基于激素、转录组和代谢组研究木棉皮刺的遗传调控

doi:10.16420/j.issn.0513-353x.2024-0376

园艺学报 ›› 2025, Vol. 52 ›› Issue (4): 933-946.doi: 10.16420/j.issn.0513-353x.2024-0376

• 遗传育种·种质资源·分子生物学 • 上一篇下一篇

基于激素、转录组和代谢组研究木棉皮刺的遗传调控

邵一帆¹，朱宝庆¹，王童欣¹，廖建和²，吴繁花³，杨思怡¹，冯建行¹，于旭东^1,*(yuxd@hainanu.edu.cn)

¹海南大学热带农林学院，热带特色林木花卉遗传与种质创新教育部重点实验室，海南省热带特色花木资源生物学重点实验室，海南儋州 571737 ²海南大学材料科学与工程学院，海口 570228 ³海南大学生命科学学院，海口 570228

收稿日期:2025-01-08 修回日期:2025-02-20 出版日期:2025-04-25 发布日期:2025-04-25
通讯作者: ^*E-mail：yuxd@hainanu.edu.cn
基金资助:
海南省重点研发计划项目（ZDYF2021SHFZ258）；海南省重大科技计划项目（ZDKJ2021018）

Research of Genetic Regulation of Bombax ceiba Prickle Based on Hormone，Transcriptome and Metabolome

SHAO Yifan¹，ZHU Baoqing¹，WANG Tongxin¹，LIAO Jianhe²，WU Fanhua³，YANG Siyi¹，FENG Jianhang¹，and YU Xudong^1,*(yuxd@hainanu.edu.cn)

¹Key Laboratory of Genetics and Germplasm Innovation of Tropical Forest Trees and Ornamental Plants（Ministry of Education）；Key Laboratory of Germplasm Resources Biology of Tropical Special Ornamental Plants of Hainan Province，School of Tropical Agriculture and Forestry, Hainan University，Danzhou，Hainan 571737，China ²School of Materials Science and Engineering，Hainan University，Haikou 570228，China ³College of life sciences，Hainan University，Haikou 570228，China

Received:2025-01-08 Revised:2025-02-20 Published:2025-04-25 Online:2025-04-25

摘要/Abstract

摘要： 以木棉（Bombax ceiba）上部少刺区和基部多刺区茎皮为材料，通过植物激素测定、转录组学和代谢组学等方法，探究木棉皮刺形成的关键调控基因。激素测定发现，少刺区茎皮脱落酸、茉莉酸和水杨酸含量高于多刺区，而多刺区赤霉素含量高于少刺区。转录组测序发现，在有刺区，生长素生物合成通路中，Aux/IAA蛋白基因和生长素响应因子ARF基因的表达高于无刺区；细胞分裂素生物合成通路中 B-ARR基因表达高于无刺区；在无刺区，赤霉素生物合成通路中，GID1、DELLA和TF基因的表达高于有刺区；茉莉酸生物合成通路中，JAR1、JAZ和MYC2基因的表达高于有刺区。通过qRT-PCR验证了13个（BRI1、BZR1/2、AUX1、AUX/IAA、ARF、DELLA、JAR1、TGA、PR-1、SIMKK、MYB62、MYB123和WRKY28）影响木棉皮刺发育的关键基因，与高通量测序结果一致，表明RNA-Seq结果可靠。代谢组学筛选出了2 187个代谢物和92个差异代谢物，确定苯丙素生物合成通路中的CCR、COMT基因以及类黄酮生物合成途径中FLS基因与木棉皮刺形成有关；缬氨酸、亮氨酸和异亮氨酸的代谢通路中，代谢物（2S）-2–异丙基苹果酸和（2R,3S）-3–异丙基苹果酸以及类黄酮生物合成途径中的槲皮素参与木棉皮刺形成。

