番茄基因编辑研究进展和前景

doi:10.16420/j.issn.0513-353x.2020-0976

摘要/Abstract

摘要：

介绍了基因编辑技术的发展和现状,并对该技术在番茄耐贮性和成熟调控、营养成分和果色、雄性不育和单性结实、逆境响应、株形、花形及野生种重新驯化等方面的研究进展进行了概述,梳理了基于CRISPR/Cas技术的单碱基编辑、DNA-free编辑及提高同源重组效率的技术创新及在番茄遗传改良和品种培育方面的应用前景和面临的挑战。

关键词: 基因编辑, 番茄, 应用, 前景

Abstract:

In this review,we reviewed the recent progresses of the genome editing technology and its application in tomato. We summarized successful examples in fruit storage tolerance and ripening regulation,nutrient composition and fruit color,male sterility and parthenocarpy,stress response,plant architecture,inflorescencetype and de novo domestication of wild tomato species. We further stated the technical innovations of the CRISPR/Cas-based genome editing,such as single-base editing,DNA-free editing and the improvement of homologous recombination efficiency. We finally showed the prospects and challenges in applying the genome editing technology in tomato genetic improvement and new variety breeding.

Key words: genome editing, tomato, utilization, prospect

中图分类号:

S641.2

张爱萍, 刘江娜, 闫建俊, 张西英, 白云凤. 番茄基因编辑研究进展和前景[J]. 园艺学报, 2022, 49(1): 221-232.

ZHANG Aiping, LIU Jiangna, YAN Jianjun, ZHANG Xiying, BAI Yunfeng. Progress and Prospect of Genome Editing in Tomato[J]. Acta Horticulturae Sinica, 2022, 49(1): 221-232.

图/表 1

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性状 Trait	靶基因 Target gene	基因编辑技术 Genome editing technology	修复途径 Repair pathway	参考文献 Reference
耐贮性/成熟调控	Alc	CRISPR/Cas9	NHEJ,HR	Yu et al.,2017
Storage / ripening	RIN	CRISPR/Cas9	NHEJ	Ito et al.,2015
	SP5G	CRISPR/Cas9	NHEJ	Soyk et al.,2017b
	α-Man	CRISPR/Cas9	NHEJ	张圆圆等,2019
	lncRNA1459	CRISPR/Cas9	NHEJ	Li et al.,2018a
	ORRM4	CRISPR/Cas9	NHEJ	Yang et al.,2017
果实营养	GAD2/GAD3	CRISPR/Cas9	NHEJ	Nonaka et al.,2017
Fruit nutrition	GABA-T/SSADH	CRISPR/Cas9	NHEJ	Li et al.,2018b
	SGR1/LCY-E/Blc/LCY-B1/LCY-B2	CRISPR/Cas9	NHEJ	Li et al.,2018e
果色Fruit color	MYB12	CRISPR/Cas9	NHEJ	Deng et al.,2018
	PSY1/ CRTISO	CRISPR/Cas9	NHEJ,HR	Dahan-Meir et al.,2018
雄性不育	STR1	CRISPR/Cas9	NHEJ	Du et al.,2020
Male sterility	AP3	CRISPR/Cas9	NHEJ	刘玉琛等,2019
	MPK20	CRISPR/Cas9	NHEJ	Chen et al.,2018
单性结实	AGL6	CRISPR/Cas9	NHEJ	Klap et al.,2017
Parthenocarpy	IAA9	CRISPR/Cas9	NHEJ	Ueta et al.,2017
生物胁迫反应	Mlo1	CRISPR/Cas9	NHEJ	Nekrasov et al.,2017
Biotic stress reponse	JAZ 2	CRISPR/Cas9	NHEJ	Ortigosa et al.,2018
非生物胁迫反应	PROCERA	TALEN	NHEJ	Lor et al.,2014
Abiotic stress reponse	MAPK3	CRISPR/Cas9	NHEJ	Wang et al.,2017
	NPR1	CRISPR/Cas9	NHEJ	Li et al.,2019a
	CBF1	CRISPR/Cas9	NHEJ	Li et al.,2018c
株型Plant architecture	J2/EJ2/LIN	CRISPR/Cas9	NHEJ	Soyk et al.,2017a
	AGO7	CRISPR/Cas9	NHEJ	Brooks et al.,2014
	CLV3	CRISPR/Cas9	NHEJ	Rodríguez-Leal et al.,2017
株色Plant color	ANT1	TALEN,CRISPR/Cas9	HR	Cermak et al.,2015
花序Inflorescence	L1L4	ZFN	NHEJ	Hilioti et al.,2016
	RRA3a	CRISPR/Cas9	NHEJ	Xu et al.,2015
	BOP	CRISPR/Cas9	NHEJ	Xu et al.,2016

