园艺学报 ›› 2022, Vol. 49 ›› Issue (1): 221-232.doi: 10.16420/j.issn.0513-353x.2020-0976
张爱萍1, 刘江娜1, 闫建俊2, 张西英1, 白云凤2()
收稿日期:
2021-02-19
修回日期:
2021-08-06
出版日期:
2022-01-25
发布日期:
2022-01-24
基金资助:
ZHANG Aiping1, LIU Jiangna1, YAN Jianjun2, ZHANG Xiying1, BAI Yunfeng2()
Received:
2021-02-19
Revised:
2021-08-06
Online:
2022-01-25
Published:
2022-01-24
摘要:
介绍了基因编辑技术的发展和现状,并对该技术在番茄耐贮性和成熟调控、营养成分和果色、雄性不育和单性结实、逆境响应、株形、花形及野生种重新驯化等方面的研究进展进行了概述,梳理了基于CRISPR/Cas技术的单碱基编辑、DNA-free编辑及提高同源重组效率的技术创新及在番茄遗传改良和品种培育方面的应用前景和面临的挑战。
中图分类号:
张爱萍, 刘江娜, 闫建俊, 张西英, 白云凤. 番茄基因编辑研究进展和前景[J]. 园艺学报, 2022, 49(1): 221-232.
ZHANG Aiping, LIU Jiangna, YAN Jianjun, ZHANG Xiying, BAI Yunfeng. Progress and Prospect of Genome Editing in Tomato[J]. Acta Horticulturae Sinica, 2022, 49(1): 221-232.
性状 Trait | 靶基因 Target gene | 基因编辑技术 Genome editing technology | 修复途径 Repair pathway | 参考文献 Reference |
---|---|---|---|---|
耐贮性/成熟调控 | Alc | CRISPR/Cas9 | NHEJ,HR | Yu et al., |
Storage / ripening | RIN | CRISPR/Cas9 | NHEJ | Ito et al., |
SP5G | CRISPR/Cas9 | NHEJ | Soyk et al., | |
α-Man | CRISPR/Cas9 | NHEJ | 张圆圆 等, | |
lncRNA1459 | CRISPR/Cas9 | NHEJ | Li et al., | |
ORRM4 | CRISPR/Cas9 | NHEJ | Yang et al., | |
果实营养 | GAD2/GAD3 | CRISPR/Cas9 | NHEJ | Nonaka et al., |
Fruit nutrition | GABA-T/SSADH | CRISPR/Cas9 | NHEJ | Li et al., |
SGR1/LCY-E/Blc/LCY-B1/LCY-B2 | CRISPR/Cas9 | NHEJ | Li et al., | |
果色Fruit color | MYB12 | CRISPR/Cas9 | NHEJ | Deng et al., |
PSY1/ CRTISO | CRISPR/Cas9 | NHEJ,HR | Dahan-Meir et al., | |
雄性不育 | STR1 | CRISPR/Cas9 | NHEJ | Du et al., |
Male sterility | AP3 | CRISPR/Cas9 | NHEJ | 刘玉琛 等, |
MPK20 | CRISPR/Cas9 | NHEJ | Chen et al., | |
单性结实 | AGL6 | CRISPR/Cas9 | NHEJ | Klap et al., |
Parthenocarpy | IAA9 | CRISPR/Cas9 | NHEJ | Ueta et al., |
生物胁迫反应 | Mlo1 | CRISPR/Cas9 | NHEJ | Nekrasov et al., |
Biotic stress reponse | JAZ 2 | CRISPR/Cas9 | NHEJ | Ortigosa et al., |
非生物胁迫反应 | PROCERA | TALEN | NHEJ | Lor et al., |
Abiotic stress reponse | MAPK3 | CRISPR/Cas9 | NHEJ | Wang et al., |
NPR1 | CRISPR/Cas9 | NHEJ | Li et al., | |
CBF1 | CRISPR/Cas9 | NHEJ | Li et al., | |
株型Plant architecture | J2/EJ2/LIN | CRISPR/Cas9 | NHEJ | Soyk et al., |