关键词: 木棉, 皮刺, 植物激素, 转录组, 代谢组

Abstract: Key regulatory genes that create prickles in Bombax ceiba were explored via phytohormone assays，transcriptomics，and metabolomics by use of the stem bark from the upper less prickly zone and lower prickly zone. The phytohormone assay revealed that abscisic acid，jasmonic acid，and salicylic acid were higher in the less prickly zone than in the prickly zone，whereas gibberellin was higher in the more prickly zone than in the less prickly zone. Transcriptome sequencing revealed that in the growth hormone biosynthesis pathway，the expression of Aux/IAA protein genes and growth hormone response factor ARF genes was higher in the prickly zone than in the less prickly zone. In the cytokinin biosynthesis pathway，the expression of B-ARR genes was higher in the prickly zone than in the less prickly zone whereas，in the gibberellin biosynthesis pathway，the expression of the GID1，DELLA，and TF genes was higher in the less prickly zone than in the prickly area. Furthermore，the expression of the JAR1，JAZ，and MYC2 genes was higher in the less prickly zone than in the prickly zone in the jasmonic acid biosynthesis pathway. Overall，13 key genes（BRI1，BZR1/2，AUX1，AUX/IAA，ARF，DELLA，JAR1，TGA，PR-1，SIMKK，MYB62，MYB123，and WRKY28） that affected the development of cottonwood prickles were verified by qRT-PCR，which were similar to the results of high-throughput sequencing；thus，supporting the reliability of the RNA-Seq results. Metabolomics screened 2 187 metabolites and 92 differential metabolites and identified that the CCR and COMT genes in the phenylpropanoid biosynthesis pathway as well as FLS genes in the flavonoid biosynthesis pathway, were related to the formation of cottonwood prickles. In addition，the metabolites（2S）-2-isopropylmalic acid and （2R,3S）-3-isopropyl malate in the valine，leucine，and isoleucine metabolic pathways and quercetin in the flavonoid biosynthesis pathway are involved in cottonwood prickle formation.

Key words: Bombax ceiba, prickle, plant hormones, transcriptome, metabolome

中图分类号:

S 685.99

邵一帆, 朱宝庆, 王童欣, 廖建和, 吴繁花, 杨思怡, 冯建行, 于旭东. 基于激素、转录组和代谢组研究木棉皮刺的遗传调控[J]. 园艺学报, 2025, 52(4): 933-946.

SHAO Yifan, ZHU Baoqing, WANG Tongxin, LIAO Jianhe, WU Fanhua, YANG Siyi, FENG Jianhang, YU Xudong. Research of Genetic Regulation of Bombax ceiba Prickle Based on Hormone，Transcriptome and Metabolome[J]. Acta Horticulturae Sinica, 2025, 52(4): 933-946.