性状 Trait	靶基因 Target gene	基因编辑技术 Genome editing technology	修复途径 Repair pathway	参考文献 Reference
耐贮性/成熟调控	Alc	CRISPR/Cas9	NHEJ,HR	Yu et al.,2017
Storage / ripening	RIN	CRISPR/Cas9	NHEJ	Ito et al.,2015
	SP5G	CRISPR/Cas9	NHEJ	Soyk et al.,2017b
	α-Man	CRISPR/Cas9	NHEJ	张圆圆等,2019
	lncRNA1459	CRISPR/Cas9	NHEJ	Li et al.,2018a
	ORRM4	CRISPR/Cas9	NHEJ	Yang et al.,2017
果实营养	GAD2/GAD3	CRISPR/Cas9	NHEJ	Nonaka et al.,2017
Fruit nutrition	GABA-T/SSADH	CRISPR/Cas9	NHEJ	Li et al.,2018b
	SGR1/LCY-E/Blc/LCY-B1/LCY-B2	CRISPR/Cas9	NHEJ	Li et al.,2018e
果色Fruit color	MYB12	CRISPR/Cas9	NHEJ	Deng et al.,2018
	PSY1/ CRTISO	CRISPR/Cas9	NHEJ,HR	Dahan-Meir et al.,2018
雄性不育	STR1	CRISPR/Cas9	NHEJ	Du et al.,2020
Male sterility	AP3	CRISPR/Cas9	NHEJ	刘玉琛等,2019
	MPK20	CRISPR/Cas9	NHEJ	Chen et al.,2018
单性结实	AGL6	CRISPR/Cas9	NHEJ	Klap et al.,2017
Parthenocarpy	IAA9	CRISPR/Cas9	NHEJ	Ueta et al.,2017
生物胁迫反应	Mlo1	CRISPR/Cas9	NHEJ	Nekrasov et al.,2017
Biotic stress reponse	JAZ 2	CRISPR/Cas9	NHEJ	Ortigosa et al.,2018
非生物胁迫反应	PROCERA	TALEN	NHEJ	Lor et al.,2014
Abiotic stress reponse	MAPK3	CRISPR/Cas9	NHEJ	Wang et al.,2017
	NPR1	CRISPR/Cas9	NHEJ	Li et al.,2019a
	CBF1	CRISPR/Cas9	NHEJ	Li et al.,2018c
株型Plant architecture	J2/EJ2/LIN	CRISPR/Cas9	NHEJ	Soyk et al.,2017a
	AGO7	CRISPR/Cas9	NHEJ	Brooks et al.,2014
	CLV3	CRISPR/Cas9	NHEJ	Rodríguez-Leal et al.,2017
株色Plant color	ANT1	TALEN,CRISPR/Cas9	HR	Cermak et al.,2015
花序Inflorescence	L1L4	ZFN	NHEJ	Hilioti et al.,2016
	RRA3a	CRISPR/Cas9	NHEJ	Xu et al.,2015
	BOP	CRISPR/Cas9	NHEJ	Xu et al.,2016