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园艺学报 ›› 2023, Vol. 50 ›› Issue (11): 2435-2452.doi: 10.16420/j.issn.0513-353x.2022-0796

• 遗传育种·种质资源·分子生物学 • 上一篇    下一篇

素馨属种质资源形态特征与挥发物多样性研究

陈梅春1, 邓英杰2, 陈峥1, 朱育菁1, 李春牛3,*(), 王阶平1,*(), 刘波1   

  1. 1 福建省农业科学院农业生物资源研究所,福州 350003
    2 华中农业大学生命科学与技术学院,武汉 430070
    3 广西壮族自治区农业科学院花卉研究所,南宁 530007
  • 收稿日期:2023-05-30 修回日期:2023-08-11 出版日期:2023-11-25 发布日期:2023-11-28
  • 通讯作者:
    *(E-mail:
  • 基金资助:
    福建省农业科学院对外合作项目(DWHZ2021-12); “5511”协同创新工程项目(XTCXGC2021019)

Morphological Characteristics and Volatile Component Diversity of the Jasminum Germplasm Resources

CHEN Meichun1, DENG Yingjie2, CHEN Zheng1, ZHU Yujing1, LI Chunniu3,*(), WANG Jieping1,*(), LIU Bo1   

  1. 1 Agricultural Bioresources Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China
    2 College of life science and technology of Huazhong Agricultural University,Wuhan 430070,China
    3 Flower Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China

摘要:

利用固相微萃取—气质联用技术(SPME-GC-MS)测定了素馨属(Jasminum spp.)种质资源挥发性物质组成,结合聚类分析、主成分分析和香气评价指标对挥发性物质多样性及鲜花香气品质进行分析评价。结果表明:25份素馨属种质资源形态特征丰富,叶序为三叶轮生、互生羽状复叶或单叶对生;花色呈白色、白—粉双色或黄色;花冠有单层、双层和多层。25份种质资源中,大花素馨单花质量最小(0.10 g),虎头茉莉最大(2.41 g)。从25份素馨属种质资源中共鉴定出107个挥发性物质,包括43个萜类化合物、24个苯环类化合物、28个脂肪酸衍生物及12个含N-和NS-化合物。除大花素馨、毛萼素馨和福州双瓣茉莉外,其他22个种质资源的挥发性物质组成为首次报道。主成分和聚类分析显示25份素馨属种质资源聚为三大类群:类群Ⅰ包括浓香金茉莉,类群Ⅱ包括亮叶素馨、毛萼素馨和粉苞素馨,类群Ⅲ包括其余21个资源;经主成分分析,芳樟醇、苯甲醇、苯甲酸甲酯、乙酸苄酯、α-法呢烯、吲哚、茉莉酮和丁醛肟类香气等21个物质被筛选为差异特征成分。浓香金茉莉挥发性物质很少,含有极高的α-法呢烯(> 89%);丁醛肟类物质为毛萼素馨、亮叶素馨和粉苞素馨特有的关键成分,其他种质资源中均未检出;大花素馨形态特征与其他茉莉种明显不同,但其挥发性物质组成却相近。茉莉花香气品质评价表明,茉莉不同品种的鲜花香气品质存在差异,其中奥尔良少女、缅甸双瓣、泰国双瓣和台湾单瓣等香气品质不低于福州现有主栽品种‘双瓣茉莉’,可进行推广种植。

关键词: 素馨属, 茉莉花, 种质资源, 形态特征, 挥发性物质, 香气品质

Abstract:

The flower volatile compounds of Jasminum spp. were detected by using the SPME-GC-MS technique and diversity of flower volatile compounds and aroma quality were also evaluated by the cluster analysis,principal component analysis and aroma quality evaluation. The results showed that the 25 jasmine germplasm resources displayed abundant phenotypic traits:(i) the phyllotaxy of these jasmine germplasm resources was verticillate three-leaves,alternately pinnate compound leaves or opposite single-leaf;(ii) the flower color is white,white-pink bicolor or golden;and (iii) the corollas is single,double or multiple layer (s). Among the 25 germplasm resources,the lightest single flower mass was Jasminum grandiflorum(0.10 g),and the heaviest one was Jasminum sambac Hutou(2.41 g). Moreover,a total of 107 volatile components were identified from the 25 jasmine germplasm resources,including 43 Terpenoids,24 benzene compounds,28 fatty acid derivatives and 12 N- and NS-containing compounds. The volatile components of 22 germplasm resources were reported for the first time by this study,except for J. grandiflorumJ. multiflorum and Fuzhou double layers J. sambac. According to the results of the principal component and cluster analyses,the 25 jasmine germplasm resources could be clustered into three categories:Group Ⅰ,including J. odoratissimum;Group Ⅱ,including J. seguiniiJ. multiflorum and J. dichotomum;and Group Ⅲ,including the remaining 21 germplasms. Furthermore,21 substances including linalool,benzyl alcohol,methyl benzoate,benzyl acetate,α-farnesene,indole,jasmone and butyraldehyde oxime were screened as the differentially representative volatile components. In J. odoratissimum,few volatile components could be identified,but the content of α-farnesene was very high(> 89%). Butyraldehyde oxime-like substances were volatile components unique to group Ⅱ,and have not been detected in other jasmine germplasm resources. J. grandiflorum had significantly different phenotypic characteristics compared to J. Jasmine,while its volatile compositions were similar to J. Jasmine. The results of aroma quality evaluation indicated that aroma quality of different cultivars of J. sambac was significantly different. It is noteworthy that the aroma quality of Orleans girl,Myanmar double layers,Thailand double layers and Taiwan single layer was not lower than that of Fuzhou’s double layers,therefore,it is worth planting in a large area.

Key words: Jasminum spp., jasmine, germplasm resource, phenotypic characteristics, volatile component, aroma quality