参考文献

[1]	Boter M，Ruíz-Rivero O，Abdeen A，Prat S. 2004. Conserved MYC transcription factors play a key role in jasmonate signaling both in tomato and Arabidopsis. Genes & Development，18 (13)：1577–1591.
[2]	Fambrini M，Pugliesi C. 2019. The dynamic genetic-hormonal regulatory network controlling the trichome development in leaves. Plants-basel，8 (8)：253.
[3]	Feng Fayu，Wang Yi，Lu Bin，He Jiani，Yuan XiaoLong. 2022. Identification and expression analysis of Zanthoxylum armatum WRKY gene family based on the transcripome data. Molecular Plant Breeding，20 (18)：5984–5994. (in Chinese)
	冯发玉，王毅，陆斌，何家妮，原晓龙. 2022. 基于转录组数据竹叶花椒WRKY基因家族的鉴定及表达分析. 分子植物育种，20 (18)：5984–5994.
[4]	Feng Fayu，Wang Yi，Wang Lijuan，Romania，Xu Lingwen，Yang Yanping，Lu Bin. 2021. Identification and expression analysis of MYB gene family in Zanthoxylum armatum. Economic forest research，39 (4)：148–157. (in Chinese)
	冯发玉，王毅，王丽娟，罗玛妮娅，徐令文，杨彦萍，陆斌. 2021. 竹叶花椒MYB基因家族的鉴定及其表达特性的分析. 经济林研究，39 (4)：148–157.
[5]	Gan Y，Kumimoto R,Liu C，Ratcliffe O，Yu H，Broun P. 2006. GLABROUS INFLORESCENCE STEMS modulates the regulation by gibberellins of epidermal differentiation and shoot maturation in Arabidopsis. Plant Cell，18 (6)：1383–1395.
[6]	Guan Shuwen. 2022. Study on the molecular mechanism of prickly development of Zanthoxylum armatum[M. D. Dissertation]. Kunming：Southwest Forestry University. (in Chinese)
	关淑文. 2022. 竹叶花椒的皮刺发育分子机理研究[硕士论文]. 昆明：西南林业大学.
[7]	Hua B，Chang J，Xu Z，Han X，Xu M，Yang M，Yang C，Ye Z，Wu S. 2021. HOMEODOMAIN PROTEIN8 mediates jasmonate-triggered trichome elongation in tomato. New Phytologist，230 (3)：1063–1077.
[8]	Huang H，Liu B，Liu L，Song S. 2017. Jasmonate action in plant growth and development. Journal of Experimental Botany，68 (6)：1349–1359.
[9]	Li J，Wang X，Jiang R，Dong B，Fang S，Li Q，Lv Z，Chen W. 2021. Phytohormone-based regulation of trichome development. Frontiers in Plant Science，12：734776.
[10]	Li Xuhui，Chen Weiwei，Qi Yongwen，Lu Siqi. 2023. Application of maize ZmWRKY28 gene in regulating root hair length. CN116640773A，1–11. (in Chinese)
	李旭辉，陈卫卫，齐永文，陆思奇. 2023. 玉米ZmWRKY28基因在调控根毛长度中的应用. CN116640773A，1–11.
[11]	Liu Jinqiu，Chen Kai，Zhang Zhenzhu，Chen Xiuling，Wang Aoxue. 2016. Effects of exogenous GA，MeJA，IAA，SA and KT on trichome formation in tomato. Acta Horticulturae Sinica，43 (11)：2151–2160. (in Chinese)
	刘金秋，陈凯，张珍珠，陈秀玲，王傲雪. 2016. 外施GA、MeJA、IAA、SA和KT对番茄表皮毛发生的作用. 园艺学报，43 (11)：2151–2160.
[12]	Liu X，He X，Liu Z，Wu P，Tang N，Chen Z，Zhang W，Rao S，Cheng S，Luo C，Xu F. 2022. Transcriptome mining of genes in Zanthoxylum armatum revealed ZaMYB86 as a negative regulator of prickly development. Genomics，114 (3)：110374.
[13]	Pandey S，Goel R，Bhardwaj A，Asif M，H Sawant，S V，Misra P. 2018. Transcriptome analysis provides insight into prickle development and its link to defense and secondary metabolism in Solanum viarum dunal. Scientific Reports，11 (1)：16098.
[14]	Qi T，Huang H，Wu D，Yan J，Qi Y，Song S，Xie D.2014. Arabidopsis DELLA and JAZ proteins bind the WD-repeat/bHLH/MYB complex to modulate gibberellin and jasmonate signaling synergy. Plant Cell，26 (3)：1118–1133.