AGO7 | CRISPR/Cas9 | NHEJ | Brooks et al., | |
CLV3 | CRISPR/Cas9 | NHEJ | Rodríguez-Leal et al., | |
株色Plant color | ANT1 | TALEN,CRISPR/Cas9 | HR | Cermak et al., |
花序Inflorescence | L1L4 | ZFN | NHEJ | Hilioti et al., |
RRA3a | CRISPR/Cas9 | NHEJ | Xu et al., | |
BOP | CRISPR/Cas9 | NHEJ | Xu et al., |
表1 基因编辑技术对番茄栽培种性状的改良
Table 1 Application of genome editing technology in cultivated tomatoes
性状 Trait | 靶基因 Target gene | 基因编辑技术 Genome editing technology | 修复途径 Repair pathway | 参考文献 Reference |
---|---|---|---|---|
耐贮性/成熟调控 | Alc | CRISPR/Cas9 | NHEJ,HR | Yu et al., |
Storage / ripening | RIN | CRISPR/Cas9 | NHEJ | Ito et al., |
SP5G | CRISPR/Cas9 | NHEJ | Soyk et al., | |
α-Man | CRISPR/Cas9 | NHEJ | 张圆圆 等, | |
lncRNA1459 | CRISPR/Cas9 | NHEJ | Li et al., | |
ORRM4 | CRISPR/Cas9 | NHEJ | Yang et al., | |
果实营养 | GAD2/GAD3 | CRISPR/Cas9 | NHEJ | Nonaka et al., |
Fruit nutrition | GABA-T/SSADH | CRISPR/Cas9 | NHEJ | Li et al., |
SGR1/LCY-E/Blc/LCY-B1/LCY-B2 | CRISPR/Cas9 | NHEJ | Li et al., | |
果色Fruit color | MYB12 | CRISPR/Cas9 | NHEJ | Deng et al., |
PSY1/ CRTISO | CRISPR/Cas9 | NHEJ,HR | Dahan-Meir et al., | |
雄性不育 | STR1 | CRISPR/Cas9 | NHEJ | Du et al., |
Male sterility | AP3 | CRISPR/Cas9 | NHEJ | 刘玉琛 等, |
MPK20 | CRISPR/Cas9 | NHEJ | Chen et al., | |
单性结实 | AGL6 | CRISPR/Cas9 | NHEJ | Klap et al., |
Parthenocarpy | IAA9 | CRISPR/Cas9 | NHEJ | Ueta et al., |
生物胁迫反应 | Mlo1 | CRISPR/Cas9 | NHEJ | Nekrasov et al., |
Biotic stress reponse | JAZ 2 | CRISPR/Cas9 | NHEJ | Ortigosa et al., |
非生物胁迫反应 | PROCERA | TALEN | NHEJ | Lor et al., |
Abiotic stress reponse | MAPK3 | CRISPR/Cas9 | NHEJ | Wang et al., |
NPR1 | CRISPR/Cas9 | NHEJ | Li et al., | |
CBF1 | CRISPR/Cas9 | NHEJ | Li et al., | |
株型Plant architecture | J2/EJ2/LIN | CRISPR/Cas9 | NHEJ | Soyk et al., |
AGO7 | CRISPR/Cas9 | NHEJ | Brooks et al., | |
CLV3 | CRISPR/Cas9 | NHEJ | Rodríguez-Leal et al., | |
株色Plant color | ANT1 | TALEN,CRISPR/Cas9 | HR | Cermak et al., |
花序Inflorescence | L1L4 | ZFN | NHEJ | Hilioti et al., |
RRA3a | CRISPR/Cas9 | NHEJ | Xu et al., | |
BOP | CRISPR/Cas9 | NHEJ | Xu et al., |
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