GSH/GSSG在植物应对非生物胁迫中的作用综述

doi:10.16420/j.issn.0513-353x.2020-0589

摘要/Abstract

摘要：

谷胱甘肽（GSH）是广泛存在于植物体内的一种重要的抗氧化物质,可以清除细胞代谢中产生的多余活性氧自由基,从而减少膜脂过氧化对细胞造成的伤害。谷胱甘肽二硫化物（GSSG）是GSH的氧化形式。近年来研究发现谷胱甘肽氧化还原对（GSH/GSSG）作为信号分子在植物应对逆境胁迫中起重要作用。本文综述了近年来GSH,尤其是 GSH/GSSG的作用,为后续通过调控GSH/GSSG调节植物抗性提供参考。

关键词: 谷胱甘肽, 非生物胁迫, 信号传导, 防御机制

Abstract:

Glutathione（GSH）is an important antioxidant substance that widely exists in plants. It can eliminate the excess reactive oxygen radicals produced in cell metabolism,thus reducing the damage of membrane lipid peroxidation to cells. Glutathione disulfide（GSSG）is the oxidation form of GSH. In recent years,it has been found that the glutathione/glutathione disul?de redox couple（GSH/GSSG）also plays an important role as a signal molecule in plant stress response. This paper reviews the role of GSH/GSSG in plant response to stress,which provides theoretical basis for the regulation of plant resistance by regulating GSH/GSSG.

Key words: glutathione, abiotic stress, signal transduction, defense mechanism

中图分类号:

Q945.78

汪宽鸿, 祝彪, 朱祝军. GSH/GSSG在植物应对非生物胁迫中的作用综述[J]. 园艺学报, 2021, 48(4): 647-660.

WANG Kuanhong, ZHU Biao, ZHU Zhujun. Review of the Role of GSH/GSSG in Plant Abiotic Stress Response[J]. Acta Horticulturae Sinica, 2021, 48(4): 647-660.