[15]	Ren Z，Li Z，Miao Q，Yang Y，Deng W，Hao Y. 2011. The auxin receptor homologue in Solanum lycopersicum stimulates tomato fruit set and leaf morphogenesis. Journal of Experimental Botany，62 (8)：2815–2826.
[16]	Tang N，Cao Z，Wu P，Liu Y，Lou J，Hu Y，Sun X，Si S，Chen Z. 2023. Comparative transcriptome analysis reveals hormone，transcriptional and epigenetic regulation involved in prickle formation in Zanthoxylum armatum. Gene，871：147434.
[17]	Traw M B，Bergelson J. 2003. Interactive effects of jasmonic acid，salicylic acid，and gibberellin on induction of trichomes in Arabidopsis. Plant Physiology，133 (3)：1367–1375.
[18]	Wang T，Jia Q，Wang W，Hussain S，Ahmed S，Adnan，Zhou，D X，Ni Z，Wang S. 2019. GCN5 modulates trichome initiation in Arabidopsis by manipulating histone acetylation of core trichome initiation regulator genes. Plant Cell Reports，38 (6)：755–765.
[19]	Werner S，Bartrina I，Schmülling T. 2021. Cytokinin regulates vegetative phase change in Arabidopsis thaliana through the miR172/TOE1-TOE2 module. Nature Communications，12 (1)：5816.
[20]	Wu Jinshan，Yu Xudong，Xu Zekang，Wang Yachen，Gao Haojie，Zhao Ying. 2017. Photosynthetic characteristics and carbon sink capacity of Hainan Bombax ceiba in different months. Tropical Agriculture in China，(3)：67–71. (in Chinese)
	吴金山，于旭东，许泽康，王亚沉，高豪杰，赵莹. 2017. 海南木棉不同月份光合特性及碳汇能力研究. 中国热带农业，(3)：67–71.
[21]	Wu Yansheng，Yan Wenpei，Zhang Xiaoli，Niu Weitao，Zhang Pengfei，Wu Lina，Li Min，Xing Cuijuan，Wang Senghu. 2021. Discrimination on the types and functions of plant thorns. Bulletin of Biology，56 (4)：13–15. (in Chinese)
	武延生，严文培，张晓丽，牛伟涛，张鹏飞，武丽娜，李敏，邢翠娟，王僧虎. 2021. 植物刺的类型与功能辨析. 生物学通报，56 (4)：13–15.
[22]	Xia X，Hu Q，Li W，Chen Y，Han L，Miao T，Wu W，Li X，Huang G. 2018. Cotton（Gossypium hirsutum） JAZ3 and SLR1 function in jasmonate and gibberellin mediated epidermal cell differentiation and elongation. Plant Cell Tissue and Organ Culture，133 (2)：1–14.
[23]	Xia Xiaocong. 2017. Study on the function of Gossypium hirsutum GhJAZ3 and GhGAI1 in JA and GA mediated trichome differentiation and elongation of transgenic Arabidopsis thaliana[M. D. Dissertation]. Wuhan：Central China Normal University. (in Chinese)
	夏小丛. 2017. 棉花（Gossypium hirsutum）GhJAZ3和GhGAI1在JA和GA介导的转基因拟南芥表皮毛分化和伸长中的功能研究[硕士论文]. 武汉：华中师范大学.
[24]	Yao Kexin. 2023. Functional study of the BsMYB62 transcription factor in Begonia semperflorens[M. D. Dissertation]. Zhengzhou：Henan Agricultural University. (in Chinese)
	姚珂心. 2023. 四季秋海棠BsMYB62转录因子的功能研究[硕士论文]. 郑州：河南农业大学.
[25]	Yu N，Cai W J，Wang S C，Shan C M，Wang L J，Chen X Y. 2010. Temporal control of trichome distribution by microRNA156-targeted SPL genes in Arabidopsis thaliana. Plant Cell，22 (7)：2322–2335.
[26]	Zhuang Yuan. 2015. Cloning and functional analysis of chalcone synthase gene and Cinnamoyl-CoA reductase gene in Pinus koraiensis[M. D. Dissertation]. Changchun：Jilin University. (in Chinese)
	庄园. 2015. 红松查尔酮合酶基因和肉桂酰辅酶A还原酶基因的克隆及功能分析[硕士论文]. 长春：吉林大学.

