园艺学报 ›› 2017, Vol. 44 ›› Issue (8): 1477-1485.doi: 10.16420/j.issn.0513-353x.2017-0043

• 研究论文 • 上一篇    下一篇


王慧玲1,3,*,王 伟2,*,闫爱玲1,3,孙 磊1,4,张国军1,王晓玥1,徐海英1,**   

  1. (1北京市林业果树科学研究院,北京 100093;2中国林业科学研究院林业研究所,北京 100091;3北京市落叶果树工程技术研究中心,北京 100093;4农业部华北都市农业重点实验室,北京 100093)
  • 出版日期:2017-08-25 发布日期:2017-08-25

The Expression and Subcellular Localization of Chalcone Isomerase in Developing Grape Berries

WANG Huiling1,3,*,WANG Wei2,*,YAN Ailing1,3,SUN Lei1,4,ZHANG Guojun1,WANG Xiaoyue1,and XU Haiying1,**   

  1. (1Beijing Academy of Forestry and Pomology Sciences,Beijing 100093,China;2 Institute of Forestry,The Chinese Academy of Forestry,Beijing 100091,China;3Beijing Engineering Research Center for Deciduous Fruit Trees,Beijing 100093,China;4Key Laboratory of Urban Agriculture(North China),Ministry of Agriculture,P. R. China,Beijing 100093,China)
  • Online:2017-08-25 Published:2017-08-25



关键词: 葡萄, 查尔酮异构酶, 类黄酮, 亚细胞定位

Abstract: The gene expression,protein accumulation and subcellular localization of Chalcone isomerase(CHI,EC developing grape berry(Vitis vinifera L.‘Cabernet Sauvignon’)was performed via RT-PCR,Western-blotting and immunogold electron microscopy technique using grape CHI antibody. The results showed that the quantity of CHI gene transcript and protein in developing grape berry was developmental stage dependant. The high content of CHI transcripts and protein was observed in grape berries at early development stage(20 days after flower)and veraison,corresponding well to the accumulation of total flavonoids. Immunogold localization result showed that CHI was mainly localized in cytoplasm of the young grape berry skin cells,while some gold particles representing CHI were also found in the vacuole and plastid. In the skin cells of grape berry at veraison,CHI was mainly distributed in cytoplasm,plastids and nuclear,and the gold particles representing CHI increased significantly at this stage;CHI were observed in cytoplasm at late developmental stage,a little was also investigated in the vacuole and plastids.

Key words: grape, chalcone isomerase, flavonoid, subcellular localization