园艺学报 ›› 2022, Vol. 49 ›› Issue (2): 265-280.doi: 10.16420/j.issn.0513-353x.2020-1000

• 研究论文 • 上一篇    下一篇


黎春红1,2, 汪开拓1,2,*(), 雷长毅1, 许凤3, 季娜娜2, 蒋永波1   

  1. 1重庆三峡学院生命科学与工程学院,重庆 404000
    2南京农业大学食品科技学院,南京 210095
    3宁波大学食品科学与工程系,浙江宁波 315211
  • 收稿日期:2021-10-30 修回日期:2022-01-18 出版日期:2022-02-25 发布日期:2022-02-28
  • 通讯作者: 汪开拓
  • 基金资助:

Identification of TGA Gene Family in Peach and Analysis of Expression Mode Involved in a BABA-Induced Disease Resistance

LI Chunhong1,2, WANG Kaituo1,2,*(), LEI Changyi1, XU Feng3, JI Nana2, JIANG Yongbo1   

  1. 1College of Life Science and Engineering,Chongqing Three Gorges University,Chongqing 404000,China
    2College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China
    3College of Food and Pharmaceutical Sciences,Ningbo University,Ningbo,Zhejiang 315211,China
  • Received:2021-10-30 Revised:2022-01-18 Online:2022-02-25 Published:2022-02-28
  • Contact: WANG Kaituo


以高度保守的BRLZ(PF07716)和DOG1(PF14144)结构域为种子序列,通过生物信息分析共鉴定得到15个桃TGA(TGACG motif-binding factor)家族基因,这些基因分布于桃的4条染色体上,其编码蛋白大小介于333 ~ 546 aa,分子量介于37.07 ~ 61.47 kD,等电点介于6.01 ~ 8.59,均定位于细胞核上。根据系统进化关系,拟南芥、大豆、番茄、水稻和桃的 TGA 家族分为5 个亚族,其中桃 TGA 家族成员主要分布于第Ⅰ、Ⅱ和Ⅳ亚族。对桃TGA家族成员启动子区域的顺式调控元件进行预测分析,其启动子区含有至少1个激素或逆境胁迫响应元件。经RNA-seq 数据分析可知,β-氨基丁酸(β- aminobutyric acid,BABA)处理和匍枝根霉(Rhizopus stolonifer)侵染能诱导桃TGA成员表达,其中PpTGA1-1在处理后12 h内上调最为显著;显著表达的PpTGA1-1通过与PpNPR1蛋白相互作用赋予PpNPR1蛋白DNA结合功能,启动一系列病程相关(pathogenesis-related,PR)基因的表达,从而诱导果实敏化(priming)抗性。桃 TGA 家族成员(尤其PpTGA1-1)可直接响应激发子诱导和病原菌侵染,并通过修饰PpNPR1蛋白从而在防卫反应中发挥重要调控作用。

关键词: 桃, TGA, 转录因子, β-氨基丁酸, 诱导抗病, 敏化, 软腐病


The highly conserved amino acid sequences of BRLZ(PF07716)and DOG1(PF14144)domains were used to identify the TGA family members,and the results showed that 15 members of the TGA family from the peach genome could be identified through bioinformatics analysis. The TGAs were unevenly distributed on the 1st,2rd,6th and 7th Prunus persica chromosomes. The protein parameters of the determined TGA TFs listed as follows:the sizes ranging from 333 to 546 aa,molecular weights ranging from 37.07 to 61.47 kD,the isoelectric points ranging from 6.01 to 8.59,respectively. And all members of the TGA family were located in the nucleus. According to the phylogenetic relationship, the TGA family of Arabidopsis thaliana,Glycine max,Solanum lycopersicum,Oryza sativa and Prunus persica was divided into five subfamilies,among which the peach TGA family members were mainly distributed inⅠ,Ⅱ and Ⅳ clades. Moreover,the promoter regions of the TGA members contained at least one plant hormone or stress response-related elements. In addition,the RNA-sequencing data exhibited that the expressions of the 15 TGA family members were potentiated by BABA treatment and/or Rhizopus stolonifer infection,and PpTGA1-1 expression was rapidly up-regulated within 12 h after stimulation. Further investigation indicated that the physical interaction between PpTGA1-1 and PpNPR1 provided the DNA binding capacity required by PpNPR1 for its activation of pathogenesis-related genes(PR)and consequently induced the priming resistance in harvested peaches. Collectively,a distinct member of TGA gene family in Prunus persica,particularly in PpTGA1-1,directly modified the PpNPR1 and thus exerted a crucial regulatory function for defensive response.

Key words: Prunus persica, TGA, transcription factor, β-aminobutyric acid, induced resistance, priming, Rhizopus rot