The Creation and Study on Characteristics of New Material of Autotetraploid Purple Tsai-tai

doi:10.16420/j.issn.0513-353x.2022-0686

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (7): 1419-1428.doi: 10.16420/j.issn.0513-353x.2022-0686

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

The Creation and Study on Characteristics of New Material of Autotetraploid Purple Tsai-tai

ZHANG Chen¹, LI Mengjie¹, YANG Xiaoxue¹, WANG Meiyun¹, XIAO Dong¹, WANG Jianjun¹, HOU Xilin¹^,², HU Jun³, LIU Tongkun¹^,^*()

¹ State Key Laboratory of Crop Genetics and germplasm innovation，College of Horticulture，Nanjing Agricultural University，Nanjing 210095，China
² Nanjing Suman Plasma Engineering Research Institute of Agricultural Sciences，Nanjing 210095，China
³ Agricultural and Rural Bureau of Luoshe Town，Huishan District，Wuxi，Jiangsu 214187，China

Received:2023-01-06 Revised:2023-04-11 Online:2023-07-25 Published:2023-07-26
Contact: LIU Tongkun

Abstract

Abstract:

In order to create new germplasm resource of tetraploid purple tsai-tai（Brassica campestris var. purpurea Bailey），0.2 g · L^-1 colchicine solution was used to drip the apical shoot of diploid. Then，autotetraploid plants were identified by morphology，anatomy and cytology methods. Through the analysis of agronomic characters，the plant height，spreading degree，leaf width and leaf stalk length of tetraploid plants increased significantly. However，the number of leaves of tetraploid significantly reduced. In nutritional quality，the content of soluble protein and anthocyanin in tetraploid plants increased significantly，while the content of soluble sugar，fibrin and nitric nitrogen in tetraploid plants significantly reduced compared with diploid. The analysis of photosynthetic characteristics showed that the net photosynthetic rate，interintracellular CO₂ concentration，stomatal conductance and transpiration rate of tetraploid plant were higher than that ofdiploid，indicating that tetraploid plants were more adaptable to strong light. The analysis of disease resistance showed that the tetraploid plants had stronger disease resistance.

Key words: Brassica campestris var. purpurea, autotetraploid, colchicine, photosynthetic characteristics, Botrytis cinerea

ZHANG Chen, LI Mengjie, YANG Xiaoxue, WANG Meiyun, XIAO Dong, WANG Jianjun, HOU Xilin, HU Jun, LIU Tongkun. The Creation and Study on Characteristics of New Material of Autotetraploid Purple Tsai-tai[J]. Acta Horticulturae Sinica, 2023, 50(7): 1419-1428.

Figures/Tables 9

References 28

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引物名称 Primer name	引物序列（5′-3′） Primer sequence
Actin-Q	F：AGGCTACACGTTCGGACAAG
	R：TGGGGCACTAAACACAGTCA
Botrytis cinerea Actin-Q	F：AATGTTACCATACAAATCCTTACGGACA
	R：ACTCATATGTTGGAGATGAAGCGCAA

引物名称 Primer name	引物序列（5′-3′） Primer sequence
Actin-Q	F：AGGCTACACGTTCGGACAAG
	R：TGGGGCACTAAACACAGTCA
Botrytis cinerea Actin-Q	F：AATGTTACCATACAAATCCTTACGGACA
	R：ACTCATATGTTGGAGATGAAGCGCAA

材料 Material	株高/cm Plant height	叶片数 Number of leaves	单株质量/g Weight per plant	叶片长/cm Leaf length	叶片宽/cm Leaf width
2x	30.28 ± 0.50	18.20 ± 0.66	231.37 ± 30.79	16.58 ± 0.27	14.44 ± 0.17
4x	33.02 ± 0.54^**	14.40 ± 0.92^**	272.16 ± 29.98	17.02 ± 0.42	15.40 ± 0.31^*
材料 Material	叶柄长/cm Petiole length	叶柄宽/cm Petiole width	叶柄厚/mm Thickness of petiole	十叶厚/mm Ten leaves thickness	开展度/（cm×cm） Divergence of plant
2x	14.48 ± 0.74	1.10 ± 0.12	0.92 ± 0.04	4.10	（37.46 ± 3.08）×（38.12 ± 0.84）
4x	18.56 ± 0.20^*	1.36 ± 0.08	1.12 ± 0.08	4.83	（45.62 ± 1.33）×（38.24 ± 1.65）^*

材料 Material	株高/cm Plant height	叶片数 Number of leaves	单株质量/g Weight per plant	叶片长/cm Leaf length	叶片宽/cm Leaf width
2x	30.28 ± 0.50	18.20 ± 0.66	231.37 ± 30.79	16.58 ± 0.27	14.44 ± 0.17
4x	33.02 ± 0.54^**	14.40 ± 0.92^**	272.16 ± 29.98	17.02 ± 0.42	15.40 ± 0.31^*
材料 Material	叶柄长/cm Petiole length	叶柄宽/cm Petiole width	叶柄厚/mm Thickness of petiole	十叶厚/mm Ten leaves thickness	开展度/（cm×cm） Divergence of plant
2x	14.48 ± 0.74	1.10 ± 0.12	0.92 ± 0.04	4.10	（37.46 ± 3.08）×（38.12 ± 0.84）
4x	18.56 ± 0.20^*	1.36 ± 0.08	1.12 ± 0.08	4.83	（45.62 ± 1.33）×（38.24 ± 1.65）^*

营养指标 Nutritional index	材料Material
营养指标 Nutritional index	2x	4x
可溶性糖含量/（mg · g^-1）Soluble sugar content	23.46 ± 0.17	20.89 ± 0.09^**
可溶性蛋白含量/（mg · g^-1）Soluble protein content	26.90 ± 0.28	28.86 ± 0.36^*
纤维素含量/（μg · g^-1）Fibrin content	194.87 ± 1.19	111.28 ± 1.01^**
花青苷含量/（mg · g^-1）Anthocyanin content	0.13 ± 0.00	0.17 ± 0.01^*
叶绿素含量/（mg · g^-1）Chlorophyll content	9.74 ± 1.65	9.42 ± 0.80
硝态氮含量/（μg · g^-1）Nitric nitrogen content	13.79 ± 0.53	12.11 ± 0.25^*

The Creation and Study on Characteristics of New Material of Autotetraploid Purple Tsai-tai

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材料 Material	最大净光合速率/ （μmol · m^-2 · s^-1） P_n，max	表观量子效率 AQY	光饱和点/ （μmol · m^-2 · s^-1） LSP	光补偿点/ （μmol · m^-2 · s^-1） LCP	暗呼吸速率/ （μmol · m^-2 · s^-1） R_day
2x	32.06 ± 0.39	0.13 ± 0.14	257.51 ± 14.13	6.76 ± 0.76	0.68 ± 0.35
4x	47.54 ± 4.25^*	0.11 ± 0.03^*	487.48 ± 0.00^*	9.87 ± 1.71^*	1.12 ± 0.12

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