Recent Advances in Molecular Biology of Crassulacean Acid Metabolism Plants and the Application Potential of CAM Engineering

doi:10.16420/j.issn.0513-353x.2021-0835

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (12): 2597-2610.doi: 10.16420/j.issn.0513-353x.2021-0835

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Recent Advances in Molecular Biology of Crassulacean Acid Metabolism Plants and the Application Potential of CAM Engineering

LI Junzhang¹, QIN Yuan²^,³^,⁴, XIAO Qiang¹, AN Chang²^,⁴, LIAO Jingyi³, ZHENG Ping²^,^*()

¹College of Agriculture，Fujian Agriculture and Forestry University，Fuzhou 350000，China
²Haixia Institute of Science and Technology，Fujian Agriculture and Forestry University，Fuzhou 350000，China
³College of Life Science，Fujian Agriculture and Forestry University，Fuzhou 350000，China
⁴College of Agriculture，Guangxi University，Nanning 530000，China

Received:2022-04-22 Revised:2022-08-31 Online:2022-12-25 Published:2023-01-02
Contact: ZHENG Ping E-mail:zhengping@fafu.edu.cn

Abstract

Abstract:

Crassulacean acid metabolism photosynthetic pathway is a kind of carbon concentrating mechanism that evolved from C3 photosynthesis and it is characterized by nocturnal CO₂ fixation and high water use efficiency. Currently，there are growing interests in improving WUE to enhance drought resistance by“transferring”CAM pathway into C₃ crops，also called‘CAM engineering’. CAM engineering has significant application potential in agriculture，while systematical investigations of CAM photosynthetic pathway / plants are a prerequisite to its progression. The wide applications of multi-omics and molecular biology methods have extensively promoted CAM plants related studies. CAM pathway-related key genes and their functions are being gradually revealed，and many CAM plant genomes have been published. Diverse mechanisms are involved in the regulation of the CAM pathway, including transcription factors，hormones，miRNA，lncRNA，alternative splicing，and DNA methylation This article mainly focuses on the latest progress of molecular biology and omics studies on CAM plants. In addtion，future research prospective and study interests are also discussed to guide further investigations on CAM plants.

Key words: crassulacean acid, metabolism, multi-omics, molecular mechanism, transcriptional regulation, water use efficiency

CLC Number:

S6
Q 945.11

LI Junzhang, QIN Yuan, XIAO Qiang, AN Chang, LIAO Jingyi, ZHENG Ping. Recent Advances in Molecular Biology of Crassulacean Acid Metabolism Plants and the Application Potential of CAM Engineering[J]. Acta Horticulturae Sinica, 2022, 49(12): 2597-2610.

Figures/Tables 2

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植物 Plant	CAM类型 CAM type	组装水平 Assembly level	基因组/Mb Genome size	N50/kb	文献 Reference
玉吊钟Kalanchoe fedtschenkoi	组成型 Constitutive	支架水平 Scaffold	256	2 450（scaffold）	Yang et al.，2017
菠萝Ananas comosus	组成型Constitutive	染色体 Chromosome	382	11 800（scaffold）	Ming et al.，2015
红菠萝Ananas comosus var. bracteatus CB5	组成型Constitutive	染色体 Chromosome	513	427（contig）	Chen et al.，2019
玉米石Sedum album	组成型Constitutive	重叠群 Contig	302	93（contig）	Wai et al.，2019
麻疯树Jatropha curcas	兼性Facultative	支架水平 Scaffold	339	15 400（scaffold）	Ha et al.，2019
台湾蝴蝶兰Phalaenopsis aphrodite	组成型Constitutive	染色体 Chromosome	1 200	19 700（scaffold）	Chao et al.，2018
深圳拟兰Apostasia shenzhenica	组成型Constitutive	染色体 Chromosome	349	3 029（scaffold）	Zhang et al.，2017
小兰屿蝴蝶兰Phalaenopsis equestris	组成型Constitutive	支架水平 Scaffold	~980	359.1（scaffold）	Cai et al.，2015
黄石斛Dendrobium catenatum	兼性Facultative	支架水平 Scaffold	1 010	391（scaffold）	Zhang et al.，2016
铁皮石斛Dendrobium officinale	兼性Facultative	染色体 Chromosome	1 230	63 070（scaffold）	Niu et al.，2021

