园艺学报 ›› 2005, Vol. 32 ›› Issue (02): 256-261.

• 研究论文 • 上一篇    下一篇


娄群峰1;陈劲枫1;Molly Jahn2;陈龙正1;耿红1;罗向东1   

  1. (1 作物遗传与种质创新国家重点实验室, 南京农业大学园艺学院, 南京210095; 2 康乃尔大学育种系, 伊萨卡, 纽约 14853, 美国)
  • 收稿日期:2004-06-07 修回日期:2004-07-26 出版日期:2005-04-25 发布日期:2005-04-25
  • 通讯作者: 陈劲枫

Identification of AFLP and SCAR Molecular Markers Linked to Gynoecious Loci in Cucumis sativus L.

Lou Qunfeng1;Chen Jinfeng1;Molly Jahn2;Chen Longzheng1;Geng Hong1;Luo Xiangdong1   

  1. (1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University,N anjing 210095, China; 2Department of Plant Breeding, Cornell University, Ithaca, N Y 14853, USA )
  • Received:2004-06-07 Revised:2004-07-26 Online:2005-04-25 Published:2005-04-25
  • Contact: Chen Jin-feng

摘要: 本研究以全雌品种‘戴多星’自交系和弱雌品种‘北京截头’自交系为双亲杂交获得F1 ,然后得到F2 性型分离群体, 利用分离群体分组分析法(Bulked Segregant Analysis, BSA) 构建全雌和弱雌两个基因池, 筛选了64对AFLP选择性引物EcoR I-NN +Mse I-NNN组合, 发现EcoR I-TG +Mse I-CAC引物组合在全雌基因池中扩增出一条分子量为234 bp的特异带。经F2 代单株验证, 该特异条带能在全雌单株中稳定出现。以MAP MAKER (Version 310) 软件分析, 该标记与全雌性位点的连锁距离在617 cM。命名该连锁标记为TG/CAC234。将该特异条带回收、克隆、测序, 设计特异SCAR引物, 再对F2 代单株基因组DNA进行扩增, 仅在全雌单株中扩增出1条分子量为166 bp 的特异带, 表明已成功地将与黄瓜全雌性连锁的AFLP标记转化为操作简便、表现稳定的SCAR标记, 该标记命名为SA166

关键词: 黄瓜, 全雌性, 分子标记, AFLP, SCAR

Abstract: Gynoecy plays an important role in cucumber (Cucumis sativus L. ) heterosis breeding and identification of the markers linked to this character will facilitate selection of gynoecious cucumber line in breeding p rogram. In the present study, selfed gynoecious cucumber‘Delta Star’and monoecious cucumber
‘Beijing Jietou’were used as parents to make F1 and then selfed to build F2 sex segregated population. Gynoecious and monoecious DNA pools were developed separately using bulked segregant analysis (BSA). Amplified fragment length polymorphism (AFLP) technique with 64 primer combinations were emp loyed to find the polymorphisms between both DNA pools, and in gyneocious DNA pool a 234 bp specific fragment was amplified in the primer combination of TG +CAC. This marker was testified with individual DNA of the F2 population and the band could only be amplified in the gynoecious plants. Linkage analysis using the software of MAPMAKER ( version 3.0) indicated its genetic distance to the gynoecious lociwas 617 cM, and this AFLP marker was designed as TG/CAC234. This band was collected and sequenced to synthesize a sequence-characterized amplified region (SCAR) primer. The primer was used to amplify the individual DNAs of the F2 population and obtained a specific band of 166 bp in the gynoecious plants, indicating a successful conversion of the SCAR marker from AFLP one. This SCAR markerwas designed as SA166 , and has been practically useful to the marker-assisted selection in our cucumber breeding program.

Key words: Cucumber (Cucumis sativus L. ), Gyneocious, Molecular marker, AFLP, SCAR