基于激素、转录组和代谢组研究木棉皮刺的遗传调控

Research of Genetic Regulation of Bombax ceiba Prickle Based on Hormone，Transcriptome and Metabolome

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

访问总数:　当日访问总数:　当前在线人数:

[1]	袁娟伟, 贾利, 王涵, 严从生, 张其安, 俞飞飞, 甘德芳, 江海坤. 辣椒种质资源疫病抗性鉴定及抗病基因挖掘[J]. 园艺学报, 2025, 52(4): 857-871.
[2]	唐伶俐, 贺玉花, 王庆涛, 安璐璐, 徐永阳, 张健, 孔维虎, 户克云, 赵光伟. 非呼吸跃变和呼吸跃变型甜瓜成熟果实的激素与转录组分析[J]. 园艺学报, 2025, 52(4): 883-896.
[3]	田玉凤, 马松亚, 杨安, 韩晓蕾, 张彩霞. 苹果砧木B9再生体系的建立与优化[J]. 园艺学报, 2025, 52(4): 947-958.
[4]	吝茹雪, 王斐, 张艳杰, 马力, 刘肖烽, 李舒然, 刘亚龙, 王晨, 姜淑苓, 欧春青. 梨矮生相关基因转录组筛选与功能分析[J]. 园艺学报, 2025, 52(3): 545-560.
[5]	李芮, 王稳, 杜明辉, 刘根忠, 马方放, 包志龙. SlBON1调控番茄植株营养生长机制的研究[J]. 园艺学报, 2025, 52(1): 73-87.
[6]	李洁, 武超, 贾祥堑, 王娟. ‘壶瓶枣’果皮着色物质及其相关基因筛选[J]. 园艺学报, 2024, 51(8): 1728-1742.
[7]	匡美美, 李黎, 马建伟, 刘原, 蒋鸿霏, 雷瑞, 满玉萍, 王一帆, 黄波, 王彦昌, 刘世彪. 利用中华猕猴桃杂交后代转录组测序筛选抗溃疡病相关基因[J]. 园艺学报, 2024, 51(8): 1743-1757.
[8]	韩荧, 段颖, 牛一杰, 李衍素, 贺超兴, 孙敏涛, 王君, 李强, 陈双臣, 闫妍. 腐殖酸生物降解地膜提高番茄品质的转录代谢机制研究[J]. 园艺学报, 2024, 51(8): 1758-1772.
[9]	段敏杰, 李怡斐, 王春萍, 杨小苗, 黄任中, 黄启中, 张世才. 辣椒果实类胡萝卜素调控因子转录组和靶向代谢组分析[J]. 园艺学报, 2024, 51(8): 1773-1791.
[10]	李文远, 林梦桦, 李亚辉, 于全琦, 梁颖, 张志勇. 梨果实代谢组学研究进展[J]. 园艺学报, 2024, 51(7): 1595-1609.
[11]	姚丰平, 王衍彬, 秦玉川, 王丽玲. 不同干燥处理的树参叶黄酮类成分的非靶向代谢组学分析[J]. 园艺学报, 2024, 51(5): 1069-1082.
[12]	张文昊, 张辉, 刘雨婷, 王艳, 张迎迎, 王馨曼, 王全华, 朱为民, 杨学东. 番茄含糖量不同的两个材料果实转录组初步分析[J]. 园艺学报, 2024, 51(2): 281-294.
[13]	卜文轩, 姚奕平, 黄宇, 杨星宇, 罗小宁, 张旻桓, 雷维群, 王政, 田珈宁, 陈露洁, 秦莉萍. ‘呼红’牡丹高温胁迫响应生理与转录组分析[J]. 园艺学报, 2024, 51(12): 2800-2816.
[14]	田洁, 周倩怡, 铁原毓, 孙海宏, 黄思杰. 干旱胁迫下大蒜幼苗的代谢组学分析[J]. 园艺学报, 2024, 51(1): 133-144.
[15]	徐旭华, 黄文洁, 陈旭峰, 陈园园, 吴绍文, 李红建, 晏石娟. ‘丹霞2号’红茶加工过程中品质特征成分的动态变化研究[J]. 园艺学报, 2024, 51(1): 145-161.

基于激素、转录组和代谢组研究木棉皮刺的遗传调控

Research of Genetic Regulation of Bombax ceiba Prickle Based on Hormone，Transcriptome and Metabolome

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

访问总数: 当日访问总数: 当前在线人数:

访问总数:　当日访问总数:　当前在线人数: