关键词: 葡萄, 果实, UV-C, 总黄烷–3–醇, 黄烷醇类多酚, LAR, 组织定位, 辣椒轻斑驳病毒, 辣椒, 原位RT-PCR, 地高辛, 组织定位, cDNA探针

Abstract: The 5-year old grapevine（Vitis vinifera L.‘Cabernet Sauvignon’）was subjected to the periodical UV-C irradiation during berry development. The spatial and temporal accumulation of flavanols and the enzyme activity of LAR in the berry were analyzed by spectrophotometer method，and the tissue localization of LAR1 and LAR2 were detected by the immunohistochemical localization. The results indicated that the accumulation of total phenol，flavanols，flavan-3-ols，as well as the LAR enzyme activity in the skin and flesh were induced by UV-C irradiation，which was irradiation dose-，organ/tissue-，and development stage-depend. There were no obvious changes in the tissue localization of LAR1 and LAR2 induced by UV-C，whereas an significant increasing signal was found，especially in the skin and vascular bundle. All the results suggest that UV-C irradiation could induce the accumulation of LAR1 and LAR2 enzyme protein in the skin and vascular bundle，an increasing in LAR activity in the skin during grape berry development，which resulted in the accumulation of total flavan-3-ols and flavanols.

Key words: grape, berries, UV-C, total flavan-3-ols, flavanols, LAR, tissue localization, Pepper mild mottle virus, Capsicum, in situ RT-PCR, digoxin, location in tissue, cDNA probe