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园艺学报 ›› 2014, Vol. 41 ›› Issue (6): 1080-1088.

• 果树 • 上一篇    下一篇

葡萄白粉病菌应答基因VpSTART的克隆及表达分析

闫筱筱1,侯鸿敏2,焦 晨1,王西平1,3,*   

  1. (1西北农林科技大学园艺学院,旱区作物逆境生物学国家重点实验室,陕西杨凌 712100;2青岛农业大学园艺学院,山东青岛 266109;3农业部西北地区园艺作物生物学与种质创制重点开放实验室,陕西杨凌 712100)
  • 收稿日期:2014-01-17 出版日期:2014-06-25 发布日期:2014-06-25

Cloning and Expression Analysis of Powdery Mildew Fungus Responsive Gene VpSTART in Grape

YAN Xiao-xiao1,HOU Hong-min2,JIAO Chen1,and WANG Xi-ping1,3,*   

  1. (1College of Horticulture,State Key Laboratory of Crop Stress Biology in Arid Areas,Northwest A & F University,Yangling,Shaanxi 712100,China;2College of Horticulture,Qingdao Agricultural University,Qingdao,Shandong 266109,China;3Key Laboratory of Horticultural Crop Biology and Germplasm Development in Northwest China,Ministry of Agriculture,Yangling,Shaanxi 712100,China)
  • Received:2014-01-17 Online:2014-06-25 Published:2014-06-25

摘要: 前期获得葡萄白粉病菌应答基因VpSTART的EST基础上,采用RACE和RT-PCR技术克隆该基因cDNA全长序列。VpSTART全长1 321 bp,3′端非编码区114 bp,包含一个1 206 bp开放阅读框,编码401个氨基酸。VpSTART蛋白分子量为45.3 kD与欧亚种葡萄、玉米、拟南芥、蒺藜状苜蓿和蓖麻的蛋白同源性分别为99%、64%、58%、46%和25%。实时荧光定量PCR表明,VpSTART在‘商–24’葡萄花序、卷须和茎中表达量较高;感白粉病的‘湖南–1’葡萄叶片接种白粉病菌后VpSTART表达量与对照没有显著差异,而抗白粉病的‘商–24’葡萄接种12 h后VpSTART表达量增加,在24 ~ 96 h表现显著性差异;用SA、MeJA和Eth等不同信号分子分别处理‘湖南–1’和‘商–24’葡萄叶片1 ~ 48 h后,VpSTART基因的表达受SA负调控,受MeJA和Eth正调控。

关键词: 葡萄, 白粉病菌, VpSTART, 基因克隆, 表达分析

Abstract: Based on previous EST sequence of grape ,a full-length cDNA sequence was cloned through RACE and RT-PCR technologies in this study. The full-length cDNA of VpSTART was 1 321 bp with 114 bp 3′UTR,contained a 1 206 bp open reading frame encoding 401 amino acids residues. VpSTART protein showed 45.3 kD,and shared 99%,64%,58%,46% and 25% homology with that of Vitis viniferaZea maysArabidopsis thalianaMedicago truncatula and Ricinus communis. Real-time quantitative PCR indicated the expression level of VpSTART was high in inflorescence,tendril and stem of Shang-24 grape abundantly;Accumulation of VpSTART transcripts in Hunan-1 grape leaf that is susceptible to powdery mildew showed no significant difference with that in contrast after E. necator inoculation,while the accumulation increased in Shang-24 grape for 12 h that is resistant powdery mildew and showed a significant difference during 24–96 h inoculation;After 1–48 h treatment with SA,MeJA and Eth signal molecules in Hunan-1 and Shang-24 grapeleaf,the expression patterns of VpSTARTshowed negative regulation by SA,positive regulation by MeJA and Eth.a calculatedmolecular weightof powdery mildew fungus responsive gene VpSTART

Key words: grape, powdery mildew, VpSTART, gene clone, expression analysis

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