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https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2001, Vol. 28 ›› Issue (3): 194-199.

• 研究论文 • 上一篇    下一篇

应用高剂量辐射花粉授粉及幼胚培养诱导苹果单倍体

何道一1;孙 山2;崔德才1;陈振光3   

  1. (1 山东农业大学生命科学学院, 泰安271018;2 山东省果树研究所, 泰安271000;3 福建农业大学园艺系, 福州350002)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-06-25 发布日期:2001-06-25

Apple Haploid Induction by Pollination with High Dose Irradiated ollen

He Daoyi1, Sun Shan2, Cui Decai1, and Chen Zhenguang3   

  1. (1 College of Biological Science Shandong Agricultural University , Tai’an 271018; 2 Shandong Institute of Pomology , Tai’an 271000; 3 Department of Horticulture Fujian Agricultural University , Fuzhou 350002)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-06-25 Published:2001-06-25

摘要: 以1 000~1 500 Gy 剂量的γ射线处理‘红星’苹果花粉, 以此花粉给‘贝拉’、‘辽伏’和‘富士’苹果授粉, 获得3 个品种的幼胚, 经幼胚培养得到各品种的单倍体植株。试验结果表明, 影响高剂量辐射花粉技术生产苹果单倍体效率的主要因素是早期落果、幼胚败育和带胚乳幼胚不能培养再生植株。初步研究表明25 日龄幼胚(胚珠) 可以培养再生植株。

关键词: 苹果, 辐射, 花粉, 单倍体, 胚培养

Abstract: The parthenogenic young embryos were gained in apple varieties (Bela , Liaofu and Fuji) due to pollination with radiated pollen by 1 000 - 1 500 Gyγ ray. The haploids of these varieties were produced by young embryo culture and regeneration. The investigation indicated that major factors which limited hapliod production efficiency in apple by such technique included abscission of
fruits in early stage , young embryo abortion and difficulty in regeneration of young embryo with endosperm. The results also suggested that regeneration of 25 d young embryo (ovule) was available.

Key words: Apple, Radiated pollen, Hapliod, Embryo culture

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