茄子生长素响应因子SmARF5对分枝发育的影响

doi:10.16420/j.issn.0513-353x.2021-0700

园艺学报 ›› 2022, Vol. 49 ›› Issue (9): 1895-1906.doi: 10.16420/j.issn.0513-353x.2021-0700

茄子生长素响应因子SmARF5对分枝发育的影响

胡若琳¹, 王佳丽¹, 杨慧勤¹, 袁超¹, 牛义¹^,^*(), 汤青林¹, 魏大勇¹, 田时炳², 杨洋², 王志敏¹^,^*()

¹西南大学园艺园林学院,南方山地园艺学教育部重点实验室,重庆市蔬菜学重点实验室,重庆 400715
²重庆市农业科学院蔬菜花卉研究所,重庆 400055

收稿日期:2021-10-16 修回日期:2022-05-17 出版日期:2022-09-25 发布日期:2022-10-08
通讯作者: 牛义,王志敏 E-mail:niuy2001134@163.com;minzniwang_555@163.com
基金资助:
重庆市技术创新与应用发展专项重点项目(cstc2019jscx-gksbX0149);重庆市基础研究与前沿探索专项面上项目(cstc2019jcyj-msxmX0448);现代农业产业技术体系专项建设资金项目(CARS-23-A12);重庆市农业科学院绩效激励引导专项项目(cqaas2019jxj101)

Cloning and Functional Analysis of Auxin Response Factor Gene SmARF5 in Solanum melongena

HU Ruolin¹, WANG Jiali¹, YANG Huiqin¹, YUAN Chao¹, NIU Yi¹^,^*(), TANG Qinglin¹, WEI Dayong¹, TIAN Shibing², YANG Yang², WANG Zhimin¹^,^*()

¹Chongqing Key Laboratory of Olericulture,Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China
²The Institute of Vegetables and Flowers,Chongqing Academy of Agricultural Sciences,Chongqing 400055,China

Received:2021-10-16 Revised:2022-05-17 Online:2022-09-25 Published:2022-10-08
Contact: NIU Yi,WANG Zhimin E-mail:niuy2001134@163.com;minzniwang_555@163.com

摘要/Abstract

摘要：

以茄子（Solanum melongena）‘S12’为试验材料,同源克隆了生长素响应因子（auxin response factor ARF5）基因SmARF5,该基因开放阅读框全长2 793 bp,编码930个氨基酸,定位于细胞核。分别构建载体pGBKT7-SmARF5-Full和截短体pGBKT7-SmARF5-674aa进行酵母转化试验。结果表明,SmARF5具有转录激活活性,SmARF5全长可以与SmIAA16和SmIAA26蛋白互作,截短体SmARF5-674aa不与SmIAA16和SmIAA26互作。构建过表达载体pCAMBIA-2301G-SmARF5并转化入烟草。结果表明,与野生型相比,SmARF5转基因烟草植株出现明显分枝,茎粗增大。以上结果表明SmARF5可能通过生长素信号转导途径在植物分枝形成过程中发挥重要作用。

关键词: 茄子, SmARF5, 生长素, 分枝

Abstract:

SmARF5 gene was cloned from the eggplant cultivar‘S12’. The open reading frame is 2 793 bp,and it encodes a 930 aa protein that located in the nucleus. The pGBKT7-SmARF5-Full and pGBKT7-SmARF5-674aa vectors were constructed. The transcriptional activation activity of SmARF5 protein was analyzed and showed by yeast transformation. The full length of SmARF5 could interact with SmIAA16 and SmIAA26 proteins. The truncated SmARF5-674aa did not interact with SmIAA16 or SmIAA26. The pCAMBIA-2301G-SmARF5 overexpression vector was constructed and genetically transformed into tobacco plants. Compared with the wild type,obvious branches and the stem thickness were increased in the transgenic plants. The above results indicated that the SmARF5 transcription factor might play an important role in the formation of plant branches through the auxin signal transduction pathway.

Key words: Solanum melongena, SmARF5, auxin, branching

中图分类号:

S641.1

胡若琳, 王佳丽, 杨慧勤, 袁超, 牛义, 汤青林, 魏大勇, 田时炳, 杨洋, 王志敏. 茄子生长素响应因子SmARF5对分枝发育的影响[J]. 园艺学报, 2022, 49(9): 1895-1906.

HU Ruolin, WANG Jiali, YANG Huiqin, YUAN Chao, NIU Yi, TANG Qinglin, WEI Dayong, TIAN Shibing, YANG Yang, WANG Zhimin. Cloning and Functional Analysis of Auxin Response Factor Gene SmARF5 in Solanum melongena[J]. Acta Horticulturae Sinica, 2022, 49(9): 1895-1906.

图/表 9

表1 引物序列列表

Table 1 The list of primer sequence

用途 Purpose	引物 Primer	序列（5′-3′） Sequence（5′-3′）	限制酶 Restriction enzyme
全长克隆 Full-cloning	SmARF5-F SmARF5-R	ATGGGGTCTGTTGAAGTAAAGA GTGGAAGGCAAAGTTCAATAAAAGC
亚细胞定位 Subcellular localization	pCAMBIA1300-GFP-SmARF5-F pCAMBIA1300-GFP-SmARF5-R	CAAATCGACTCTAGAAAGCTTATGGGGTCTGTTGAAGTAAAGAACA GCCCTTGCTCACCATGGTACCGTTCGGAAATTCTGATGTTGAGC	HindⅢ KpnⅠ
遗传转化 Genetic transformation	SmARF5-2301G-F SmARF5-2301G-R	GCTCTAGAGCATGGGGTCTGTTGAAGTAAA CGAGCTCGTCAGTTCGGAAATTCTGATG	XbaⅠ SacⅠ
酵母双杂交 Yeast two- hybrid assay	pGADT7-SmARF5-F pGADT7-SmARF5-Full-R pGADT7-SmARF5-674aa-R pGBKT7-SmARF5-F pGBKT7-SmARF5-R pGBKT7-SmIAA16-F pGBKT7-SmIAA16-R pGBKT7-SmIAA26-F pGBKT7-SmIAA26-R	CGCCATATGATGGGGTCTGTTGAAGTAAAGAACA TCCCCCGGGTCAGTTCGGAAATTCTGATGTTGA CCGCTCGAGGCTATCTGCTACAGCTTGATAA CGCCATATGATGGGGTCTGTTGAAGTAAAGAACA TCCCCCGGGTCAGTTCGGAAATTCTGATGTTGA TCCCCCGGGAATGGTAGGGGAAGAAGAAA GCGTCGACTCAGTTAATTCTGTTCTTGC TCCCCCGGGAATGGAAGGTTATTCAAGAA GCGTCGACCTATGGCTGCTGCTTAGTTC	NdeⅠ SmaⅠ XhoⅠ NdeⅠ SmaⅠ SmaⅠ SalⅠ SmaⅠ SalⅠ
qRT-PCR	qRT-SmARF5-F qRT-SmARF5-R α-tubuin-F α-tubuin-R	ACTGTCCGTTGGTATGAGGT CAGGCCATCTCAGTGGATCC ATGAGAGAGTGCATATCGAT TTCACTGAAGAAGGTGTTGAA

图1 SmARF5基因在茄子基因组中的定位（A）和 SmARF5蛋白结构域（B）

Fig. 1 Location of SmARF5 gene in eggplant genome（A）and regional structures of SmARF5 proteins（B）

图2 不同物种ARF蛋白系统进化树

Fig. 2 Phylogenetic tree of ARF protein in different species

图3 茄子SmARF5蛋白的亚细胞定位

Fig. 3 Subcelluar localization of eggplant SmARF5 protein

图4 含重组诱饵质粒pGBKT7-SmARF5的酵母在SD/-Trp/X-α-gal培养基上呈蓝色（具转录活性）

Fig. 4 Yeast of recombinant bait plasmid pGBKT7-SmARF5 is blue on SD/-Trp/X-α-gal medium（transcriptionally active）

图5 SmARF5与SmIAA16和SmIAA26蛋白互作在四缺培养基上呈蓝色

Fig. 5 SmARF5 interacts with SmIAA16 and SmIAA26 proteins in blue on four-deficient medium

图6 SmARF5转基因烟草T0代的PCR检测 WT：野生型;D：ddH2O;P：重组质粒;1 ~ 16：转基因植株;M：Trans 2K plus DNA marker。

Fig. 6 PCR identification of T0 generation of SmARF5 transgenic tobacco WT：Wild type;D：ddH2O;P：Recombinant plasmid;1-16：Transgenic plants;M：Trans 2K plus DNA marker.

图7 SmARF5在野生型（WT）和转基因烟草（OE）中的表达量

Fig. 7 Expression of SmARF5 in wild type（WT）and transgenic tobacco（OE）

图8 烟草野生型（WT）和转基因植株（OE）的分枝表型和茎粗 * P < 0.05,** P < 0.01.

Fig. 8 Branching phenotype and stem diameter of tobacco wild-type（WT）and transgenic plants（OE）

参考文献 39

[1]	An Jianping, Song Laiqing, Zhao Lingling, You Chunxiang, Wang Xiaofei, Hao Yujin. 2018. Cloning and functional characterization of an auxin response factor gene MdARF5 in apple. Scientia Agricultura Sinica, 51 (7):1345-1352. (in Chinese)
	安建平, 宋来庆, 赵玲玲, 由春香, 王小非, 郝玉金. 2018. 苹果生长素响应因子MdARF5的克隆与功能鉴定. 中国农业科学, 51 (7):1345-1352.
[2]	Cao Zi-lin. 2019. Molecular mechanisms of endogenous hormone regulation in stump sprouting of Hippophae rhamnoides subspsinesis[M. D. Dissertation]. Beijing: Beijing Forestry University. (in Chinese)
	曹子林. 2019. 中国沙棘平茬萌蘖内源激素调控的分子机制[硕士论文]. 北京: 北京林业大学.
[3]	Ckurshumova W, Smirnova T, Marcos D, Zayed Y, Berleth T. 2014. Irrepressible MONOPTEROS/ARF 5 promotes de novo shoot formation. New Phytologist, 204 (3):556-566. doi: 10.1111/nph.13014 pmid: 25274430
[4]	Crawford S, Shinohara N, Sieberer T, Williamson L, George G, Hepworth J, Müller D, Domagalska M A, Leyser O. 2010. Strigolactones enhance competition between shoot branches by dampening auxin transport. Development, 137 (17):2905-2913. doi: 10.1242/dev.051987 pmid: 20667910
[5]	Domagalska M A, Leyser O. 2011. Signal integration in the control of shoot branching. Nature Reviews Molecular Cell Biology, 12 (4):211-221. doi: 10.1038/nrm3088 pmid: 21427763
[6]	Donner T J, Sherr I, Scarpella E. 2009. Regulation of preprocambial cell state acquisition by auxin signaling in Arabidopsis leaves. Development, 136 (19):3235-3246. doi: 10.1242/dev.037028 pmid: 19710171
[7]	Eduardo G G, Alice P, Josáíé M F Z, Carlos T, Richard G H I, Pilar C. 2017. Abscisic acid signaling is controlled by a BRANCHED1/HD-ZIP I cascade in Arabidopsis axillary buds. Proceedings of the National Academy of Sciences, 114 (2):E245-E254.
[8]	Feng Shuangshuang, Luo Jiayi, Zhu Xijian, Jiang Jibin, Huang Sanwen, Zhang Jinzhe. 2020. Homozygous mutant construction and function analysis of TCP transcription factor StBRC1a in diploid potato. Acta Horticulturae Sinica, 47 (1):63-72. (in Chinese)
	冯爽爽, 罗嘉翼, 朱曦鉴, 蒋继滨, 黄三文, 张金喆. 2020. 二倍体马铃薯StBRC1a功能缺失突变体的获得及其功能分析. 园艺学报, 47 (1):63-72.
[9]	Finlayson S A. 2007. Arabidopsis TEOSINTE BRANCHED1-LIKE 1 regulates axillary bud outgrowth and is homologous to monocot TEOSINTE BRANCHED1. Plant and Cell Physiology, 48 (5):667-677. pmid: 17452340
[10]	Fu Da-qi. 2016. Leaves syringe method//Operational guidance of virus-induced gene siliencing in plant. Beijing: China Agricultral University Press:52-57. (in Chinese)
	傅达奇. 2016. 叶片注射侵染法//植物中病毒诱导基因沉默技术操作指导. 北京: 中国农业大学出版社:52-57.
[11]	Gomez-Roldan V, Fermas S, Brewer P B, Puech-Pagès V, Dun E A, Pillot J P, Letisse F, Matusova R, Danoun S, Portais J C, Bouwmeester H, Bécard G, Beveridge C A, Rameau C, Rochange S F. 2008. Strigolactone inhibition of shoot branching. Nature, 455 (7210):89-194. doi: 10.1038/nature07252 URL
[12]	Gregor S, Klaus T. 2005. Shoot and inflorescence branching. Current Opinion in Plant Biology, 8 (5):506-511. pmid: 16054429
[13]	Guilfoyle T J, Hagen G. 2007. Auxin response factors. Current Opinion In Plant Biology, 10 (5):453-460. pmid: 17900969
[14]	Hardtke C S, Berleth T. 1998. The Arabidopsis gene MONOPTEROS encodes a transcription factor mediating embryo axis formation and vascular development. The EMBO journal, 17 (5):188-189.
[15]	Hu Yong-jun, Tian Su-bo, Li Xiao-gang. 2012. Eggplant multiple buds + lateral branch grafting technology. Science Planting and Raising,(11):27. (in Chinese)
	胡永军, 田素波, 李小刚. 2012. 茄子多芽 + 侧枝嫁接技术. 科学种养,(11):27.
[16]	Kenneth V T, Folke S. 1933. Studies on the growth hormone of plants:III. The inhibiting action of the growth substance on bud development. Proceedings of the National Academy of Sciences of the United States of America, 19:714-716.
[17]	Liu Congcong. 2017. The mechanisms of auxin and strigolactone control lateral branching outgrowth through cytokinin in tomato plants[M. D. Dissertation]. Hangzhou: Zhejiang University. (in Chinese)
	刘丛丛. 2017. 生长素及独脚金内酯通过细胞分裂素调控番茄侧枝生长发育的机制研究[硕士论文]. 杭州: 浙江大学.
[18]	Liu S Y, Zhang Y W, Feng Q S, Qin L, Pan C T, Lamin-Samu A T, Lu G. 2018. Tomato AUXIN RESPONSE FACTOR 5 regulates fruit set and development via the mediation of auxin and gibberellin signaling. Scientific Reports, 8 (1):2971. doi: 10.1038/s41598-018-21315-y pmid: 29445121
[19]	Liu Songyu, Yan Yanqiu, Feng Qiushuo, Lu Gang. 2018. Function analysis of SlARF12gene during fruit development in tomato. Acta Horticulturae Sinica, 45 (4):678-690. (in Chinese)
	刘松瑜, 闫艳秋, 冯秋硕, 卢钢. 2018. 番茄生长素响应因子基因SlARF12在果实发育过程中的功能分析. 园艺学报, 45 (4):678-690.
[20]	Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-∆∆CTmethod. Methods, 25:402-408. doi: 10.1006/meth.2001.1262 pmid: 11846609
[21]	McSteen P, Leyser O. 2005. Shoot branching. Annual Review of Plant Biology, 56:353-374. pmid: 15862100
[22]	Philip B B, Elizabeth A, Brett J. F, Catherine R, Christine A B. 2009. Strigolactone acts downstream of auxin to regulate bud outgrowth in pea and Arabidopsis. Plant Physiology, 150 (1):482-493. doi: 10.1104/pp.108.134783 pmid: 19321710
[23]	Snowden K C, Napoli C A. 2003. A quantitative study of lateral branching in petunia. Functional Plant Biology, 30 (9):987-994. doi: 10.1071/FP03081 pmid: 32689083
[24]	Shimizu-Sato S, Tanaka M, Mori H. 2009. Auxin-cytokinin interactions in the control of shoot branching. Plant Molecular Biology, 69 (4):429-435. doi: 10.1007/s11103-008-9416-3 pmid: 18974937
[25]	Sun Qian. 2020. Research on the function of strigolactones in the regulation of shoot branching in tomato[M. D. Dissertation]. Hangzhou: Zhejiang University. (in Chinese)
	孙倩. 2020. 独角金内酯调控番茄侧枝生长的功能研究[硕士论文]. 杭州: 浙江大学.
[26]	Thomas G, Oliver C, Elisabeth S, Dörte M, Rubén H, Gregor S, Klaus T. 2003. Molecular analysis of the LATERAL SUPPRESSOR gene in Arabidopsis reveals a conserved control mechanism for axillary meristemformation. Genes Development, 17 (9):1175-1187. doi: 10.1101/gad.260703 URL
[27]	Tiwari S B, Hagen G, Guilfoyle T. 2003. The roles of auxin response factor domains in auxin-responsive transcription. Plant Cell, 15 (2):533-543. pmid: 12566590
[28]	Tiwari S B, Wang X J, Hagen G, Guilfoyle T J. 2001. AUX/IAA proteins are active repressors,and their stability and activity are modulated by auxin. Plant Cell, 13 (12):2809-2822. pmid: 11752389
[29]	Ulmasov T, Murfett J, Hagen G, Guilfoyle T J. 1997. ARF1,a transcription factor that binds to auxin response elements. Science, 276 (5320):1865-1868. pmid: 9188533
[30]	Wang Min-xia. 2016. Functional analysis of rice(Orzya sativa L.)tillering regulator gene OsIAA16 and development of genetic modifiers of the d14 mutant[Ph. D. Dissertation]. Beijing:Chinese Academy of Agricultural Sciences. (in Chinese)
	王闵霞. 2016. 水稻分蘖调控基因OsIAA16的功能研究和d14突变体遗传修饰因子的创制[博士论文]. 北京: 中国农业科学院.
[31]	Woodward A W, Bonnie B. 2005. Auxin:regulation,action,and interaction. Ann Bot, 95 (5):707-735. doi: 10.1093/aob/mci083 URL
[32]	Xu C Z, Shen Y, He F, Fu X K, Yu H, Lu W X, Li Y L, Li C F, Fan D, Wang H C, Luo K M. 2019. Auxin-mediated Aux/IAA-ARF-HB signaling cascade regulates secondary xylem development in Populus. The New Phytologist, 222 (2):752-767. doi: 10.1111/nph.15658 URL
[33]	Xue Zhi-fei, Wang Xia, Li Fu-peng, Ma Chao-zhi. 2018. Homologous cloning of BnGS3 and BnGhd7 genes in Brassica napus and their relationship with rapeseed yield-related traits. Acta Agronomica Sinica, 44 (2):297-305. (in Chinese) doi: 10.3724/SP.J.1006.2018.00297 URL
	薛志飞, 王夏, 李付鹏, 马朝芝. 2018. 甘蓝型油菜BnGS3和BnGhd7的同源克隆及其与油菜产量相关性状的关系. 作物学报, 44 (2):297-305.
[34]	Yue Peng-tao. 2020. Auxin activated MdARF 5 induces the expression of ethylene biosynthetic genes to initiate apple fruit ripening[Ph. D. Dissertation]. Shenyang:Shenyang Agricultural University. (in Chinese)
	岳鹏涛. 2020. 生长素通过MdARF5诱导苹果(Malus domestica)果实乙烯合成及果实成熟的机理研究[博士论文]. 沈阳: 沈阳农业大学.
[35]	Zhang Jun,Qiu Shuang,He Jia-qi,Zhou Yu-ming,Wu Chang-le,Yuan Hong-miao,Liu Jia-yi,Yin Yi-jun,Shi Tong-rui,Zhai Ying. 2020. Construction of soybean GmGolS gene plant expression vector and tobacco genetic transformation. Journal of Qiqihar University (Natural Science Edition), 36 (6):22-25. (in Chinese)
	张军, 邱爽, 何佳琦, 周雨明, 邬长乐, 袁洪淼, 刘嘉仪, 尹伊珺, 史同瑞, 翟莹. 2020. 大豆GmGolS基因植物表达载体构建及烟草遗传转化. 齐齐哈尔大学学报(自然科学版), 36 (6):22-25.
[36]	Zhao C S, Craig J C, Petzold H E, Dickerman A W, Beers E P. 2005. The xylem and phloem transcriptomes from secondary tissues of the Arabidopsis root-hypocotyl. Plant Physiology, 138 (2):803-818. doi: 10.1104/pp.105.060202 URL
[37]	Zhao Z, Andersen S U, Ljung K, Dolezal K, Miotk A, Schultheiss S J, Lohmann J U. 2010. Hormonal control of the shoot stem-cell niche. Nature, 465 (7301):1089-1092. doi: 10.1038/nature09126 URL
[38]	Zhou Xi-meng, Fu Chun, Ma Chang-le, Wang Xing-jun, Zhao Chuan-zhi. 2021. Research progress of molecular regulation of branching of crops. Biotechnology Bulletin, 37 (3):107-114. (in Chinese) doi: 10.13560/j.cnki.biotech.bull.1985.2020-0906
	周希萌, 付春, 马长乐, 王兴军, 赵传志. 2021. 作物分枝的分子调控研究进展. 生物技术通报, 37 (3):107-114. doi: 10.13560/j.cnki.biotech.bull.1985.2020-0906
[39]	Zhu Gui-yuan. 2017. Molecular mechanisms study on the development of apical bud and vascular tissue regulated by PtSHRs gene[M. D. Dissertation]. Tianjin:Tianjin University. (in Chinese)
	祝桂媛. 2017. 杨树PtSHRs基因调控顶芽和维管组织发育的分子机制研究[硕士论文]. 天津: 天津大学.

茄子生长素响应因子SmARF5对分枝发育的影响

Cloning and Functional Analysis of Auxin Response Factor Gene SmARF5 in Solanum melongena

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茄子生长素响应因子SmARF5对分枝发育的影响

Cloning and Functional Analysis of Auxin Response Factor Gene SmARF5 in Solanum melongena

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