https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (3): 577-589.doi: 10.16420/j.issn.0513-353x.2020-0334

• Research Notes • Previous Articles     Next Articles

Cloning and Analysis of MoDXS Gene and Its Promoter in Morinda officinalis

XIE Dejin1, ZHOU Chengcheng2, YANG Ke1, REN Ke1, YANG Deming1, CHEN Lingyan2, RONG Jundong1, ZHENG Yushan1,2,*()   

  1. 1College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China
    2College of Landscape,Fujian Agriculture and Forestry University,Fuzhou 350002,China
  • Received:2020-09-29 Online:2021-03-25 Published:2021-04-02
  • Contact: ZHENG Yushan E-mail:zys1960@163.com

Abstract:

In the root tissue of Morinda officinalis,three full-length cDNA sequences of 1-deoxy-D-xylulose 5-phosphate synthase genes(MoDXS1,MoDXS2-1 and MoDXS2-2)were successfully cloned and their length were 2 676,2 667,and 2 610 bp,respectively. The coding sequence(CDS)of the three cDNAs were 2 154,2 121,and 2 190 bp,encoding 717,706,and 729 amino acid residues,respectively. Sequence comparison by BlastP in NCBI database revealed that MoDXS proteins had high homology with DXS proteins from other species. The result of the phylogenetic tree displayed that MoDXS proteins had the closest relationship with DXS proteins from Coffea canephora and Coffea arabica. Furthermore,MoDXS1,MoDXS2-1,and MoDXS2-2 proteins were grouped into clade 1,clade 3, and clade 2,respectively. MoDXS1 gene had the highest expression level in roots. The expression level of MoDXS2-1 gene had significant differences in three tissues,and the expression level was the highest in leaves. However,the expression level of MoDXS2-2 gene was the highest in roots but lower in stems and leaves. The plantCARE analysis indicated that the upstream regulation sequences of MoDXS1,MoDXS2-1,and MoDXS2-2 were 2 538,732,and 1 744 bp,respectively. The subcellular localization exhibited that the three MoDXS proteins were located on chloroplast.

Key words: Morinda officinalis, 1-deoxy-D-xylulose 5-phosphate synthase, promoter sequence, subcellular localization

CLC Number: