辣椒种质资源疫病抗性鉴定及抗病基因挖掘

doi:10.16420/j.issn.0513-353x.2024-0626

园艺学报 ›› 2025, Vol. 52 ›› Issue (4): 857-871.doi: 10.16420/j.issn.0513-353x.2024-0626

• 遗传育种·种质资源·分子生物学 • 上一篇下一篇

辣椒种质资源疫病抗性鉴定及抗病基因挖掘

袁娟伟^1,2，贾利²，王涵²，严从生²，张其安²，俞飞飞²，甘德芳^1,*(gandf@ahau.edu.cn)，江海坤^2,*(jhk211@163.com)

¹安徽农业大学园艺学院，合肥 230036 ²安徽省农业科学院蔬菜研究所，园艺作物种质创制及高效栽培安徽省重点实验室，农业农村部园艺作物种质创制与利用重点实验室，合肥 230031

收稿日期:2024-12-24 修回日期:2025-03-11 出版日期:2025-04-25 发布日期:2025-04-25
通讯作者:
^*E-mail：gandf@ahau.edu.cn,

jhk211@163.com
基金资助:
现代农业产业技术体系建设专项资助（CARS-23-G40，CARS-23-G49）；安徽省辣椒良种联合攻关项目；安徽省科技重大专项（202203a06020030）；安徽省农业科学院青年英才经费项目（QNYC-202121）；安徽省蔬菜产业技术体系专项经费项目（皖农科函[2021]711号）；安徽省自然科学研究重点项目（2023AH051056）

Screening of Resistant Germplasm and Identifying Candidate Gene for Phytophthora capsici Resistance in Pepper Germplasm Resources

YUAN Juanwei^1,2，JIA Li²，WANG Han²，YAN Congsheng²，ZHANG Qi’an²，YU Feifei²，GAN Defang^1,*(gandf@ahau.edu.cn)，and JIANG Haikun^2,*(jhk211@163.com)

¹College of Horticulture，Anhui Agricultural University，Hefei 230036，China ²Key Laboratory of Germplasm Creation and Utilisation of Horticultural Crops，Co-construction Ministry of Agriculture and Rural Affairs and Anhui Province，Key Laboratory of Genetic Improvement and Hingh-Efficiency Cultivation of Horticultural Crop，Institute of vegetable，Anhui Academy of Agricultural Sciences，Hefei 230031，China

Received:2024-12-24 Revised:2025-03-11 Published:2025-04-25 Online:2025-04-25

摘要/Abstract

摘要： 为筛选辣椒抗病种质并挖掘相关基因，利用12对疫病抗性SSR引物对214份辣椒种质材料进行初筛和复筛，得到多态性好的6对SSR抗性引物和56份疫病抗性辣椒种质。利用辣椒疫霉菌孢子悬浮液接种抗性辣椒种质并统计病情，其中11份为高抗。对接种后不同时期的辣椒茎部进行转录组测序，对差异表达基因进行GO、KEGG富集和WGCNA等分析，共筛选出15个抗病相关候选基因。

关键词: 辣椒, 疫霉菌, SSR分子标记, 转录组, 加权基因共表达网络分析, 候选基因

Abstract: In order to screen pepper germplasm for disease resistance and explore disease resistance related genes，12 pairs of SSR primers for disease resistance were used for initial and secondary screening of 214 pepper germplasm materials，resulting in 6 pairs of SSR resistant primers with good polymorphism and 56 pepper germplasm with disease resistance. Inoculate resistant pepper germplasm with pepper phytophthora spore suspension and record the disease status，among which 11 were highly resistant. Transcriptome sequencing was performed on pepper stems at different stages after inoculation，and GO，KEGG enrichment，and WGCNA analysis were performed on differentially expressed genes. A total of 15 candidate genes related to disease resistance were screened.

Key words: pepper, Phytophthora capsici, SSR molecular marker, transcriptome, WGCNA, candidate gene

中图分类号:

S 641.3

袁娟伟, 贾利, 王涵, 严从生, 张其安, 俞飞飞, 甘德芳, 江海坤. 辣椒种质资源疫病抗性鉴定及抗病基因挖掘[J]. 园艺学报, 2025, 52(4): 857-871.

YUAN Juanwei, JIA Li, WANG Han, YAN Congsheng, ZHANG Qi’an, YU Feifei, GAN Defang, JIANG Haikun. Screening of Resistant Germplasm and Identifying Candidate Gene for Phytophthora capsici Resistance in Pepper Germplasm Resources[J]. Acta Horticulturae Sinica, 2025, 52(4): 857-871.

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辣椒种质资源疫病抗性鉴定及抗病基因挖掘

Screening of Resistant Germplasm and Identifying Candidate Gene for Phytophthora capsici Resistance in Pepper Germplasm Resources

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辣椒种质资源疫病抗性鉴定及抗病基因挖掘

Screening of Resistant Germplasm and Identifying Candidate Gene for Phytophthora capsici Resistance in Pepper Germplasm Resources

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