园艺学报 ›› 2021, Vol. 48 ›› Issue (2): 347-354.doi: 10.16420/j.issn.0513-353x.2020-0241

• 研究报告 • 上一篇    下一篇


范旭东, 董雅凤*(), 张尊平, 任芳, 胡国君, 张梦妍, 李晨   

  1. 中国农业科学院果树研究所,国家落叶果树脱毒中心,辽宁兴城 125100
  • 收稿日期:2020-09-18 修回日期:2020-12-11 出版日期:2021-02-25 发布日期:2021-03-09
  • 通讯作者: 董雅凤
  • 基金资助:

Detection and Sequence Analyses of Grapevine Leafroll-associated Virus 7 Isolates in China

FAN Xudong, DONG Yafeng*(), ZHANG Zunping, REN Fang, HU Guojun, ZHANG Mengyan, LI Chen   

  1. National Center for Eliminating Viruses from Deciduous Fruit Tree,Research Institute of Pomology,Chinese Academy of Agricultural Sciences,Xingcheng,Liaoning 125100,China
  • Received:2020-09-18 Revised:2020-12-11 Online:2021-02-25 Published:2021-03-09
  • Contact: DONG Yafeng


葡萄卷叶相关病毒7(grapevine leafroll-associated virus 7,GLRaV-7)是与葡萄卷叶病相关的重要病原物之一。为明确中国GLRaV-7分离物的基因序列变异,采用RT-PCR方法对采自中国15个省(市)、自治区的188份葡萄样品进行检测,检出率为24.5%(44/188)。对来源于42个GLRaV-7分离物的外壳蛋白(coat protein,CP)和15个分离物的热休克蛋白70(heat-shock protein 70,HSP70)基因进行克隆、测序。序列分析结果显示,来源于国内外GLRaV-7分离物的CP和HSP70基因的核苷酸和氨基酸序列同源性分别为89.12% ~ 100.00%和88.94% ~ 100.00%、85.33% ~ 100.00%和88.97% ~ 100.00%。基于CP和HSP70基因序列构建的进化树,分别将GLRaV-7分离物划分为6个和5个明显区分的组群。Gp3、Gp4和Gp5仅由中国分离物组成。本研究中初次分析国内外GLRaV-7分离物的CP和HSP70基因序列变异,可为进一步开展GLRaV-7分子检测的研发及不同变种的致病性研究提供依据。

关键词: 葡萄, 葡萄卷叶相关病毒7, RT-PCR检测, CP基因, HSP70基因, 进化树分析


Grapevine leafroll disease(GLD)is a virus disease widely distributed in the world and causes great damage on grapevine. Grapevine leafroll-associated virus 7(GLRaV-7) is one of its pathogens. To investigate the genetic variation of GLRaV-7 isolates,42 positive samples were screened out by reverse transcription-PCR(RT-PCR)from 188 grapevine samples which were collected from 15 Chinese provinces and regions. The detection rate of GLRaV-7 was 24.5%. The coat protein(CP)genes of 42 isolates and heat-shock protein 70(HSP70)genes of 15 isolates were sequenced and analyzed respectively,and the results showed identities ranging 89.12%-100.00% and 88.94%-100.00%,85.33%-100.00% and 88.97%-100.00% at the nucleotide and amino acid sequences respectively. Phylogenetic analysis of CP and HSP70 gene sequences showed that all of GLRaV-7 isolates can be divided into six and five groups,respectively. Gp3,Gp4 and Gp5 only included GLRaV-7 Chinese isolates obtained in this study. This is the first report on the genetic variation of CP and HSP70 genes of GLRaV-7 isolates in China,and this study will be helpful for pathogenicity studies and development of reliable diagnostic methods.

Key words: grapevine, grapevine leafroll-associated virus 7, RT-PCR detection, CP gene, HSP70 gene, phylogenetic analysis