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园艺学报 ›› 2009, Vol. 36 ›› Issue (3): 437-440.

• 研究简报 • 上一篇    下一篇

云南省建水县番茄上Tospovirus属病毒的初步鉴定

刘雅婷1,3;郑元仙1;李永忠2;李正跃3*   

  1. 1云南农业大学农学与生物技术学院,昆明 650201;2云南农业大学烟草学院;昆明 650201;3云南农业大学植保学院,昆明 650201)
  • 收稿日期:2008-10-09 修回日期:2009-02-04 出版日期:2009-03-25 发布日期:2009-03-25
  • 通讯作者: 李正跃

Preliminary identification of Tospovirus on tomato in Jianshui county of Yunnan province

LIU Ya-ting1,3,ZHENG Yuan-xian1,LI Yong-zhong2,LI Zheng-yue3*   

  1. (1College of Agricultural and Biotechnology, Yunnan Agricultural University,Yunnan 650201, China;2College of Tobacco Science, Yunnan Agricultural University,Yunnan 650201, China;3College of plant protection, Yunnan Agricultural University,Yunnan 650201, China)
  • Received:2008-10-09 Revised:2009-02-04 Online:2009-03-25 Published:2009-03-25
  • Contact: LI Zheng-yue

摘要: 2005-2006年对云南省主要蔬菜种植区昆明、红河和楚雄等3个地区进行调查,获得9份疑似番茄斑萎病毒属病毒的样本。通过Tospovirus组引物(Tospovirus Group Primer)进行RT-PCR扩增,其中1份来自红河州建水县番茄上的样品获得约500 bp核酸片段,通过NCBI BLAST网络数据库比对,确定为Tospovirus属的病毒;应用DNAMAN软件与已经公布的Tospovirus属病毒RNA L序列比对分析,从得到的相似性树状图可以看出,云南获得的该样品与血清组Ⅳ的Tospovirus属病毒聚为一簇。

关键词: 番茄斑萎病毒属, 番茄, 血清组

Abstract:

Nine samples possibly from genus Tospovirus were collected from major vegetable-growing field in Yunnan province, including Honghe, Chuxiong and Kunming, during 2005 - 2006 period. Around 500 bp fragments were obtained after RT-PCR were executed on 9 samples using Tospovirus Group Primers. The sequence results of around 500 bp fragments were compared in the NCBI BLAST website, one sample from tomato in Honghe county of Yuannan province was identified as Tospovirus genus. A phylogenetic tree was constructed using DNAMAN software according to the sequence of the sample and their corresponding public RNA L fragment sequences of Tospovirus. The results indicated that this sample obtained in this study fell into the Serogroup Ⅳ.

Key words: Tospovirus, Tomato, Serogroup

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