植物 Plant	CAM类型 CAM type	组装水平 Assembly level	基因组/Mb Genome size	N50/kb	文献 Reference
玉吊钟Kalanchoe fedtschenkoi	组成型 Constitutive	支架水平 Scaffold	256	2 450（scaffold）	Yang et al.，2017
菠萝Ananas comosus	组成型Constitutive	染色体 Chromosome	382	11 800（scaffold）	Ming et al.，2015
红菠萝Ananas comosus var. bracteatus CB5	组成型Constitutive	染色体 Chromosome	513	427（contig）	Chen et al.，2019
玉米石Sedum album	组成型Constitutive	重叠群 Contig	302	93（contig）	Wai et al.，2019
麻疯树Jatropha curcas	兼性Facultative	支架水平 Scaffold	339	15 400（scaffold）	Ha et al.，2019
台湾蝴蝶兰Phalaenopsis aphrodite	组成型Constitutive	染色体 Chromosome	1 200	19 700（scaffold）	Chao et al.，2018
深圳拟兰Apostasia shenzhenica	组成型Constitutive	染色体 Chromosome	349	3 029（scaffold）	Zhang et al.，2017
小兰屿蝴蝶兰Phalaenopsis equestris	组成型Constitutive	支架水平 Scaffold	~980	359.1（scaffold）	Cai et al.，2015
黄石斛Dendrobium catenatum	兼性Facultative	支架水平 Scaffold	1 010	391（scaffold）	Zhang et al.，2016
铁皮石斛Dendrobium officinale	兼性Facultative	染色体 Chromosome	1 230	63 070（scaffold）	Niu et al.，2021

植物（文献） Plant（Reference）	CAM类型 CAM type	转录因子 Transcription factor	转录因子家族 Transcription factor family	拟南芥同源基因 Arabidopsis homolog
冰叶日中花 Mesembryanthemum crystallinum （Amin et al.，2019）	兼性 Facultative	McERF74	AP2/ERF/CRF	AT1G53910
		McNAC29	NAC	AT1G69490
		McBLH1	HB/Homeodomain	AT2G35940
		McbZIP2	bZIP	AT2G18160
		McAGL8	MADS/AGAMOUS-LIKE8	AT5G60910
		McAP2-12	AP2/ERF	AT1G53910
		McbZIP44	bZIP	AT1G75390
		McHB7	HB/Homeodomain	AT2G46680
玉吊钟 Kalanchoe fedtschenkoi （Yang et al.，2017）	组成型 Constitutive	KfMYB59^*	MYB	AT5G59780
		KfLHY1	Homeodomain	AT1G01060
		KfbZIP29	bZIP	AT4G38900
		KfNF-YB3	NF-Ys	AT4G14540
		KfNAC83^*	NAC	AT4G13180
		KfAP2	AP2/ERF/CRF	AT4G11140
		KfCOL3	Zinc Finger CONSTANS-like 4	AT5G24930
		KfCOL5	Zinc Finger CONSTANS-like 5	AT5G67660
棱轴土人参 Talinum triangulare （Maleckova et al.，2019）	兼性 Facultative	TtOFP8	-	AT5G19650
		TtNF-YA9	NF-YA	AT3G20910
		TtNF-YB3	NF-YB	AT4G14540
		TtLBD21	LBD	AT3G11090
		TtHB-1	HD-ZIP	AT3G01470
		TtHSFA2	HSF	AT2G26150
		TtBBX15	CO-like	AT1G25440
		TtMP	ARF	AT1G19850
		-	Trihelix	AT2G44730
		-	-	AT4G00990
		TtHSFC1	HSF	AT3G24520

植物（文献） Plant（Reference）	CAM类型 CAM type	转录因子 Transcription factor	转录因子家族 Transcription factor family	拟南芥同源基因 Arabidopsis homolog
冰叶日中花 Mesembryanthemum crystallinum （Amin et al.，2019）	兼性 Facultative	McERF74	AP2/ERF/CRF	AT1G53910
		McNAC29	NAC	AT1G69490
		McBLH1	HB/Homeodomain	AT2G35940
		McbZIP2	bZIP	AT2G18160
		McAGL8	MADS/AGAMOUS-LIKE8	AT5G60910
		McAP2-12	AP2/ERF	AT1G53910
		McbZIP44	bZIP	AT1G75390
		McHB7	HB/Homeodomain	AT2G46680
玉吊钟 Kalanchoe fedtschenkoi （Yang et al.，2017）	组成型 Constitutive	KfMYB59^*	MYB	AT5G59780
		KfLHY1	Homeodomain	AT1G01060
		KfbZIP29	bZIP	AT4G38900
		KfNF-YB3	NF-Ys	AT4G14540
		KfNAC83^*	NAC	AT4G13180
		KfAP2	AP2/ERF/CRF	AT4G11140
		KfCOL3	Zinc Finger CONSTANS-like 4	AT5G24930
		KfCOL5	Zinc Finger CONSTANS-like 5	AT5G67660
棱轴土人参 Talinum triangulare （Maleckova et al.，2019）	兼性 Facultative	TtOFP8	-	AT5G19650
		TtNF-YA9	NF-YA	AT3G20910
		TtNF-YB3	NF-YB	AT4G14540
		TtLBD21	LBD	AT3G11090
		TtHB-1	HD-ZIP	AT3G01470
		TtHSFA2	HSF	AT2G26150
		TtBBX15	CO-like	AT1G25440
		TtMP	ARF	AT1G19850
		-	Trihelix	AT2G44730
		-	-	AT4G00990
		TtHSFC1	HSF	AT3G24520

Recent Advances in Molecular Biology of Crassulacean Acid Metabolism Plants and the Application Potential of CAM Engineering

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