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园艺学报 ›› 2018, Vol. 45 ›› Issue (6): 1031-1040.doi: 10.16420/j.issn.0513-353x.2017-0571

• 研究论文 • 上一篇    下一篇

过表达苹果多肽激素基因MdCEP1促进花青苷积累

李 睿,安建平,由春香,王小非*,郝玉金*   

  1. 山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,农业部黄淮地区园艺作物生物学与种质创制重点实验室,山东泰安 271018
  • 出版日期:2018-06-25 发布日期:2018-06-25
  • 基金资助:
    国家自然科学基金项目(31430074);教育部创新团队支持计划项目(IRT15R42);山东省现代农业产业技术体系项目(SDAIT-06-03)

Overexpression of Apple Peptide Hormone Gene MdCEP1 Promotes the Accumulation of Anthocyanins

LI Rui,AN Jianping,YOU Chunxiang,WANG Xiaofei*,and HAO Yujin*   

  1. State Key Laboratory of Crop Biology,MOA Key Laboratory of Horticultural Crop Biology(Huanghuai Region)and Germplasm Innovation,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China
  • Online:2018-06-25 Published:2018-06-25

摘要: 以‘王林’苹果(Malus × domestica‘Orin’)愈伤组织为试材,初步探讨苹果多肽激素MdCEP1(C-TERMINALLY ENCODED PEPTIDE1)在调控花青苷合成方面的作用。分析显示MdCEP1位于苹果第15号染色体,只有1个外显子。蛋白序列比对显示不同物种中CEP结构域非常保守。启动子分析表明,MdCEP1启动子序列包含多个顺式作用元件,包括与分生组织有关的CAT-box元件、赤霉素响应元件(P-box)、光响应元件(MNF1)和与类黄酮合成相关的MYB类蛋白结合位点(MBSI)。通过农杆菌介导的遗传转化获得MdCEP1转基因苹果愈伤组织,进一步分析发现过表达MdCEP1能够明显促进愈伤组织花青苷积累,并且促进花青苷合成相关基因的表达。MdCEP1在拟南芥中异位表达,同样能够促进拟南芥中花青苷的积累,并且促进拟南芥花青苷合成相关基因的表达。研究结果表明,MdCEP1能够正调控苹果花青苷的合成。

关键词: 苹果, 多肽激素, MdCEP1, 花青苷

Abstract: The effects of apple peptide hormone MdCEP1(C-TERMINALLY ENCODED PEPTIDE1)on the regulation of anthocyanin synthesis were investigated by using Malus × domestica‘Orin’as the test material,to provide reference for further study on the mechanism of anthocyanin synthesis and characterization of MdCEP1 function. Chromosomal localization analysis revealed that MdCEP1 was located on the chromosome 15 of the apple genome and had only one exon. Protein sequence alignment showed that CEP domains were conserved in different species. Promoter analysis showed that the MdCEP1 promoter sequence contained a number of cis-acting elements,including CAT-box elements associated with meristem,gibberellin response elements(P-boxes),light-responsive elements(MNF1)and the MYB binding site(MBSI)involved in flavonoid biosynthesis. MdCEP1 transgenic apple calli were obtained by Agrobacterium tumefaciens-mediated genetic transformation. Further analysis found that overexpression of MdCEP1 could significantly promote the anthocyanin accumulation,and promote the expression of apple anthocyanin biosynthetic genes. Ectopic expression of MdCEP1 in Arabidopsis thaliana,could also promote the accumulation of anthocyanin,and promote the expression of anthocyanin biosynthetic genes in Arabidopsis thaliana. The results showed that MdCEP1 could positively control the synthesis of anthocyanin.

Key words: apple, peptide hormone, MdCEP1, anthocyanin

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