-LDH6	TGCTTCTACCCGTTTCTGTC	ATATGACCCAATACATCCACCG
qPCR-LDH7	TGAACTTGCGTCTACTTGGTG	CAGGCGGAGAGGTTGATATG
qPCR-LDH8	GGCGGAGGTGTCAAGATTC	ATGGCGGAACGAAGGATATG

Fig. 1 Colonial phenotype（A）and intracellular ATP content（B）of Pleurotus ostreatus under the stimulation of low temperature and light Dark：24 h in dark；Light：12 h in light /12 h in dark. Different lowercase letters indicated significant difference among treatments based on Duncan’s method（P < 0.05）. The same below.

Fig. 2 Gene expression and enzyme activity of LDH in mycelia of Pleurotus ostreatus under stimulation of low temperature and light

Fig. 3 Effects of exogenous LDH inhibitors on LDH activity，mycelial growth rate and colony morphology of Pleurotus ostreatus at 28 ℃ in dark for six days

Fig. 4 Effects of exogenous LDH inhibitors on LA，PA and other related indexes in mycelia of Pleurotus ostreatus at 28 ℃ in dark for six days Student’s t test，* for P < 0.05，** for P < 0.01，*** for P < 0.001，ns for no significant difference.

Fig. 5 The effects of exogenous PA and LA on mycelial growth rate and ADH enzyme activity of Pleurotus ostreatus at 28 ℃ in dark for six days

References 20

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Study on the Regulation of Lactate Dehydrogenase on Mycelia Growth of Pleurotus ostreatus Under Low Temperature and Light

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