https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2022, Vol. 49 ›› Issue (4): 801-815.doi: 10.16420/j.issn.0513-353x.2021-0660

• 研究论文 • 上一篇    下一篇

香菇子实体发育过程中的转录组研究

沈楠, 张荆城, 王成晨, 边银丙, 肖扬*()   

  1. 华中农业大学应用真菌研究所,武汉 430070
  • 收稿日期:2021-08-26 修回日期:2022-03-21 出版日期:2022-04-25 发布日期:2022-04-24
  • 通讯作者: 肖扬 E-mail:xiaoyang@mail.hzau.edu.cn
  • 基金资助:
    国家重点研发项目子课题(2019YFD1001905-35);国家自然科学基金项目(32070015)

Studies on Transcriptome During Fruiting Body Development of Lentinula edodes

SHEN Nan, ZHANG Jingcheng, WANG Chengchen, BIAN Yinbing, XIAO Yang*()   

  1. Institute of Applied Mycology,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2021-08-26 Revised:2022-03-21 Online:2022-04-25 Published:2022-04-24
  • Contact: XIAO Yang E-mail:xiaoyang@mail.hzau.edu.cn

摘要:

对香菇子实体发育过程中不同阶段及组织(菌盖和菌柄)进行转录组测序,分析了差异表达基因、基因表达趋势、基因共表达网络及差异可变剪接事件。分析结果表明:在转色菌丝体阶段,大量碳水化合物代谢相关基因上调表达;此外,光受体等信号转导相关基因以及黑色素合成相关基因也上调表达,其可能参与到菌丝转色过程。原基形成过程可能涉及与环境因子感知、核糖体合成、真菌细胞壁重塑、蛋白质降解和染色质修饰等相关基因。与DNA复制和蛋白质降解相关的基因可能在菌盖发育过程中发挥重要作用,而糖苷水解酶基因可能在开伞过程中发挥作用。碳酸酐酶、几丁质酶、GH55家族蛋白和细胞色素P450编码基因可能在菌柄伸长过程中发挥作用。

关键词: 香菇, 子实体发育, 转录组, 时空表达, 共表达网络, 可变剪切

Abstract:

Samples from different stages and tissues(pileus and stipe of fruiting body)during the development of Lentinula edodes were collected and sequenced. By transcriptomic analyses,we identified differentially expressed genes. Then,gene expression trend,gene co-expression network and differentially alternative splicing event were analyzed. Results showed that a large number of CAZyme genes were up-regulated in mycelia browning. Genes associated with signal transduction,such as photoreceptors,and genes related to melanin biosynthesis may also be involved in mycelia browning. Primordium formation may be associated with genes involved in environmental factor sensing,ribosome biogenesis,fungal cell wall remodeling,protein degradation and chromatin modification. Genes associated with DNA replication and protein degradation may play important roles in pileus development,while genes encoding glycoside hydrolases may function in pileus opening. Genes encoding carbonic anhydrase,chitinases,GH55 and cytochrome P450s may serve the functions in stipe elongation.

Key words: Lentinula edodes, fruiting body development, transcriptome, spatiotemporal expression, co-expression network, alternative splicing

中图分类号: