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园艺学报 ›› 2021, Vol. 48 ›› Issue (12): 2458-2470.doi: 10.16420/j.issn.0513-353x.2021-0382

• 研究论文 • 上一篇    下一篇

铁皮石斛蛋白磷酸酶PP2C家族基因鉴定及其表达分析

张婷婷1, 李雨欣1, 张德遥1, 康宇乾1, 王健2, 宋希强2, 周扬1,*()   

  1. 1海南大学园艺学院,海南省热带园艺作物品质调控重点实验室,海南省耐盐作物生物技术重点实验室,海口 570228
    2海南大学林学院,热带特色林木花卉遗传与种质创新教育部重点实验室,海南省热带特色花木资源生物学重点实验室,海口 570228
  • 收稿日期:2021-07-20 修回日期:2021-08-26 发布日期:2022-01-04
  • 通讯作者: 周扬 E-mail:zhouyang@hainanu.edu.cn
  • 基金资助:
    海南省自然科学基金项目(319MS009);海南省自然科学基金项目(318QN189);海南省教育厅项目(Hys2020-242);海南省教育厅项目(Hnky2021-19);海南大学科研启动项目(KYQD(ZR)1845);海南省自然科学基金创新团队项目(2018CXTD331)

Genome-Wide Identification and Expression Analyses of PP2C Gene Family in Dendrobium catenatum

ZHANG Tingting1, LI Yuxin1, ZHANG Deyao1, KANG Yuqian1, WANG Jian2, SONG Xiqiang2, ZHOU Yang1,*()   

  1. 1Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province,Hainan Key Laboratory for Biotechnology of Salt Tolerant Crops,School of Horticulture,Hainan University,Haikou 570228,China
    2Key Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants/Key Laboratory of Germplasm Resources Biology of Tropical Special Ornamental Plants of Hainan Province,School of Forestry,Hainan University,Haikou 570228,China
  • Received:2021-07-20 Revised:2021-08-26 Published:2022-01-04
  • Contact: ZHOU Yang E-mail:zhouyang@hainanu.edu.cn

摘要:

利用生物信息学方法对铁皮石斛(Dendrobium catenatum Lindl.)2C型蛋白磷酸酶(protein phosphatase 2C,PP2C)家族基因进行鉴定分析,采用实时荧光定量PCR技术分析PP2C基因的组织表达特性,以及在不同非生物胁迫和植物生长调节剂处理下的表达模式。共鉴定出67个家族成员,其编码的氨基酸在80 ~ 1 075 aa之间,分子量在8.86 ~ 119.59 kD之间,等电点在4.13 ~ 9.36之间。进化树分析可将其分为13个亚族,且每个亚族蛋白的保守基元相似。基因结构分析表明,PP2C基因的外显子数量为1 ~ 15不等。空间表达模式分析发现有4个PP2C在铁皮石斛合蕊柱中表达量最高,5个基因在蒴果中表达量最高,有1个基因在根中表达量最高,PP2C的表达具有组织特异性。DcPP2C5、DcPP2C20DcPP2C56表达量受干旱胁迫(20% PEG6000)上调较明显,DcPP2C24受高温(42 ℃)诱导最显著,DcPP2C5受低温(4 ℃)诱导上调表达,DcPP2C20受到盐胁迫(200 mmol · L-1 NaCl)诱导,这些基因的表达均在不同程度上受100 μmol · L-1ABA和20 μmol · L-1 SA的诱导,说明它们在铁皮石斛应对逆境胁迫中发挥作用。

关键词: 铁皮石斛, 蛋白磷酸酶PP2C, 非生物胁迫, 植物生长调节剂, 表达分析

Abstract:

The protein phosphatase 2C(PP2C)family genes of Dendrobium catenatum Lindl. were identified and analyzed by bioinformatics method. The expression patterns of PP2C genes in different tissues and under various abiotic stresses and hormones were analyzed by quantitative real-time PCR (qRT-PCR). A total of 67 family members were identified,which encode 80 to 1 075 amino acids,and the molecular weight were between 8.86 to 119.59 kD,and the isoelectric points ranged from 4.13 to 9.36. The PP2C proteins were divided into 13 subgroups by phylogenetic tree analysis,and the conserved motifs of each subgroup were similar. The number of exons in PP2C gene ranged from 1 to 15. Spatial expression pattern analysis showed that four PP2Cs had the highest expression in gynostemium of D. catenatum,and five genes had the highest expression in capsule and one gene had the highest expression in root,indicating that the expression of PP2C was tissue-specific. The expression levels of DcPP2C5,DcPP2C20 and DcPP2C56 were significantly up-regulated by drought stress(20% PEG6000). DcPP2C24 was significantly up-regulated by high temperature(42 ℃). And DcPP2C5 was up-regulated by low temperature(4 ℃). DcPPC20 was induced by salt stress(200 mmol · L-1 NaCl), and the expression of these genes were all induced by 100 μmol · L-1 ABA and 20 μmol · L-1 SA to some degrees,suggesting that they played roles in response to the stresses in D. catenatum.

Key words: Dendrobium catenatum, protein phosphatase 2C, abiotic stress, plant growth regulator, expression analysis

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