图/表 4

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调控对象 Regulation object	研究进展 Research progress	植物种类 Species	参考文献 Reference
SA	调节SA介导的JA信号抑制 Regulate SA to depress JA signal	拟南芥 Arabidopsis thaliana	Koornneef et al.2008
	通过抑制NPR1依赖的SA介导途径表达来减轻生物胁迫 Reduce biotic stress by inhibiting NPR1 dependent SA pathway expression	烟草 Nicotiana tabacumL.	Ghanta et al.2011
	诱导SA信号途径启动和SA积累及PR基因表达 Inducing SA signaling pathway initiation,SA accumulation and PR gene expression	拟南芥 A.thaliana	Mhamdi et al.2010
	在ICS1表达水平上调节SA积累,也独立于NPR1增加细胞内H₂O₂激活SA Regulate SA accumulation at ICS1 expression level,also increase intracellular H₂O₂ to activate SA in NPR1 independent pathway	拟南芥 A.thaliana	Han et al.2013
	通过SA和ET途径减弱AAL毒素 Attenuate AAL toxin through SA and ET pathways	拟南芥 A.thaliana	Sultana et al.2020
ET	发现响应ET的GST基因簇 GST gene cluster responding to ET was found	香石竹 Dianthus caryophyllus	Itzhaki et al.1993
	过表达GSH1,体细胞胚ACO转录增加 Overexpression of GSH1 increased ACO transcription in somatic embryos	白云杉 Picea glauca	Stasolla et al.2004
	外源施加GSSG,ET合成受阻 Exogenous GSSG inhibited ET synthesis	白云杉 Picea glauca	Belmonte et al.2005
	过量GSSG导致 SAM转录降低 Excessive GSSG decreased SAM transcription	芸薹属 Brassica napus L.	Stasolla et al.2008
	通过ACO和SAM的转录调控ET合成 Regulate ET synthesis through regulating transcription of ACO and SAM	芸薹属 B.napusL.	Stasolla,2010
	通过ACS和ACO的转录调控ET合成 Regulate ET synthesis through regulating transcription of ACS and ACO	拟南芥 A.thaliana	Datta et al.2015
	通过SA途径抑制ET合成,缓解AAL诱导的ROS应激 Inhibit ET synthesis through SA pathway and alleviate ROS stress induced by AAL	拟南芥 A.thaliana	Sultana et al.2020
JA	在NPR1水平上调节SA和JA途径的拮抗作用 Regulate antagonism of SA and JA at NPR1 level	拟南芥 A.thaliana	Spoel et al.2003
	参与MeJA信号传导 Participate in MeJA signal transduction	拟南芥 A.thaliana	Akter et al.2010
	独立于NPR1激活氧化应激触发的JA信号 Activate JA signal triggered by oxidative stress independently of NPR1	拟南芥 A.thaliana	Han et al.2013
	增加JA应答基因的表达,增强对干旱和盐胁迫的耐受性 Increase expression of JA response gene,enhance tolerance to drought and salt stress	拟南芥 A.thaliana	Cheng et al.2015
	GSH合成关键转录因子MYC2与在淹水胁迫中激活的JA信号相互作用 Key regulator of GSH biosynthesis MYC interact with JA signal in flooding stress	拟南芥 A.thaliana	Yuan et al.2017
ABA	GSH-ABA相互作用提高对非生物胁迫因子的耐受性 GSH-ABA interaction improves tolerance to abiotic stress factors	拟南芥A.thaliana 小麦Triticum aestivumL.	Wei et al.2015
	外源GSH处理与ABA的积累有关 Exogenous GSH treatment is related to ABA accumulation	拟南芥 A.thaliana	Chen et al.2012
	激活ABA、生长素和JA的生物合成以及信号基因 Activate the biosynthesis of ABA,auxin and JA and signal response genes	拟南芥 A.thaliana	Cheng et al.2015
	影响ABA和MeJA含量,减少叶片气孔孔径 Affect the content of ABA and MeJA,and reduce the stomatal aperture	拟南芥 A.thaliana	Akter et al.2012
	与ABA、ET之间存在串扰 Crosstalk between ABA and ET	拟南芥 A.thaliana	Kumar et al.2016
NO	与NO形成的GSNO连接ROS和活性氮信号通路 GSNO connects ROS and reactive nitrogen signaling pathway	烟草 N. tabacum L.	Clark et al.2001
	镉胁迫激活GSNO信号,增强对镉胁迫的耐受性 Cadmium stress activated GSNO signal and enhanced tolerance to cadmium stress	豌豆 Pisum sativumL.	Barroso et al.2006
	NO通过调节AsA-GSH循环来保护根系免受Al诱导的氧化胁迫 NO alleviates Al-induced oxidative damage through regulating the AsA-GSH cycle	小麦 T. aestivumL.	Sun et al.2015
	过量消耗GSH促进形成S-亚硝基硫醇,参与重金属解毒 Excessive consumption of GSH promotes the formation of S-nitrosothiol	苎麻 Boehmeria nivea	Wang et al.2015
	叶面喷施GSNO缓解水分亏缺对植物的氧化损伤 Foliar spraying of GSNO alleviates oxidative damage of plants under water deficit	甘蔗 Saccharumspp.	Silveira et al.2017
Ca²⁺	通过将Ca²⁺释放到胞浆传递信息参与应激诱导信号通路的早期部分Participate in early signal transduction events by stimulating calcium release into cytosol	烟草 N.tabacum L.	Gomez et al.2004
Ca²⁺	通过谷氧还原蛋白（Grx）影响Ca²⁺信号途径应对冷胁迫 Effect Ca²⁺ signaling pathway on cold stress through glutathione reducing protein	水稻 Oryza sativaL.	Liu et al.2018

