园艺学报 ›› 2009, Vol. 36 ›› Issue (10): 1425-1430.

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李广平1*;张长青3*; 章镇2**; 曹福亮1   

  1. (1南京林业大学森林资源与环境学院, 南京210037; 2 南京农业大学园艺学院, 南京210095; 3 金陵科技学院园艺系, 南京210038)
  • 收稿日期:2009-05-25 修回日期:2009-07-13 出版日期:2009-10-25 发布日期:2009-10-25
  • 通讯作者: 章镇

Cloning of pgip Promoter from Prunus salicina and the Computational Identification of Its Regulatory Element

LI Guang-ping1*;ZHANG Chang-qing3*;ZHANG Zhen2**;CAO Fu-liang1   

  1. (1College of Forest Resources and Environm ent, Nanjing Forestry University, Nanjing 210037, China; 2College of Horticulture,Nanjing AgriculturalUniversity, Nanjing 210095, China; 3Department of Horticulture, Jinling Institute of Technology, Nanjing 210038, China)
  • Received:2009-05-25 Revised:2009-07-13 Online:2009-10-25 Published:2009-10-25
  • Contact: ZHANG Zhen

摘要: 在已克隆中国李pgip序列的基础上, 通过染色体步行法获得了该基因上游1 869 bp的启动子
序列, 联合生物信息学中的进化印记法和启动子扫描法对克隆的启动子序列进行了调控元件识别。结果报道了3个调控元件: 1个TGA1结合位点( TGACG) 和2个WRKY结合位点(TGAC) 。这为全面揭示pgip表达的转录调控机制提供了遗传基础。

关键词: 中国李, pgip启动子, 克隆, 调控元件分析

Abstract: Based on the gene sequence of pgip, we have obtained an up stream fragment of 1 869 bp length by genome walker from Prunus sa licina. Using the bioinformatics methods, including the phylogenetic foot printing and the promoter scanning, we identified three cis2regulatory elements from pgip promoter. One is the TGA1 site and the others areWRKY sites. Our discoveries will be help to understand the transcrip tional regulatory mechanism on pgip expression.

Key words: Prunus salicina, promoter of pgip, clone, regulatory element analysis