Identification and Analysis of Complete Genomic Sequence of Broad Bean Wilt Virus 2 Beijing Rose Isolate

doi:10.16420/j.issn.0513-353x.2023-0884

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (10): 2320-2328.doi: 10.16420/j.issn.0513-353x.2023-0884

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Identification and Analysis of Complete Genomic Sequence of Broad Bean Wilt Virus 2 Beijing Rose Isolate

LIANG Yuqing¹, SUN Chunhui¹, DENG Congliang², SHI Xiju², ZHONG Yan²^,^*(), LI Yongqiang¹^,^*()

¹ College of Bioscience and Resource Environment，Beijing University of Agriculture，Beijing 102206，China
² Science and Technology Research Center of China Customs，Beijing 102206，China

Received:2024-07-23 Revised:2024-09-14 Online:2024-10-25 Published:2024-10-21
Contact: ZHONG Yan, LI Yongqiang

Abstract

Abstract:

In order to detect the pathogen of rose plants with yellow and stunt in Huairou，Beijing，small RNA deep sequencing（sRNA）and reverse transcription-polymerase chain reaction（RT-PCR）were conducted with leaf samples collected from the diseased plants. Broad bean wilt virus 2（BBWV2）was identified after sRNA deep sequencing followed by bioinformatic analyses. To further characterize the identified BBWV2（BBWV2-rose），the complete genome sequence was cloned with primer pairs designed according to the assembled contigs. Sequence analysis showed BBWV2-rose RNA1 and RNA2 were 5 903 and 3 535 nt，respectively. Phylogenetic tree analysis based on RNA1 and RNA2 CP gene sequences suggests that BBWV2-Rose RNA1 and isolate BBWV2-CN：SY：Chenopodium album（MN786954）clustered together，and BBWV2-Rose RNA2 and isolate BBWV2-CN：SY Chenopodium album（MN786955）clustered together，which was closely related. According to Fabavirus classification criteria，the virus should be an isolate of BBWV2.

Key words: rose, deep sequencing, broad bean wilt virus 2

LIANG Yuqing, SUN Chunhui, DENG Congliang, SHI Xiju, ZHONG Yan, LI Yongqiang. Identification and Analysis of Complete Genomic Sequence of Broad Bean Wilt Virus 2 Beijing Rose Isolate[J]. Acta Horticulturae Sinica, 2024, 51(10): 2320-2328.

Figures/Tables 5

References 40

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病毒序列 Virus sequence	引物名称 Primer name	引物序列（5′-3′） Sequence	片段大小/bp Length
BBWV2	BBWV2-F	TCTCATTCAACGCCACAGGA	530
BBWV2	BBWV2-R	TGGCCAGCAAATTCCACATT	530
RNA1	BBWV2-1-F1	AAACAAACAGCTTTCGTTCCG	1 080
	BBWV2-1-R1	CTGAAATAGCTGCCATAGCAG	1 080
	BBWV2-1-F2	TTTGCAGCGTGGATGCC	1 075
	BBWV2-1-R2	CTTGTAGCCTGACCAAAAAGG	1 075
	BBWV2-1-F3	SGCAGATTGGTTATSWGAR	880
	BBWV2-1-R3	ACCGTGAGRCCCACWAR	880
	BBWV2-1-F4	MGRCGAAGRCACGCAGTRRT	1 083
	BBWV2-1-R4	AAGCACACTTGTGTYCTMACAT	1 083
	BBWV2-1-F5	CAAAGTGATRTKCCTAAYC	1 561
	BBWV2-1-R5	CCRTCAAAYCTGCTGTAA	1 561
	BBWV2-1-F6	CGSCTWGATCGTGGTCC	1 766
	BBWV2-1-R6	TTCCCTCACTACTGAAATTTACTC	1 766
RNA2	BBWV2-2-F1	CAAACAGCTTTCGTTCCGAA	927
	BBWV2-2-R1	AAGCCATCTGCCACCAATTC	927
	BBWV2-2-F2	CGCATA(G)GTC(T)TCTT(C)GTA(G)GCA(T)GA	900
	BBWV2-2-R2	GCCATGTCAGGTGCTGGA	900
	BBWV2-2-F3	TACAAT(C)TTG(A)CCTGGG(A)CCTAGC	1 119
	BBWV2-2-R3	CATGTTCCCAACCTTTTCAATGT	1 119
	BBWV2-2-F4	ATTT(C)TA(G)GATTTTACT(C)CTC(T)ATGAG	1 163
	BBWV2-2-R4	AGGTCATGGAACCCATTTTAATGG	1 163

病毒序列 Virus sequence	引物名称 Primer name	引物序列（5′-3′） Sequence	片段大小/bp Length
BBWV2	BBWV2-F	TCTCATTCAACGCCACAGGA	530
BBWV2	BBWV2-R	TGGCCAGCAAATTCCACATT	530
RNA1	BBWV2-1-F1	AAACAAACAGCTTTCGTTCCG	1 080
	BBWV2-1-R1	CTGAAATAGCTGCCATAGCAG	1 080
	BBWV2-1-F2	TTTGCAGCGTGGATGCC	1 075
	BBWV2-1-R2	CTTGTAGCCTGACCAAAAAGG	1 075
	BBWV2-1-F3	SGCAGATTGGTTATSWGAR	880
	BBWV2-1-R3	ACCGTGAGRCCCACWAR	880
	BBWV2-1-F4	MGRCGAAGRCACGCAGTRRT	1 083
	BBWV2-1-R4	AAGCACACTTGTGTYCTMACAT	1 083
	BBWV2-1-F5	CAAAGTGATRTKCCTAAYC	1 561
	BBWV2-1-R5	CCRTCAAAYCTGCTGTAA	1 561
	BBWV2-1-F6	CGSCTWGATCGTGGTCC	1 766
	BBWV2-1-R6	TTCCCTCACTACTGAAATTTACTC	1 766
RNA2	BBWV2-2-F1	CAAACAGCTTTCGTTCCGAA	927
	BBWV2-2-R1	AAGCCATCTGCCACCAATTC	927
	BBWV2-2-F2	CGCATA(G)GTC(T)TCTT(C)GTA(G)GCA(T)GA	900
	BBWV2-2-R2	GCCATGTCAGGTGCTGGA	900
	BBWV2-2-F3	TACAAT(C)TTG(A)CCTGGG(A)CCTAGC	1 119
	BBWV2-2-R3	CATGTTCCCAACCTTTTCAATGT	1 119
	BBWV2-2-F4	ATTT(C)TA(G)GATTTTACT(C)CTC(T)ATGAG	1 163
	BBWV2-2-R4	AGGTCATGGAACCCATTTTAATGG	1 163

Identification and Analysis of Complete Genomic Sequence of Broad Bean Wilt Virus 2 Beijing Rose Isolate

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