调控对象 Regulation object	研究进展 Research progress	植物种类 Species	参考文献 Reference
SA	调节SA介导的JA信号抑制 Regulate SA to depress JA signal	拟南芥 Arabidopsis thaliana	Koornneef et al.2008
	通过抑制NPR1依赖的SA介导途径表达来减轻生物胁迫 Reduce biotic stress by inhibiting NPR1 dependent SA pathway expression	烟草 Nicotiana tabacumL.	Ghanta et al.2011
	诱导SA信号途径启动和SA积累及PR基因表达 Inducing SA signaling pathway initiation,SA accumulation and PR gene expression	拟南芥 A.thaliana	Mhamdi et al.2010
	在ICS1表达水平上调节SA积累,也独立于NPR1增加细胞内H₂O₂激活SA Regulate SA accumulation at ICS1 expression level,also increase intracellular H₂O₂ to activate SA in NPR1 independent pathway	拟南芥 A.thaliana	Han et al.2013
	通过SA和ET途径减弱AAL毒素 Attenuate AAL toxin through SA and ET pathways	拟南芥 A.thaliana	Sultana et al.2020
ET	发现响应ET的GST基因簇 GST gene cluster responding to ET was found	香石竹 Dianthus caryophyllus	Itzhaki et al.1993
	过表达GSH1,体细胞胚ACO转录增加 Overexpression of GSH1 increased ACO transcription in somatic embryos	白云杉 Picea glauca	Stasolla et al.2004
	外源施加GSSG,ET合成受阻 Exogenous GSSG inhibited ET synthesis	白云杉 Picea glauca	Belmonte et al.2005
	过量GSSG导致 SAM转录降低 Excessive GSSG decreased SAM transcription	芸薹属 Brassica napus L.	Stasolla et al.2008
	通过ACO和SAM的转录调控ET合成 Regulate ET synthesis through regulating transcription of ACO and SAM	芸薹属 B.napusL.	Stasolla,2010
	通过ACS和ACO的转录调控ET合成 Regulate ET synthesis through regulating transcription of ACS and ACO	拟南芥 A.thaliana	Datta et al.2015
	通过SA途径抑制ET合成,缓解AAL诱导的ROS应激 Inhibit ET synthesis through SA pathway and alleviate ROS stress induced by AAL	拟南芥 A.thaliana	Sultana et al.2020
JA	在NPR1水平上调节SA和JA途径的拮抗作用 Regulate antagonism of SA and JA at NPR1 level	拟南芥 A.thaliana	Spoel et al.2003
	参与MeJA信号传导 Participate in MeJA signal transduction	拟南芥 A.thaliana	Akter et al.2010
	独立于NPR1激活氧化应激触发的JA信号 Activate JA signal triggered by oxidative stress independently of NPR1	拟南芥 A.thaliana	Han et al.2013
	增加JA应答基因的表达,增强对干旱和盐胁迫的耐受性 Increase expression of JA response gene,enhance tolerance to drought and salt stress	拟南芥 A.thaliana	Cheng et al.2015
	GSH合成关键转录因子MYC2与在淹水胁迫中激活的JA信号相互作用 Key regulator of GSH biosynthesis MYC interact with JA signal in flooding stress	拟南芥 A.thaliana	Yuan et al.2017
ABA	GSH-ABA相互作用提高对非生物胁迫因子的耐受性 GSH-ABA interaction improves tolerance to abiotic stress factors	拟南芥A.thaliana 小麦Triticum aestivumL.	Wei et al.2015
	外源GSH处理与ABA的积累有关 Exogenous GSH treatment is related to ABA accumulation	拟南芥 A.thaliana	Chen et al.2012
	激活ABA、生长素和JA的生物合成以及信号基因 Activate the biosynthesis of ABA,auxin and JA and signal response genes	拟南芥 A.thaliana	Cheng et al.2015
	影响ABA和MeJA含量,减少叶片气孔孔径 Affect the content of ABA and MeJA,and reduce the stomatal aperture	拟南芥 A.thaliana	Akter et al.2012
	与ABA、ET之间存在串扰 Crosstalk between ABA and ET	拟南芥 A.thaliana	Kumar et al.2016
NO	与NO形成的GSNO连接ROS和活性氮信号通路 GSNO connects ROS and reactive nitrogen signaling pathway	烟草 N. tabacum L.	Clark et al.2001
	镉胁迫激活GSNO信号,增强对镉胁迫的耐受性 Cadmium stress activated GSNO signal and enhanced tolerance to cadmium stress	豌豆 Pisum sativumL.	Barroso et al.2006
	NO通过调节AsA-GSH循环来保护根系免受Al诱导的氧化胁迫 NO alleviates Al-induced oxidative damage through regulating the AsA-GSH cycle	小麦 T. aestivumL.	Sun et al.2015
	过量消耗GSH促进形成S-亚硝基硫醇,参与重金属解毒 Excessive consumption of GSH promotes the formation of S-nitrosothiol	苎麻 Boehmeria nivea	Wang et al.2015
	叶面喷施GSNO缓解水分亏缺对植物的氧化损伤 Foliar spraying of GSNO alleviates oxidative damage of plants under water deficit	甘蔗 Saccharumspp.	Silveira et al.2017
Ca²⁺	通过将Ca²⁺释放到胞浆传递信息参与应激诱导信号通路的早期部分Participate in early signal transduction events by stimulating calcium release into cytosol	烟草 N.tabacum L.	Gomez et al.2004
Ca²⁺	通过谷氧还原蛋白（Grx）影响Ca²⁺信号途径应对冷胁迫 Effect Ca²⁺ signaling pathway on cold stress through glutathione reducing protein	水稻 Oryza sativaL.	Liu et al.2018